FastQCFastQC Report
Mon 6 Jun 2016
ERR522947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330597
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265640.25713905982393853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211930.20514787286736672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118590.11479491456302089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159900.047.200091
GTATCAA273850.039.4155541
TCAACGC328000.032.2811664
ATCAACG334050.031.6853753
CAACGCA335800.031.5155075
TATCAAC347100.030.620422
AACGCAG349950.030.3150026
GTACATG360600.027.3366491
ACGCAGA401700.026.3278547
TACATGG368850.026.3169422
CGCAGAG418200.025.1651468
ACATGGG383750.024.6300683
CATGGGG283250.023.0459294
GCAGAGT468250.022.162879
ATGGGGG163100.020.9755825
GAGTACT247450.020.70422212-13
TATACCG7300.020.5998345
CAGAGTA447300.019.60791410-11
AGAGTAC410000.019.43246810-11
GTACTTT267200.018.87657514-15
Produced by FastQC (version 0.11.5)