FastQCFastQC Report
Mon 6 Jun 2016
ERR522944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783954
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT1586825.699878661788234No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT943603.3894238195027646RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT531641.9096579900386286RNA PCR Primer, Index 46 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT199810.7177201922158196RNA PCR Primer, Index 46 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG138960.4991461784210515TruSeq Adapter, Index 4 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.4912078288649885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115930.41642211042280153No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC97650.3507601059500265No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT78490.2819371297083213No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC71030.25514070993989124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67070.24091633697970585No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC55230.198386898634101TruSeq Adapter, Index 18 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC49620.17823570360717167No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG45010.16167652195402654TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG43920.15776122737660175RNA PCR Primer, Index 46 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34840.1251457459426413No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG33100.1188956426722568RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA14000.043.0019041
CTTTACA21900.031.570321
AGACTAG13300.026.3249214-15
GTATAAG9250.024.3893898
TTACACA28000.024.1724953
GACTAGG12450.023.78119316-17
TTTACAC29950.023.6972052
ACTAGGC15000.023.49808316-17
CTTATAA10200.023.0555551
GTATTAT3800.022.2789481
TGTATAA10250.022.0103327
GAGTACT117100.021.61317412-13
TATAAGA10600.021.2831929
GTATCAA301250.021.1084041
GGTATCA236900.021.064741
AACGCAG298250.020.7850066
AGTACTT118950.020.62508612-13
TAGGCAT17400.020.52706118-19
GTACCCA8950.020.4949721
TAGATCG1150.00395860620.436055
Produced by FastQC (version 0.11.5)