FastQCFastQC Report
Mon 6 Jun 2016
ERR522946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15217043
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478290.3143120512966941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430190.2827027563765181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250170.1644011914798427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG172000.035.7132532
GTACCTG207200.030.3175831
ACCTGGG214200.028.1260033
GGTATCA557950.024.5146831
GTATCAA747500.023.7833631
CATGGGG419900.021.7005834
GTACATG735350.021.394871
TACATGG758850.020.5495682
TCAACGC857450.020.3715114
GAGTACT438900.020.28534112-13
CAACGCA864700.020.1952065
TATCAAC879900.019.9391652
AACGCAG884150.019.8359176
ATCAACG882250.019.8201753
ACATGGG767450.019.7233243
CCTGGGG215700.019.3029234
ATGGGGG242600.019.0608545
GTACTTT477600.018.45728714-15
ACGCAGA973300.017.975647
AGTACTT462900.017.83221412-13
Produced by FastQC (version 0.11.5)