FastQCFastQC Report
Mon 6 Jun 2016
ERR522945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390563
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170260.3158482703940201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141840.2631265045970152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.14525384454276855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65310.12115617608030924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG52950.029.9273192
GGTATCA177900.026.3525751
GTACATG197950.025.726271
TACATGG196950.025.2122632
GTATCAA242250.024.9426861
ACCTGGG62050.024.7804853
ACATGGG194750.024.410553
TATAACG4200.023.511152
CATGGGG119500.023.334084
TAACGCA5300.023.0675434
TCAACGC274050.021.7738724
GTACCTG75250.021.6208841
ATCAACG278500.021.4597283
CAACGCA277000.021.4567075
TATCAAC285250.021.0178572
AACGCAG285050.020.9105266
ATGGGGG67650.020.7825665
GAGTACT161250.020.31548512-13
CCGTATC14800.019.05000194
ACGCAGA315750.018.8157737
Produced by FastQC (version 0.11.5)