FastQCFastQC Report
Mon 6 Jun 2016
ERR522948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430672
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT374740.5043150875183294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276650.37230818423959505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243220.32731898272457727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150290.20225626968866342No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125650.16909641550589233No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT83870.11287000691189168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG128450.053.0545542
GTACCTG144300.047.74321
ACCTGGG151750.044.2526933
CCTGGGG132550.033.5388074
TATAACG7950.027.1943992
CTGGGGG80500.025.1020245
ATAACGC9400.023.996593
TATCACG7750.021.2253632
TAACGCA10300.020.987314
GTACATG351050.019.892881
GAGTACT261100.019.56945212-13
TGGGGGG105650.019.5266746
CATGGGG192050.019.5265164
TACATGG362150.019.0383992
ACATGGG359650.018.2799023
ATGGGGG109700.018.077655
GTATAAC18350.017.9391611
GTATAGG22350.017.674291
AGTACTT280150.017.66825912-13
TACCGGG11450.017.6502842
Produced by FastQC (version 0.11.5)