FastQCFastQC Report
Mon 6 Jun 2016
ERR522950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11192097
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355250.31741147347096793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306210.27359484107401855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199680.17841160597518052No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130610.11669841674888987No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117490.1049758593050078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG196550.056.718022
ACCTGGG223200.048.845963
GTACCTG237800.047.3538781
CCTGGGG198000.037.2860034
TATAACG9100.030.4712352
TGGGGGG225500.028.4459826
CTGGGGG132550.028.291635
ATGGGGG223100.027.1300835
CATGGGG337950.026.906884
GTACATG589200.025.8629551
TACATGG600150.025.0358472
TAACGCA12550.024.7135434
ACATGGG597600.024.3930453
GGGTTAG42350.022.7594781
GTGGTTA71900.022.4952811
GTACACG23350.022.149691
TATCACG10400.021.691392
GTATCAA610500.021.3100971
GGTATCA474300.021.0355631
GTTAAGA100500.019.9662554
Produced by FastQC (version 0.11.5)