##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522949_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4714245
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84062007808249	34.0	31.0	34.0	30.0	34.0
2	32.13858337867463	34.0	31.0	34.0	29.0	35.0
3	31.9730243124827	34.0	31.0	34.0	28.0	35.0
4	34.59263954249302	35.0	35.0	37.0	32.0	37.0
5	34.606955514615805	35.0	34.0	37.0	32.0	37.0
6	34.78403074087155	37.0	35.0	37.0	32.0	37.0
7	34.637856751187094	37.0	35.0	37.0	32.0	37.0
8	34.77603645970882	37.0	35.0	37.0	32.0	37.0
9	36.019960142080016	38.0	35.0	39.0	32.0	39.0
10-11	36.11117421771672	38.0	35.0	39.0	32.0	39.0
12-13	36.15693223411172	38.0	35.0	39.0	32.0	39.0
14-15	37.21480152177072	39.0	36.5	41.0	32.0	41.0
16-17	37.10784759807774	39.0	36.5	41.0	32.0	41.0
18-19	37.146166989624	39.0	36.5	41.0	32.0	41.0
20-21	37.16538586772643	39.0	36.0	41.0	32.0	41.0
22-23	37.064045886456896	39.0	36.0	41.0	32.0	41.0
24-25	37.05491695913131	39.0	36.5	41.0	32.0	41.0
26-27	36.86864428980674	39.0	36.5	41.0	32.0	41.0
28-29	36.67087858183018	38.0	35.0	41.0	31.0	41.0
30-31	36.577355122612424	38.0	36.0	41.0	30.5	41.0
32-33	36.37829620649754	38.0	35.0	41.0	30.0	41.0
34-35	36.248124673197935	38.0	35.0	40.0	30.0	41.0
36-37	36.11933766700712	38.0	35.5	40.0	30.0	41.0
38-39	35.87114172046637	38.0	35.0	40.0	29.0	41.0
40-41	35.83728773112131	38.0	35.0	40.0	29.0	41.0
42-43	35.788272989630364	38.0	35.0	40.0	29.0	41.0
44-45	35.67467314490443	38.0	35.0	40.0	28.0	41.0
46-47	35.38367607114182	38.0	34.5	40.0	27.0	41.0
48-49	35.4759276405872	37.5	35.0	40.0	26.5	41.0
50-51	34.457423405020315	37.5	33.5	39.0	25.0	40.0
52-53	34.64177296682714	37.0	34.5	39.5	25.5	40.0
54-55	35.22506223159806	37.0	34.5	40.0	26.5	41.0
56-57	35.360111852481154	37.0	35.0	40.0	26.5	41.0
58-59	35.34951163972174	38.0	35.0	40.0	26.5	41.0
60-61	35.06145522771939	37.0	35.0	40.0	26.0	41.0
62-63	34.85886902356581	37.0	34.0	40.0	26.0	41.0
64-65	34.6306097795087	36.5	34.0	39.5	26.0	41.0
66-67	34.374990268855356	37.0	34.0	39.0	25.0	41.0
68-69	34.02283875784988	36.0	34.0	39.0	25.0	41.0
70-71	33.60157639240218	36.0	33.0	38.0	23.5	40.0
72-73	33.33648357266116	35.5	33.5	37.5	23.0	40.0
74-75	33.08166365982252	35.0	33.0	37.0	22.0	39.0
76-77	32.744694855698	35.0	33.0	37.0	22.0	39.0
78-79	32.430732280566666	35.0	32.5	37.0	21.5	38.5
80-81	32.19442434578602	35.0	32.0	36.0	20.0	38.0
82-83	31.961691320667466	35.0	32.0	36.0	20.0	38.0
84-85	31.662400448003872	35.0	32.0	35.5	19.5	37.0
86-87	31.574871161766094	35.0	32.0	36.0	19.0	37.0
88-89	31.266264905621156	35.0	31.0	35.0	19.0	37.0
90-91	31.149990507493776	35.0	31.0	35.5	18.0	36.5
92-93	30.90472811659131	35.0	31.0	35.0	18.0	36.0
94-95	30.79803213451995	34.5	30.5	35.0	18.5	36.0
96-97	30.526995414960403	34.0	30.0	35.0	18.0	36.0
98-99	30.477564594118466	34.0	30.0	35.0	18.0	36.5
100	29.241366539074654	34.0	27.0	35.0	14.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03267693863815779
1101	2	0.016043425563907476
1101	3	0.04229157302213338
1101	4	0.06802796715395942
1101	5	0.11614669291504498
1101	6	0.09043187278288656
1101	7	0.10387815598275552
1101	8	0.08655600691708543
1101	9	0.040292743623844274
1101	10-11	0.08198067609331616
1101	12-13	0.017502285693282715
1101	14-15	0.09275196623017479
1101	16-17	-0.03458339865279214
1101	18-19	0.04671558743882542
1101	20-21	0.08853746536784257
1101	22-23	0.09949429904763463
1101	24-25	-0.025399814797481213
1101	26-27	-0.08238358752608832
1101	28-29	-0.03267383092680376
