FastQCFastQC Report
Mon 6 Jun 2016
ERR522944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783954
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158440.5691185989423675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123190.4425001275164747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.2550329495386777No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60520.21738864938141939No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35880.12888143985137684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA10750.022.7463761
GAGTACT123700.021.67724812-13
GTATCAA299750.021.241091
AACGCAG297300.021.029726
CCGTATC35600.020.85546794
CGTATCA25700.020.47848194
CAACGCA312200.020.1067685
GGTATCA238100.019.927331
TCAACGC317750.019.8595144
TATCAAC317350.019.855272
AGTACTT123950.019.6237512-13
ATCAACG320050.019.5992623
GTACTTT135650.019.59488514-15
ATCATTA18100.019.47132594
TATATGG7750.019.415912
ACGCAGA322200.019.2996167
GTATCAT22650.019.0867794
CGCAGAG327350.019.0534178
GCAGAGT336950.018.2176369
CATGGGG63700.018.1592274
Produced by FastQC (version 0.11.5)