FastQCFastQC Report
Mon 6 Jun 2016
ERR522949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4714245
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT533051.130721886537505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170030.36067281187125405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145220.30804508463179153No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT141060.2992207659975245No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC119660.25382643456163184No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC102480.2173836955864619No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA85450.1812591411774314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85220.18077125817601758No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG82680.17538333285605648No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA79420.16846812161862612No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC71660.15200737339701267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG46450.041.7877432
TATAACG4100.035.5354962
GTACCTG56750.035.217711
ACCTGGG55700.034.592063
ATAACGC4850.028.0998483
GTACATG176250.026.6013741
ATGGGGG84150.025.9684815
TACATGG180650.025.5740872
CATGGGG125250.025.1388154
CCTGGGG52350.024.596974
ACATGGG183150.024.5044063
TAACGCA5400.024.3675584
TATCACG4350.023.7693882
TGGGGGG87350.023.0265356
GGGTTAG18300.020.8147851
GAGTACT154850.020.38633312-13
AGAGTAC240900.018.90979210-11
AGTACTT162250.018.0512712-13
GTACTTT173950.018.05051414-15
CTGGGGG34600.017.9285665
Produced by FastQC (version 0.11.5)