##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522943_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5994348
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66805931187179	34.0	31.0	34.0	30.0	35.0
2	32.71933060943409	34.0	31.0	34.0	31.0	34.0
3	32.99679581499105	34.0	31.0	34.0	31.0	36.0
4	35.78328101738504	37.0	35.0	37.0	33.0	37.0
5	35.83951232060601	37.0	35.0	37.0	35.0	37.0
6	35.913450470343065	37.0	35.0	37.0	35.0	37.0
7	35.7397478424676	37.0	35.0	37.0	35.0	37.0
8	35.78860720131697	37.0	36.0	37.0	34.0	37.0
9	37.006733009161294	39.0	36.0	39.0	34.0	39.0
10-11	37.12517499818162	38.5	36.0	39.0	34.0	39.0
12-13	36.99082969490593	38.0	36.5	39.0	33.5	39.0
14-15	38.08314215324168	39.0	36.5	41.0	34.5	41.0
16-17	38.10248879444437	39.0	37.0	41.0	34.5	41.0
18-19	38.10463548329193	39.0	37.0	41.0	34.0	41.0
20-21	37.98930951289448	39.0	36.5	41.0	34.0	41.0
22-23	37.90586265595525	39.0	36.5	41.0	34.0	41.0
24-25	37.77127704297448	39.0	36.0	41.0	34.0	41.0
26-27	37.64572210355488	39.0	36.5	41.0	34.0	41.0
28-29	37.6023439079613	39.0	36.5	41.0	34.0	41.0
30-31	37.44482510858562	38.0	36.0	41.0	34.0	41.0
32-33	37.29614430126512	38.0	36.5	41.0	33.0	41.0
34-35	37.11529485775601	38.0	36.0	41.0	32.5	41.0
36-37	37.133693772867375	38.0	35.5	41.0	33.0	41.0
38-39	37.27752542895408	38.0	36.0	41.0	33.0	41.0
40-41	37.17898635514655	38.0	36.5	41.0	33.0	41.0
42-43	37.21339927211433	38.0	36.5	41.0	32.5	41.0
44-45	37.052972817060336	38.0	36.0	41.0	33.0	41.0
46-47	36.79063027371784	38.0	36.0	41.0	31.5	41.0
48-49	36.7579133710622	38.0	36.5	40.5	32.0	41.0
50-51	36.36458802525312	38.0	35.0	40.0	30.5	41.0
52-53	36.41908411056549	38.0	35.5	40.0	30.5	41.0
54-55	36.222441706754424	37.5	35.5	40.0	31.0	41.0
56-57	35.926692360870604	37.0	35.0	40.0	29.0	41.0
58-59	35.794564146092284	36.5	35.0	40.0	29.5	41.0
60-61	35.521046659286384	36.5	35.0	40.0	29.0	41.0
62-63	35.33559463014159	37.5	34.5	39.5	28.5	41.0
64-65	35.191725188460865	37.0	35.0	39.0	28.0	41.0
66-67	34.967923450557095	36.5	34.0	39.0	28.0	41.0
68-69	34.49630710462589	36.0	34.0	38.0	27.0	40.0
70-71	34.250014430259974	36.0	34.0	38.0	26.5	40.0
72-73	33.98468273780568	36.0	34.0	37.0	26.0	39.5
74-75	33.70447619991365	35.5	33.0	37.0	26.0	39.0
76-77	32.22212799457088	34.5	31.0	36.0	24.5	37.5
78-79	33.226926181129286	35.0	33.0	37.0	26.0	37.5
80-81	33.22146220072642	35.0	33.0	36.5	26.0	37.0
82-83	33.03750733190665	35.0	33.0	36.0	26.0	37.5
84-85	32.833404150042675	35.0	33.0	36.0	25.0	37.0
86-87	32.58881508047247	35.0	33.0	36.0	25.0	37.0
88-89	32.42454358672536	35.0	33.0	36.0	25.0	36.5
90-91	32.27923262046181	35.0	32.0	35.5	24.0	37.0
92-93	32.12364372238649	35.0	32.0	35.5	24.0	36.5
94-95	31.998593174770633	35.0	32.0	35.0	24.0	36.0
96-97	31.818628147715145	35.0	32.0	35.0	23.0	36.5
98-99	31.682625533252324	35.0	31.5	35.0	22.0	36.5
100	30.493984166418098	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.051439187717782886
1101	2	-0.06634646272540579
1101	3	-0.08988042604655533
1101	4	-0.06606703103180678
1101	5	-0.09180400373504938
1101	6	-0.14046458818835816
1101	7	-0.11860159796484027
1101	8	-0.08141254812649379
1101	9	-0.09414057747158466
1101	10-11	-0.11637058581678872
1101	12-13	-0.10731567536591058
1101	14-15	-0.25790408866824066
1101	16-17	-0.11802168983440708
1101	18-19	-0.11942479906013403
1101	20-21	-0.1942615587542278
1101	22-23	-0.14353266035772805
1101	24-25	-0.26451669592341887
1101	26-27	-0.23487309323808603
1101	28-29	-0.28140418524351674
1101	30-31	-0.3217381289766621
1101	32-33	-0.20524819349572