1101	30-31	-0.05462049693019111
1101	32-33	-0.07858277904629318
1101	34-35	-0.16900779381251851
1101	36-37	-0.07199007875359342
1101	38-39	-0.2082435293875946
1101	40-41	-0.1415501714066565
1101	42-43	-0.14631922532201003
1101	44-45	-0.17610105009866572
1101	46-47	-0.20070244144189786
1101	48-49	-0.25580445672100893
1101	50-51	-0.3994616295468134
1101	52-53	-0.3013660691862512
1101	54-55	-0.20468791574314338
1101	56-57	-0.03312173782958183
1101	58-59	0.04431137743862479
1101	60-61	-0.043461160194581794
1101	62-63	-0.10786447516098718
1101	64-65	-0.06796300355060225
1101	66-67	-0.38243985338359465
1101	68-69	-0.1362077757892095
1101	70-71	-0.19343415851481183
1101	72-73	-0.22500060904452113
1101	74-75	-0.2548528242185739
1101	76-77	-0.18519250072284166
1101	78-79	-0.4879312045065376
1101	80-81	-0.20265601356912555
1101	82-83	-0.3313599714194595
1101	84-85	-0.5874262294424071
1101	86-87	-0.9286109302517822
1101	88-89	-0.3510189936609258
1101	90-91	-0.4753666319416361
1101	92-93	-0.4300388527387611
1101	94-95	-0.5351089060765979
1101	96-97	-0.5314989221858148
1101	98-99	-0.6148578368229813
1101	100	-0.6674543750843718
1102	1	-0.006316864850024473
1102	2	0.07631290613120001
1102	3	0.1786826013548648
1102	4	0.1611001722935086
1102	5	0.20366575632831996
1102	6	0.1564823910603863
1102	7	0.2350327236459151
1102	8	0.14936251021636338
1102	9	0.04396281257453438
1102	10-11	0.15161726778163853
1102	12-13	0.1509550428129245
1102	14-15	0.22130867694482248
1102	16-17	0.11899326010900069
1102	18-19	0.07836474943498217
1102	20-21	0.14698635154409345
1102	22-23	-0.09350714967884244
1102	24-25	0.10047718799574312
1102	26-27	0.10442206423712008
1102	28-29	-0.1740170218337198
1102	30-31	0.034638954069237116
1102	32-33	0.07498658818778381
1102	34-35	0.05585105436343696
1102	36-37	-0.014095062741539266
1102	38-39	-0.09477863687829569
1102	40-41	-0.5952348877497826
1102	42-43	0.014345084322577861
1102	44-45	-0.025256641149184134
1102	46-47	-0.07363677665973967
1102	48-49	-0.3286112187980308
1102	50-51	-0.24367642422811997
1102	52-53	-0.3411211224651538
1102	54-55	-0.08688842446302658
1102	56-57	-0.03471171978519294
1102	58-59	0.05230939700251014
1102	60-61	-0.37770457626673704
1102	62-63	-0.15567730993682716
1102	64-65	-0.20699327897448683
1102	66-67	-0.12414356029620421
1102	68-69	-0.24402151986144816
1102	70-71	0.016323435361108807
1102	72-73	-0.14462924438935687
1102	74-75	-0.07071672942642948
1102	76-77	-0.18819825431009463
1102	78-79	-0.10013051655804617
1102	80-81	-0.22318416608615266
1102	82-83	-0.25090189477292313
1102	84-85	-0.16778279658543838
1102	86-87	-0.2564052760008728
1102	88-89	-0.12672309431603068
1102	90-91	-0.16581382991476445
1102	92-93	-0.13771714596082774
1102	94-95	-0.27304360801538863
1102	96-97	-0.17241713140376191
1102	98-99	-0.26386413741778725
1102	100	-0.6247553317471031
1103	1	-0.01432220242067217
1103	2	-0.03480995697226774
1103	3	-0.157997770527313
1103	4	-0.03150007822275569
1103	5	-0.04210715143675969
1103	6	-0.11635221907761206
1103	7	0.054292835988817956
1103	8	-0.15882724957598526
1103	9	-0.2308590804852102
1103	10-11	-0.15269312215480113
1103	12-13	-0.10779240397783241
1103	14-15	-0.1595658381096925
1103	16-17	-0.19189581270584455
1103	18-19	-0.23767555139597363
1103	20-21	-0.12935862882192595
1103	22-23	-0.31665495849561864
1103	24-25	-0.2605979437522521
1103	26-27	-0.42671017588934745
1103	28-29	-0.4332030103034157
1103	30-31	-0.2571534544702345
