FastQCFastQC Report
Mon 6 Jun 2016
ERR522945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390563
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160620.2979651661616792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140880.26134561454898125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.14013378565467097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67700.12558985026239372No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT65370.12126748170831136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54850.034.1921772
ACCTGGG65350.028.0497743
GTACCTG76900.024.4655171
GTATCAA239350.024.288781
GGTATCA178400.024.0184081
GTACATG188350.023.7484681
TACATGG191100.022.751582
ACATGGG184550.022.3864483
TATAACG4750.021.7700582
CATGGGG113850.021.5087454
TCAACGC273150.021.0443554
CAACGCA276000.020.793575
ATCAACG278150.020.6660633
ATAACGC5500.020.5096823
TATCAAC283800.020.3549482
AACGCAG284850.020.2941596
GAGTACT153950.020.07197812-13
ATGGGGG64600.019.9361155
CCTGGGG65450.019.1739674
TATACCG4001.8189894E-1218.8010645
Produced by FastQC (version 0.11.5)