FastQCFastQC Report
Mon 6 Jun 2016
ERR522949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4714245
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT517561.097864027007506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181370.3847275650713953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142820.3029541315735606No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT139080.29502072972448395No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC113800.24139602417778458No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC96940.2056320789437121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87960.1865834295841646No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80120.1699529829272768No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG76450.1621680672090653No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA73880.1567165049758763No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC68440.14517701137721947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG44750.042.9699132
ACCTGGG54100.034.589413
GTACCTG57100.033.9263341
GTACATG168800.029.7770121
TACATGG173150.028.7003172
CATGGGG124350.027.2203164
ACATGGG177100.027.1059423
TATCACG4600.026.573562
ATGGGGG85850.025.7923975
CCTGGGG53000.022.3527574
TGGGGGG93750.022.1498476
ATAACGC5000.021.627943
TATAACG5100.020.2808762
GAGTACT156150.019.51220112-13
GGGTTAG18950.019.3535771
AGAGTAC229750.019.12877810-11
AGTACTT162100.017.82503312-13
TAACGCA6400.017.6294084
GAGTACA128750.017.3469141
GGTTAAG34700.017.3435443
Produced by FastQC (version 0.11.5)