1103	32-33	-0.4201175592778341
1103	34-35	-0.4027065629959239
1103	36-37	-0.42059451423710215
1103	38-39	-0.2801757566638017
1103	40-41	-0.23588460739687633
1103	42-43	-0.2300754875739912
1103	44-45	-0.3234635279751217
1103	46-47	-0.30909830528422333
1103	48-49	-0.4544255918597102
1103	50-51	-0.4849063544479506
1103	52-53	-0.26027822140162726
1103	54-55	-0.2030785704707796
1103	56-57	-0.20568849026689406
1103	58-59	-0.06649044298288231
1103	60-61	-0.19921433031531421
1103	62-63	-0.11276217795992949
1103	64-65	-0.3084964978160869
1103	66-67	-0.12381944293347402
1103	68-69	-0.09330818281630116
1103	70-71	-0.21722488668958562
1103	72-73	-0.2652432638437645
1103	74-75	-0.22860366365347318
1103	76-77	-0.3227548003145131
1103	78-79	-0.358268912707878
1103	80-81	-0.35645430538411915
1103	82-83	-0.3194129388410971
1103	84-85	-0.3479516780500447
1103	86-87	-0.4820094371178776
1103	88-89	-0.3800302577243819
1103	90-91	-0.341197427796434
1103	92-93	-0.42179632749065377
1103	94-95	-0.5003683044587568
1103	96-97	-0.5096960062308362
1103	98-99	-0.41502372687138944
1103	100	-0.488265768898426
1104	1	0.08864480934953534
1104	2	0.07607463400956505
1104	3	0.10714699642317527
1104	4	0.05999068152293319
1104	5	0.07979027185535159
1104	6	0.01534984543460638
1104	7	0.12150875330635103
1104	8	0.03807499599412978
1104	9	-0.0312436601629571
1104	10-11	-0.02375738568351693
1104	12-13	0.08757212416286819
1104	14-15	0.08981753563777062
1104	16-17	0.05408389861167251
1104	18-19	-0.06966166131120843
1104	20-21	-0.12473756366849642
1104	22-23	-0.05785585243292246
1104	24-25	0.022500729435833478
1104	26-27	0.0223383561899837
1104	28-29	-0.08825897037447561
1104	30-31	-0.016194684586459118
1104	32-33	0.05885605793093873
1104	34-35	0.015476215049893938
1104	36-37	-0.016032182265227846
1104	38-39	-0.08364277011759924
1104	40-41	0.07953020712219683
1104	42-43	0.0020343660836985578
1104	44-45	-0.07030718467125752
1104	46-47	-0.17578716324853616
1104	48-49	-0.13887099140649184
1104	50-51	-0.12075702112488074
1104	52-53	-0.09264722740626041
1104	54-55	-0.14700620407193554
1104	56-57	-0.6212969655467617
1104	58-59	-0.05947339111921934
1104	60-61	0.027098880609919718
1104	62-63	-0.003069615620269417
1104	64-65	-0.11565453817699023
1104	66-67	-0.11064447433239621
1104	68-69	-0.07688511534720988
1104	70-71	-0.08420608774226679
1104	72-73	-0.08660691748058014
1104	74-75	-0.07783411947779939
1104	76-77	-0.17157648089163047
1104	78-79	-0.13529254980704053
1104	80-81	-0.1750249340792891
1104	82-83	-0.06796014641744108
1104	84-85	-0.23183182483970555
1104	86-87	-0.10808899003377803
1104	88-89	-0.16836544380658935
1104	90-91	-0.3891363068056002
1104	92-93	-0.57795537463738
1104	94-95	-0.15059012924723802
1104	96-97	-0.317027624548853
1104	98-99	-0.22532644567413485
1104	100	-0.23446583787744757
1105	1	-0.04127846188087503
1105	2	-0.0013694230691996268
1105	3	0.005084353256549434
1105	4	0.0010971282198752874
1105	5	-0.09886914767812982
1105	6	-0.09735063528261634
1105	7	0.02253442888201107
1105	8	-0.08694308071945756
1105	9	-0.0372928591889945
1105	10-11	-0.10397045367663083
1105	12-13	-0.09316801525082496
1105	14-15	-0.11196261736429136
1105	16-17	-0.019219784483134106
1105	18-19	-0.05576522332524547
1105	20-21	-0.12472835925265002
1105	22-23	-0.18024217789727004
1105	24-25	-0.22378467198107188
1105	26-27	-0.23580484744015706
1105	28-29	-0.24691012491187792
1105	30-31	-0.23643690305943466