1101	34-35	-0.1709515621796882
1101	36-37	-0.38158649574128845
1101	38-39	-0.1762476079650952
1101	40-41	-0.2691628738338352
1101	42-43	-0.277398865208788
1101	44-45	-0.3583145688402567
1101	46-47	-0.34303405747348137
1101	48-49	-0.47465950020081493
1101	50-51	-0.3475247138965898
1101	52-53	-0.43869017885135975
1101	54-55	-0.36952282133892567
1101	56-57	-0.36876992354712
1101	58-59	-0.4821424623615016
1101	60-61	-0.4620395386762013
1101	62-63	-0.35717258136289587
1101	64-65	-0.33968652995934434
1101	66-67	-0.4123496012169312
1101	68-69	-0.4717329465492597
1101	70-71	-0.4391614678512852
1101	72-73	-0.5537545224425884
1101	74-75	-0.5332283816523571
1101	76-77	-0.45575326686309126
1101	78-79	-0.43660779097307767
1101	80-81	-0.3339904674324643
1101	82-83	-0.3626008308141522
1101	84-85	-0.37126597656110505
1101	86-87	-0.46816701784835857
1101	88-89	-0.4674304514417251
1101	90-91	-0.41780069253838903
1101	92-93	-0.7782946040844081
1101	94-95	-0.6494454444809712
1101	96-97	-0.5491779637538521
1101	98-99	-0.5640093256267917
1101	100	-0.6171774044247975
1102	1	-0.049015266574848226
1102	2	-0.07029288094753383
1102	3	-0.06539800605815316
1102	4	-0.06627769679080586
1102	5	-0.06749280717193784
1102	6	-0.09441238631720239
1102	7	-0.10583562094488741
1102	8	-0.051236403033897204
1102	9	-0.042277243432970124
1102	10-11	-0.09150679410910101
1102	12-13	-0.08903179635613157
1102	14-15	-0.17812000957199103
1102	16-17	-0.09845610746777567
1102	18-19	-0.08766866077507984
1102	20-21	-0.11691847714654813
1102	22-23	-0.1017545470581851
1102	24-25	-0.10053594102245711
1102	26-27	-0.12496093579993328
1102	28-29	-0.10821479534628509
1102	30-31	-0.10981659737220184
1102	32-33	-0.1495935082524511
1102	34-35	-0.14549820532660362
1102	36-37	-0.19416854269491068
1102	38-39	-0.22398249108699275
1102	40-41	-0.22937539096767523
1102	42-43	-0.19143878361030175
1102	44-45	-0.22859764520428882
1102	46-47	-0.2023035233575854
1102	48-49	-0.22323923121100364
1102	50-51	-0.19835116302862588
1102	52-53	-0.1848937679887399
1102	54-55	-0.25537400529889
1102	56-57	-0.15435099076697867
1102	58-59	-0.37634967145417164
1102	60-61	-0.24363647022362755
1102	62-63	-0.16825104745859676
1102	64-65	-0.24577531085774496
1102	66-67	-0.2698855017900499
1102	68-69	-0.25498036152400516
1102	70-71	-0.24970912982933413
1102	72-73	-0.38041460772082303
1102	74-75	-0.7083041040335232
1102	76-77	-0.3448822467440493
1102	78-79	-0.3131912339323364
1102	80-81	-0.25340247768057367
1102	82-83	-0.20939013285733665
1102	84-85	-0.3179787512775363
1102	86-87	-0.2966177620780499
1102	88-89	-0.2745919948213924
1102	90-91	-0.26040807227951746
1102	92-93	-0.3518843969144143
1102	94-95	-0.4572235761792669
1102	96-97	-0.5086338320371553
1102	98-99	-0.6599661989421577
1102	100	-0.37964187182679865
1103	1	-0.07680409729437798
1103	2	-0.10718011958581997
1103	3	-0.07224163292812591
1103	4	-0.056799573607008824
1103	5	-0.12090029118934353
1103	6	-0.05593912998709527
1103	7	-0.019728920841011188
1103	8	-0.06566827165859479
1103	9	-0.1060746706589768
1103	10-11	-0.06113480700501128
1103	12-13	-0.09049637151893108
1103	14-15	-0.09892824020639779
1103	16-17	-0.08976184060767878
1103	18-19	-0.12475691168232572
1103	20-21	-0.1745397823877326
1103	22-23	-0.07441496217083454
1103	24-25	-0.11664632501651795
1103	26-27	-0.22345731940959013
1103	28-29	-0.14939282219977912
1103	30-31	-0.16212329038287265
1103	32-33	-0.04898649581873826
1103	34-35	-0.05341127581475291
1103	36-37	-0.09457374201396362
1103	38-39	-0.0900346421585212
