FastQCFastQC Report
Mon 6 Jun 2016
ERR522948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430672
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT356260.4794451968812511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310290.41757999814821595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250480.3370892969034295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154760.20827187635250216No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141040.18980786663709553No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT85360.11487520913317127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123150.053.1496472
GTACCTG141600.046.768061
ACCTGGG145400.043.949163
CCTGGGG135450.032.5595474
TATAACG7850.031.7469312
ATAACGC9700.026.176863
TAACGCA10550.025.4025254
TATCACG8000.024.6862352
CTGGGGG82600.022.2547075
GTACATG346500.021.2025321
CATGGGG192950.020.8341524
GTATAAC18400.020.705091
TACATGG355950.020.4228342
GAGTACT265250.020.40912412-13
ACATGGG355950.019.7491193
ATGGGGG116650.018.4589045
AGTACTT278650.017.99420512-13
TGGGGGG117050.017.463766
TATACCG6200.017.4405965
GTACTTT314350.017.17687414-15
Produced by FastQC (version 0.11.5)