1105	32-33	-0.16508106926649901
1105	34-35	-0.21335576355790664
1105	36-37	-0.15432184048723485
1105	38-39	-0.1382704562413224
1105	40-41	-0.21792339597958943
1105	42-43	-0.09017390612408605
1105	44-45	-0.2533695412674106
1105	46-47	-0.04496219138368218
1105	48-49	-0.2153203073381036
1105	50-51	-0.32716841865885726
1105	52-53	-0.13761540100344405
1105	54-55	-0.12930317078991038
1105	56-57	-0.08979347640892144
1105	58-59	-0.043029085426333324
1105	60-61	-0.030794095756554896
1105	62-63	0.003214628127857111
1105	64-65	0.04078001273291676
1105	66-67	-9.24655872509561E-5
1105	68-69	-0.05034195233037053
1105	70-71	-0.13760915954998865
1105	72-73	-0.11626141027931425
1105	74-75	-0.18002377409395365
1105	76-77	-0.1666067605730177
1105	78-79	-0.10534047919304612
1105	80-81	0.04714428104023938
1105	82-83	0.029224915479886704
1105	84-85	-0.03474630348071628
1105	86-87	0.03510880432249053
1105	88-89	-0.00819057888376662
1105	90-91	-0.04484921253530061
1105	92-93	-0.08526999105995614
1105	94-95	-0.1134518295780289
1105	96-97	-0.044716927119825556
1105	98-99	-0.1674630752637718
1105	100	-0.055135444896336594
1106	1	0.08357059603660844
1106	2	0.05129694022829767
1106	3	0.0729701702213319
1106	4	0.12178057045441193
1106	5	0.15854899087094765
1106	6	0.13705546078424646
1106	7	0.2329817704183128
1106	8	0.051560391338831835
1106	9	0.07919130220315651
1106	10-11	0.05182515278583111
1106	12-13	0.044357904166268725
1106	14-15	0.13162354973651702
1106	16-17	0.025182815551069382
1106	18-19	0.03778201068463005
1106	20-21	0.031683140508611984
1106	22-23	0.069108240680535
1106	24-25	0.13971959463008687
1106	26-27	0.06596801129494878
1106	28-29	0.04597122785986585
1106	30-31	0.09682957454345598
1106	32-33	0.04460361632846599
1106	34-35	0.0699373568266779
1106	36-37	0.12982030327704308
1106	38-39	0.07152826835893222
1106	40-41	0.11704365441783438
1106	42-43	0.06545440791921919
1106	44-45	0.05960212690600031
1106	46-47	0.10098823898841403
1106	48-49	-0.07344760958950758
1106	50-51	-0.006104022766137973
1106	52-53	0.04317006457606709
1106	54-55	0.10913456924085096
1106	56-57	0.04642329084293095
1106	58-59	0.12169288628464159
1106	60-61	0.14627074967856402
1106	62-63	0.20006744552049582
1106	64-65	0.1440609098630219
1106	66-67	0.22402405591353158
1106	68-69	0.15520341131122706
1106	70-71	0.19923850105670482
1106	72-73	0.1492100268766947
1106	74-75	0.11649465595039032
1106	76-77	0.1379194535175543
1106	78-79	0.18583837181184748
1106	80-81	0.05058963224455226
1106	82-83	0.16416552977121768
1106	84-85	0.11001071306417387
1106	86-87	-0.03292754137359566
1106	88-89	0.023465877395711487
1106	90-91	0.04539463344599781
1106	92-93	0.08008727849261277
1106	94-95	0.17049578651533537
1106	96-97	0.071189346320633
1106	98-99	0.0077746200515633745
1106	100	0.08721660246893492
1107	1	-0.11028111980418487
1107	2	-0.12659028650683268
1107	3	-0.03787565092591905
1107	4	0.07929828269404737
1107	5	0.045614797886258884
1107	6	0.02497055238992374
1107	7	0.13055473814216612
1107	8	-0.054229851692731756
1107	9	-0.039600920622781643
1107	10-11	-0.07285113840696766
1107	12-13	-0.04986195356123346
1107	14-15	-0.03632042593593354
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2316	68-69	-0.07693270345583869
2316	70-71	-0.14613288462795992
2316	72-73	-0.09330021755294382
2316	74-75	-0.020993383187629888
2316	76-77	-1.411026696993794
2316	78-79	-0.024439418878905883
2316	80-81	-1.0391775072052667
2316	82-83	-0.0630628085266558