1103	40-41	-0.17165292175447178
1103	42-43	-0.20879514049460113
1103	44-45	-0.4073852735112098
1103	46-47	-0.3011113215353163
1103	48-49	-0.31856772667364197
1103	50-51	-0.1057080175813212
1103	52-53	-0.1956509847525041
1103	54-55	-0.22983329144791753
1103	56-57	-0.06859406001953516
1103	58-59	-0.12579008052615137
1103	60-61	-0.22487525838392486
1103	62-63	-0.14775620490485863
1103	64-65	-0.2549807174814305
1103	66-67	-0.2545933532812228
1103	68-69	-0.18822543975519324
1103	70-71	-0.2590844587481058
1103	72-73	-0.3759031865171707
1103	74-75	-0.2841342091960044
1103	76-77	-0.17674578034982602
1103	78-79	-0.12946807942402216
1103	80-81	-0.18641851404354526
1103	82-83	-0.1885686596673537
1103	84-85	-0.24240262860628548
1103	86-87	-0.18323159771705377
1103	88-89	-0.25849096356095913
1103	90-91	-0.3556358327946221
1103	92-93	-0.3466304773467783
1103	94-95	-0.01205184626104483
1103	96-97	-0.8072981072260426
1103	98-99	-1.0329397161191523
1103	100	-0.17976714697449836
1104	1	-0.059578211482978816
1104	2	-0.0964324969320316
1104	3	-0.11695152929043218
1104	4	-0.11471468508921845
1104	5	-0.11253025395497218
1104	6	-0.06678147783229349
1104	7	-0.11868286317908883
1104	8	-0.05201343550982074
1104	9	-0.09287798249481227
1104	10-11	-0.13608382115665307
1104	12-13	-0.09943200200851265
1104	14-15	-0.2095112475876988
1104	16-17	-0.06347205412771473
1104	18-19	-0.12424419680937149
1104	20-21	-0.20735124070260014
1104	22-23	-0.11287427635031833
1104	24-25	-0.16004975386582743
1104	26-27	-0.030448367794576825
1104	28-29	-0.15327766664756837
1104	30-31	-0.3171675334635893
1104	32-33	-0.10299402430454307
1104	34-35	-0.06156139920233983
1104	36-37	-0.14550502347470484
1104	38-39	-0.18969074975237987
1104	40-41	-0.15042583935509413
1104	42-43	-0.2441697510477141
1104	44-45	-0.25759671668581063
1104	46-47	-0.12214886912656908
1104	48-49	-0.16019276462984777
1104	50-51	-0.16190418765553005
1104	52-53	-0.15611574042426213
1104	54-55	-0.26293991469746913
1104	56-57	-0.1334712901491315
1104	58-59	-0.1919695991161845
1104	60-61	-0.13902287096295396
1104	62-63	-0.15800961589821583
1104	64-65	-0.23022920225827903
1104	66-67	-0.40788866990003214
1104	68-69	-0.13911432564228932
1104	70-71	-0.21556032985542828
1104	72-73	-0.19544646250602682
1104	74-75	-0.2137163151964785
1104	76-77	-0.3159540595454331
1104	78-79	-0.25621853257684535
1104	80-81	-0.11049804605720226
1104	82-83	-0.08903012838060675
1104	84-85	-0.05837363076560109
1104	86-87	-0.03846844305861907
1104	88-89	-0.09364698293036611
1104	90-91	-0.08806077799556533
1104	92-93	-0.2324893373744068
1104	94-95	-0.29599246487902064
1104	96-97	-0.0016293842403776182
1104	98-99	-0.9618710859450417
1104	100	-0.17542962811599594
1105	1	-0.1136455049538867
1105	2	-1.0596084924049727
1105	3	-0.17802148258872563
1105	4	-0.13547935289684432
1105	5	-0.15957113533916356
1105	6	-0.10046141537151243
1105	7	-0.15304684876053187
1105	8	-0.14827291438525236
1105	9	-0.2276305942004555
1105	10-11	-0.14811136083719845
1105	12-13	-0.14930476566730988
1105	14-15	-0.12974102983907443
1105	16-17	-0.6560777289085493
1105	18-19	-0.14268727281719862
1105	20-21	-0.23250879948454894
1105	22-23	-0.17845796769149302
1105	24-25	-0.2367770007125074
1105	26-27	-0.16352137977091274
1105	28-29	-0.2959587229267129
1105	30-31	-0.21377143712612678
1105	32-33	-0.16130735338290947
1105	34-35	-0.13108643318977897
1105	36-37	-0.20255092688678644
1105	38-39	-0.30872260085109104
1105	40-41	-0.2550034190441437
1105	42-43	-0.22746131360668898
