FastQCFastQC Report
Mon 6 Jun 2016
ERR522946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15217043
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT522670.3434767188342702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430340.28280133006130037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250160.16439461990085721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG169450.035.2409252
GTACCTG202450.030.131381
ACCTGGG208000.028.2800733
GTATCAA729300.025.9184271
GGTATCA528450.025.1159691
CATGGGG434450.022.9755884
GTACATG729750.022.957081
TCAACGC835000.022.2585454
TACATGG748100.022.0990872
CAACGCA841150.022.0721385
ATCAACG855900.021.7275123
TATCAAC857100.021.7189642
AACGCAG860700.021.6696036
ACATGGG757400.021.1387043
GAGTACT439550.020.97467612-13
ATGGGGG255250.020.389785
ACGCAGA950450.019.5341157
GTACTTT475150.019.23568714-15
CGCAGAG978800.018.9533638
CCTGGGG219300.018.7382584
Produced by FastQC (version 0.11.5)