2316	84-85	-1.6704180668784758
2316	86-87	-0.01160898899075491
2316	88-89	0.14170128819413108
2316	90-91	-0.717621592518956
2316	92-93	0.11249821174590124
2316	94-95	0.04456783502473982
2316	96-97	0.18410764519314782
2316	98-99	0.13718183878541268
2316	100	0.08265892771309069
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	10894.0
1	929.0
2	603.0
3	495.0
4	460.0
5	487.0
6	520.0
7	904.0
8	1541.0
9	1907.0
10	2855.0
11	5480.0
12	9119.0
13	11568.0
14	13062.0
15	14662.0
16	16242.0
17	19524.0
18	23812.0
19	28486.0
20	33208.0
21	39581.0
22	45294.0
23	49280.0
24	50161.0
25	51203.0
26	53931.0
27	58244.0
28	66249.0
29	77770.0
30	94094.0
31	116626.0
32	152820.0
33	210392.0
34	330284.0
35	585952.0
36	712847.0
37	763243.0
38	842584.0
39	216651.0
40	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.67573020003754	17.878414740400125	12.61535865043478	22.83049640912756
2	15.93402529994972	21.413789717916142	39.22607893281696	23.426106049317177
3	20.616359097465814	26.17027641307067	29.87417962325212	23.33918486621139
4	12.731429388980212	16.277282858603378	38.52009527031472	32.471192482101685
5	12.786551544251509	38.170469184171026	34.0148197042447	15.028159567332771
6	29.49978743423547	36.48520061399376	18.831347896747936	15.183664055022831
7	26.822650202005928	32.92511479350789	22.70815208415977	17.54408292032641
8	25.80313130077192	32.96052092587765	20.82933187213057	20.407015901219854
9	26.814742297112517	16.84946448238618	21.25746878142475	35.07832443907656
10-11	25.185692832671556	25.13756482493703	28.898857991738662	20.77788435065275
12-13	26.533874146813325	23.468381370533198	27.98738484892396	22.010359633729518
14-15	23.251063040322983	25.16061025514244	27.580391294153987	24.007935410380586
16-17	22.039679957178418	27.63523832429498	27.704157499515738	22.620924219010863
18-19	21.66978936919281	28.02910124684671	28.73539591293033	21.56571347103015
20-21	23.183827354339055	26.162583403052004	28.254366899693533	22.399222342915408
22-23	22.264281903988596	26.116817538929855	28.683944046296006	22.934956510785543
24-25	23.024908082495035	25.951447384842318	28.49669807702313	22.526946455639514
26-27	22.465073641076575	25.388599327499833	29.7828294901333	22.363497541290293
28-29	21.752398667504487	28.010860444984548	28.49331278156044	21.743428105950528
30-31	23.17207446534039	25.87762821398747	28.574681158675542	22.375616161996597
32-33	23.061271042124996	26.132111063139728	28.754973070888962	22.05164482384631
34-35	23.59633310983243	26.191397775786534	28.516782463683587	21.69548665069745
36-37	22.99109954952961	25.95467460920076	29.230363423711154	21.82386241755847
38-39	22.539079513206126	27.072676545886164	28.744746913856783	21.643497027050927
40-41	22.63894227049019	26.87298107117688	28.354863784404344	22.13321287392858
42-43	22.097380110323463	27.23698051389088	27.33403119879994	23.331608176985718
44-45	22.345427855338954	26.382084421427525	28.12370537803121	23.14878234520231
46-47	23.028284369822572	26.625305405740118	27.909839501596558	22.436570722840756
48-49	22.718314683972913	26.72689757202344	28.02434478750669	22.53044295649696
50-51	21.628304078607826	27.083625735163373	29.084808785430454	22.20326140079834
52-53	22.49072922964262	27.778634014254212	27.20727356329669	22.523363192806478
54-55	21.784845403608717	26.505062172454785	28.185665748135964	23.524426675800527
56-57	22.759017986102766	26.41534739733149	27.6496825653138	23.175952051251947
