FastQCFastQC Report
Mon 6 Jun 2016
ERR522947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330597
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237720.23011254819058374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203610.1970941272803498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114750.11107780121516694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159050.046.32291
GTATCAA268750.038.4783861
TCAACGC323900.031.2807354
ATCAACG329400.030.7736023
CAACGCA329950.030.764145
TATCAAC340450.030.075172
AACGCAG344050.029.5437566
GTACATG344100.025.9096891
ACGCAGA395800.025.6451077
TACATGG354200.024.7647112
CGCAGAG416600.024.3146538
ACATGGG362700.023.52993
GCAGAGT468750.021.4592579
CATGGGG270250.021.1275794
GAGTACT250050.020.58438312-13
ATGGGGG154900.019.4163555
AGAGTAC407100.019.31148310-11
CAGAGTA451950.018.9291710-11
GTACTTT272250.018.67044314-15
GTGGTAT89700.017.7280881
Produced by FastQC (version 0.11.5)