1105	44-45	-0.3911856379509828
1105	46-47	-0.29718901597852465
1105	48-49	-0.2982954665046833
1105	50-51	-0.1947038868957378
1105	52-53	-0.31227926868531597
1105	54-55	-0.3255272457531362
1105	56-57	-0.1636805032844535
1105	58-59	-0.21339507350797504
1105	60-61	-0.2514566168449406
1105	62-63	-0.19591758329989517
1105	64-65	-0.256158752035482
1105	66-67	-0.2987845821362072
1105	68-69	-0.30256856308366764
1105	70-71	-0.30209542943741496
1105	72-73	-0.2983378871827185
1105	74-75	-0.30423294045844784
1105	76-77	-0.10943544352062418
1105	78-79	-0.19968777225169987
1105	80-81	-0.1633591674481849
1105	82-83	-0.05578088145067284
1105	84-85	-0.11530864773399685
1105	86-87	-0.16538566115654874
1105	88-89	-0.13324919303127558
1105	90-91	-0.1196403858210715
1105	92-93	-0.2547828334013502
1105	94-95	-0.29188804892537235
1105	96-97	-0.24452850158428774
1105	98-99	-0.2345451927728277
1105	100	-0.12013113330263181
1106	1	-0.04296792510412928
1106	2	-0.049174209518227485
1106	3	-0.0166686508400673
1106	4	-0.04293156204913373
1106	5	-0.07009326444335073
1106	6	-0.06067903180036893
1106	7	-0.05785271018586258
1106	8	0.036610750707183115
1106	9	-0.10498542610778827
1106	10-11	-0.04484899346775961
1106	12-13	-0.07255454853108745
1106	14-15	-0.15418242602499532
1106	16-17	0.03351299018815723
1106	18-19	-0.07286805960195863
1106	20-21	-0.082720820479949
1106	22-23	0.011094387192606803
1106	24-25	0.05618726367590199
1106	26-27	0.03681001395135297
1106	28-29	0.009523235976075739
1106	30-31	0.04387152306695441
1106	32-33	0.11794953442865364
1106	34-35	0.14716463370070443
1106	36-37	0.039146483346186756
1106	38-39	0.0019420499980427053
1106	40-41	-0.03150095433921507
1106	42-43	0.05094542847305661
1106	44-45	-0.008738935498598721
1106	46-47	-0.0049881211267432946
1106	48-49	0.08226656138061372
1106	50-51	0.22076918299289616
1106	52-53	0.022008994266641935
1106	54-55	0.028427373820022694
1106	56-57	-0.02665397508388878
1106	58-59	-0.0444432568711548
1106	60-61	0.034852370089303975
1106	62-63	0.03712138952049315
1106	64-65	0.15408243098112706
1106	66-67	0.12009794403497409
1106	68-69	0.1432388548325818
1106	70-71	0.06617817577552643
1106	72-73	-0.03576134144422127
1106	74-75	-0.013172793724471887
1106	76-77	0.07613799445566372
1106	78-79	0.09118877432192818
1106	80-81	0.15795142190962963
1106	82-83	0.10364904200880432
1106	84-85	0.16493109466728129
1106	86-87	0.10420122673852461
1106	88-89	0.14756366372979812
1106	90-91	0.1118946837669128
1106	92-93	0.0552649887640797
1106	94-95	0.07361759139682533
1106	96-97	0.015611916653487157
1106	98-99	0.0900557150236132
1106	100	0.14118552647413551
1107	1	-0.07981334796820505
1107	2	-0.09181002897228296
1107	3	-0.060467630360676594
1107	4	-0.03306855891143812
1107	5	-0.07279158373172834
1107	6	-0.13694184080929261
1107	7	-0.12728185715910456
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2316	82-83	-0.0657285944855488
2316	84-85	0.004275729416974627
2316	86-87	-0.23045349620016964
2316	88-89	-0.11646993381860682
2316	90-91	-0.1832984119488401
2316	92-93	-0.29725441885114634
2316	94-95	-0.16989502983791738
2316	96-97	-0.11073335537993856
2316	98-99	-0.03522144683891426
2316	100	-0.2430142362099481
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	3.0
12	23.0
13	104.0
14	489.0
15	1317.0
16	2955.0
17	5771.0
18	9781.0
19	14491.0
20	19774.0
21	27168.0
22	35567.0
23	41134.0
24	44906.0
25	48841.0
26	55673.0
27	65072.0
28	79114.0
29	97397.0
30	120640.0
31	151481.0
32	194062.0
33	260433.0
34	393539.0
35	741128.0
36	1295461.0
37	989383.0