58-59	23.685123025957708	26.514753046620083	27.50453339792942	22.295590529492788
60-61	23.019792181877463	26.760094868308926	28.795732628688132	21.42438032112548
62-63	22.363110269382666	26.91095571254354	28.40056529297805	22.325368725095743
64-65	21.950340034257795	27.710243003855545	27.646406092582893	22.69301086930377
66-67	22.490092111807762	26.53392157417779	27.204344168142836	23.771642145871613
68-69	21.688883212805244	26.85857505467665	27.87206462221229	23.580477110305818
70-71	22.796150625726632	26.543208168136662	27.730314301413678	22.930326904723024
72-73	22.303880944967386	26.393236090153525	28.418757767611684	22.884125197267398
74-75	21.73882453745466	28.044683223301693	27.4272287726749	22.789263466568745
76-77	22.02483790147892	26.40146072249018	27.447004963365934	24.12669641266496
78-79	22.07501627914947	26.590583062830177	28.033919382714235	23.30048127530612
80-81	22.774429470469272	26.50585211307411	27.468265593845796	23.25145282261082
82-83	22.516390267363594	26.27680254618001	28.313063128517136	22.893744057939262
84-85	22.101323701664068	27.240189113619323	27.71499560809847	22.94349157661814
86-87	21.797598548182158	27.478775764758506	27.52070425385225	23.202921433207084
88-89	21.354664622046247	27.037501039037696	27.24233913913718	24.365495199778877
90-91	22.54193602502217	25.992437315770804	27.426641494620068	24.038985164586947
92-93	22.84194750782649	26.669774983674415	27.56877816535367	22.91949934314543
94-95	22.327407485626427	26.35506290819861	27.57050959707159	23.74702000910337
96-97	21.06525515814266	26.397364801788992	28.711158169911815	23.82622187015653
98-99	21.78613555363973	26.840222310242883	27.25490110502595	24.118741031091435
100	21.53552557441881	25.48338656146817	27.5777032105956	25.403384653517424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7746.0
1	6036.0
2	4147.5
3	3383.0
4	2210.0
5	1813.0
6	2423.0
7	3826.0
8	5838.5
9	7016.0
10	6284.5
11	5292.0
12	5352.5
13	5732.0
14	6171.0
15	6545.0
16	6828.0
17	7243.5
18	7809.5
19	8297.5
20	8837.0
21	9701.5
22	10942.5
23	12883.0
24	15457.0
25	18823.5
26	23133.0
27	28159.5
28	33810.0
29	40469.0
30	48331.0
31	55607.0
32	63162.0
33	71439.0
34	79470.5
35	87200.5
36	96853.5
37	106566.0
38	113393.5
39	120194.0
40	125459.5
41	129914.0
42	134324.0
43	139232.5
44	145536.5
45	153181.0
46	164048.5
47	199871.0
48	272123.5
49	275246.5
50	215355.0
51	188119.0
52	175357.5
53	165131.5
54	157042.0
55	145979.5
56	132578.5
57	119927.0
58	105728.5
59	89411.0
60	73071.5
61	59149.5
62	47837.0
63	37375.0
64	27742.0
65	20785.5
66	15612.0
67	11872.5
68	9070.5
69	6790.0
70	5246.0
71	3988.5
72	3040.0
73	2319.5
74	1723.5
75	1275.5
76	949.0
77	614.0
78	406.0
79	294.5
80	209.5
81	150.0
82	122.5
83	100.0
84	75.5
85	60.5
86	51.5
87	37.0
88	26.0
89	22.0
90	24.0
91	19.0
92	14.0
93	12.5
94	7.5
95	7.5
96	6.5
97	5.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20529268207316337
2	0.18291370092135645
3	0.37057895803039514
4	0.36684559245435905
5	0.3774729569634162
6	0.3080662969362008
7	0.3260967556841021
8	0.3164875817866912
9	0.32921496443226855
10-11	0.3028904946603327
12-13	0.31092995803145573
14-15	0.2979692400373761
16-17	0.29297374234898693
18-19	0.31372998221348275
20-21	0.3182163845960488
22-23	0.30914812446107487
24-25	0.31365573914805017
26-27	0.30377080529331846
28-29	0.3296922412814777
30-31	0.31159814562034854
32-33	0.31148147794609743
34-35	0.31364513299584557
36-37	0.3215148979316943
38-39	0.3279316200155062
