FastQCFastQC Report
Mon 6 Jun 2016
ERR522943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994348
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185580.30959163532047185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144790.24154420130429533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85070.14191701916538713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG67400.039.263152
GTACCTG83200.032.68471
GGTATCA138800.032.3030431
GTATCAA209450.032.031641
ACCTGGG82850.030.6305183
GTACATG193700.028.8068031
TACATGG200000.027.332942
TCAACGC245950.026.843944
CAACGCA248450.026.4603425
ATCAACG249150.026.4447943
TATCAAC254950.025.8850332
ACATGGG206300.025.7641873
AACGCAG257100.025.674616
CATGGGG147200.023.9106164
ACGCAGA288600.022.8401137
ATGGGGG89750.022.1473945
CCTGGGG81500.022.140654
CGCAGAG298450.022.054088
TAACGCA7000.021.4817034
TATCACG6800.021.4283812
Produced by FastQC (version 0.11.5)