FastQCFastQC Report
Mon 6 Jun 2016
ERR522943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994348
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168260.2806977506144121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140590.23453760108689053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81810.1364785628061634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG66600.042.230452
GTACCTG82850.034.9812741
ACCTGGG80300.034.2372133
GGTATCA145900.033.4081081
GTATCAA216500.031.0108381
TCAACGC253950.025.9626734
ATCAACG257350.025.5294283
GTACATG196950.025.5130671
CAACGCA259800.025.4865955
TATCAAC264050.025.0436652
AACGCAG269700.024.7423026
TACATGG202350.024.3123662
ACATGGG207400.023.0446593
CCTGGGG83200.022.6496264
TATCACG6100.022.3597492
ACGCAGA298200.022.3301337
CGCAGAG306550.021.7579968
CATGGGG147700.021.4128534
ATGGGGG86250.020.1051465
GAGTACT174450.019.9391112-13
Produced by FastQC (version 0.11.5)