40-41	0.35368335756839114
42-43	0.3214512610184664
44-45	0.30787538619651716
46-47	0.31307240077679455
48-49	0.3357165357337177
50-51	0.32612857414071605
52-53	0.35392729906909803
54-55	0.3543833636138979
56-57	0.3601743227176356
58-59	0.3464817802214353
60-61	0.3292361767366779
62-63	0.3500878719710155
64-65	0.34652420483025387
66-67	0.3333725760964905
68-69	0.3430029622983108
70-71	0.36200918704903967
72-73	0.3660713433434198
74-75	0.3549348835285395
76-77	0.33986354124573503
78-79	0.35038484423274563
80-81	0.3626349500291139
82-83	0.3675349923476612
84-85	0.3598243196948822
86-87	0.3597925012382683
88-89	0.3476378508117418
90-91	0.3599940181301566
92-93	0.34145446407643215
94-95	0.3623697962239977
96-97	0.37165017940306455
98-99	0.36305919611729975
100	0.38409119593911645
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4714245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.24465095285221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15009702228673	47.081253170391996
2	13.320452347454928	16.04972004413046
3	3.9505059278689685	7.139905521349188
4	1.5732267086119154	3.791139757201175
5	0.8227376710279349	2.4782771908420242
6	0.524013569623448	1.8941408757913651
7	0.3227682394073662	1.361154194523439
8	0.22860686938181532	1.1017872841065393
9	0.19258364740235256	1.0441921155283704
>10	0.8517732729224231	8.882180362396996
>50	0.041272600622142344	1.6835186043684316
>100	0.01962861397003705	2.2190843234835365
>500	0.0012472206274498226	0.5479283749262817
>1k	6.437266918178594E-4	0.8999151901456324
>5k	2.413975094316954E-4	1.1726189663880462
>10k+	2.011645911930795E-4	2.6531840244266047
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	51756	1.097864027007506	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18137	0.3847275650713953	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14282	0.3029541315735606	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13908	0.29502072972448395	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11380	0.24139602417778458	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	9694	0.2056320789437121	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8796	0.1865834295841646	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	8012	0.1699529829272768	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	7645	0.1621680672090653	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	7388	0.1567165049758763	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	6844	0.14517701137721947	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1212304409295656E-5
2	0.0	0.0	0.0	0.0	2.1212304409295656E-5
3	0.0	0.0	0.0	0.0	2.1212304409295656E-5
4	0.0	0.0	0.0	0.0	4.242460881859131E-5
5	0.0	0.0	0.0	0.0	4.242460881859131E-5
6	0.0	0.0	0.0	0.0	4.242460881859131E-5
7	0.0	0.0	0.0	0.0	4.242460881859131E-5
8	0.0	0.0	0.0	0.0	4.242460881859131E-5
9	0.0	0.0	0.0	2.1212304409295656E-5	6.363691322788697E-5
10-11	0.0	0.0	0.0	5.303076102323914E-5	1.0606152204647828E-4
12-13	0.0	0.0	0.0	8.484921763718262E-5	1.0606152204647828E-4
14-15	0.0	0.0	0.0	1.1666767425112611E-4	1.0606152204647828E-4
16-17	0.0	0.0	0.0	1.3787997866042178E-4	1.2727382645577395E-4
18-19	0.0	0.0	0.0	1.9091073968366092E-4	1.484861308650696E-4
20-21	0.0	0.0	0.0	2.7575995732084357E-4	1.484861308650696E-4
22-23	0.0	0.0	0.0	4.2424608818591314E-4	1.590922830697174E-4
24-25	0.0	0.0	0.0	8.909167851904175E-4	1.909107396836609E-4
26-27	0.0	0.0	0.0	0.0016651658961297092	2.0151689188830873E-4
28-29	0.0	0.0	0.0	0.004231854729654483	2.1212304409295657E-4
30-31	0.0	0.0	0.0	0.011921315078024158	2.1212304409295657E-4
32-33	0.0	0.0	0.0	0.03164875817866912	2.545476529115479E-4
34-35	0.0	0.0	0.0	0.059808092282009104	2.757599573208435E-4
36-37	0.0	1.0606152204647828E-5	0.0	0.10146905814186577	3.1818456613943487E-4
38-39	2.1212304409295656E-5	2.1212304409295656E-5	0.0	0.16814993705248665	3.1818456613943487E-4
40-41	2.1212304409295656E-5	3.181845661394349E-5	0.0	0.24625364188751325	3.1818456613943487E-4
42-43	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.32347703608955414	3.287907183440827E-4
44-45	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.4186248275174498	3.393968705487305E-4
46-47	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.5288333550759454	3.393968705487305E-4
48-49	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.6419585744907191	3.393968705487305E-4
50-51	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.7684687579877584	3.393968705487305E-4
52-53	2.1212304409295656E-5	8.484921763718262E-5	0.0	0.9044926600123668	3.924276315719696E-4
54-55	3.181845661394349E-5	8.484921763718262E-5	0.0	1.067594068615441	4.136399359812653E-4
56-57	4.242460881859131E-5	8.484921763718262E-5	0.0	1.2519289939322205	4.2424608818591314E-4
58-59	4.242460881859131E-5	8.484921763718262E-5	0.0	1.4548458979115426	4.2424608818591314E-4
60-61	4.242460881859131E-5	8.484921763718262E-5	0.0	1.6653992314782111	4.2424608818591314E-4
62-63	4.242460881859131E-5	1.0606152204647828E-4	0.0	1.9010891457698953	4.2424608818591314E-4
64-65	4.242460881859131E-5	1.0606152204647828E-4	0.0	2.133066906789953	4.2424608818591314E-4
66-67	4.242460881859131E-5	1.0606152204647828E-4	0.0	2.4073738212587594	4.2424608818591314E-4
68-69	4.242460881859131E-5	1.0606152204647828E-4	0.0	2.7367796964307116	4.2424608818591314E-4
70-71	4.242460881859131E-5	1.0606152204647828E-4	0.0	3.0812357015810594	4.4545839259520876E-4
72-73	4.242460881859131E-5	1.0606152204647828E-4	0.0	3.4074491249394123	4.6667069700450444E-4
74-75	4.242460881859131E-5	1.0606152204647828E-4	0.0	3.7479384291652216	4.6667069700450444E-4
76-77	4.242460881859131E-5	1.0606152204647828E-4	0.0	4.124668955474313	4.6667069700450444E-4
78-79	4.242460881859131E-5	1.0606152204647828E-4	0.0	4.581550173993927	4.6667069700450444E-4
80-81	4.242460881859131E-5	1.0606152204647828E-4	0.0	5.0327889195406685	4.772768492091523E-4
82-83	4.242460881859131E-5	1.0606152204647828E-4	0.0	5.497624752213769	4.878830014138001E-4
84-85	4.242460881859131E-5	1.0606152204647828E-4	0.0	5.984701686060016	4.878830014138001E-4
86-87	4.242460881859131E-5	1.0606152204647828E-4	0.0	6.48254386439398	5.090953058230958E-4
88	4.242460881859131E-5	1.0606152204647828E-4	0.0	6.884092786861947	5.090953058230958E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4475	0.0	42.969913	2
ACCTGGG	5410	0.0	34.58941	3
GTACCTG	5710	0.0	33.926334	1
GTACATG	16880	0.0	29.777012	1
TACATGG	17315	0.0	28.700317	2
CATGGGG	12435	0.0	27.220316	4
ACATGGG	17710	0.0	27.105942	3
TATCACG	460	0.0	26.57356	2
ATGGGGG	8585	0.0	25.792397	5
CCTGGGG	5300	0.0	22.352757	4
TGGGGGG	9375	0.0	22.149847	6
ATAACGC	500	0.0	21.62794	3
TATAACG	510	0.0	20.280876	2
GAGTACT	15615	0.0	19.512201	12-13
GGGTTAG	1895	0.0	19.353577	1
AGAGTAC	22975	0.0	19.128778	10-11
AGTACTT	16210	0.0	17.825033	12-13
TAACGCA	640	0.0	17.629408	4
GAGTACA	12875	0.0	17.346914	1
GGTTAAG	3470	0.0	17.343544	3
>>END_MODULE