38	1069965.0
39	228530.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30975996091129	18.623554949467035	13.25272956770803	23.813955521913638
2	16.200572071854737	20.764325127671057	39.4522060560549	23.58289674441931
3	18.826668143655393	26.265853299651766	30.026435651393733	24.881042905299108
4	13.113707483685461	17.05573885791711	37.61976702712857	32.21078663126887
5	13.553530314690487	37.12681459648172	34.22953272300287	15.090122365824922
6	30.4458206420973	36.470633572527476	18.765762907191405	14.317782878183818
7	27.28545744849272	32.37206654869997	22.457859046198024	17.884616956609285
8	26.076939797872082	34.542716500262	20.711029419001946	18.66931428286397
9	26.95972171109242	15.994889183907635	20.356332450612626	36.68905665438732
10-11	24.95335396443966	25.694919371110764	28.731672418336178	20.6200542461134
12-13	25.554479077563553	23.60608356963253	28.471246012385848	22.36819134041807
14-15	23.054969679946606	25.078656285774898	26.27738517183338	25.588988862445124
16-17	22.661186339197194	27.738360629592602	26.78101606379005	22.819436967420156
18-19	22.441186185635114	26.966246492524615	28.51075347744706	22.08181384439321
20-21	23.321774088072473	26.235624763109712	28.334856437810625	22.10774471100719
22-23	23.04756084876005	26.038914278597915	28.24334382426156	22.67018104838047
24-25	22.785351041610973	26.11967059020187	28.3025122297504	22.79246613843676
26-27	22.685393265925182	26.18416385632541	28.365527548351384	22.764915329398022
28-29	22.665771195342437	26.513956606289362	28.275741658469393	22.5445305398988
30-31	23.2349764319831	26.168006625605884	28.268481522981737	22.328535419429283
32-33	22.995818182273197	26.311174621129652	28.14948100580516	22.543526190791994
34-35	23.223398270694908	26.37537278423192	28.091081139336723	22.310147805736456
36-37	23.089520684237506	26.318060998971077	28.067075762195188	22.525342554596236
38-39	22.96128188535162	26.524568618412786	27.95620111605091	22.557948380184683
40-41	23.03396270105389	26.266064792354697	28.101925472843252	22.59804703374816
42-43	22.85580967341336	26.44894835779124	27.82268289378944	22.872559075005956
44-45	22.71572719088857	26.515352219166598	27.897205429649258	22.871715160295572
46-47	22.78524531929225	26.37938447969398	27.62483124824491	23.210538952768864
48-49	22.836119517393286	26.44222129635856	27.648796478277145	23.072862707971012
50-51	22.754598818974987	26.712146321299368	27.587524698539127	22.945730161186514
52-53	22.83193069325514	26.767965067008813	27.29374342193127	23.106360817804774
54-55	22.64433146627407	26.530422778401057	27.62099670101889	23.204249054305983
56-57	22.81951076202919	26.68795891761694	27.285636722634944	23.206893597718928
58-59	22.775980571824576	26.598195816909765	27.476672793163903	23.149150818101756
60-61	22.955938853919726	26.62283518819119	27.577979937172287	22.843246020716794
62-63	22.77805809666083	26.703199970037534	27.484659874673127	23.03408205862851
64-65	22.671360141471563	26.489490773276387	27.44433206612294	23.394817019129107
66-67	22.59050282260752	26.54176964483033	27.278115822625953	23.589611709936197
68-69	22.390514419563758	26.610551657173747	27.414738886275135	23.584195036987357
70-71	22.569794834075395	26.4042837893679	27.366678349951012	23.659243026605694
72-73	22.427896363856128	26.690939157196198	27.107278518945982	23.773885960001692
74-75	22.48443580156039	26.838624170904872	26.960665238099367	23.716274789435367
76-77	22.41019592592398	26.60932359246827	27.015284729609913	23.965195751997836
78-79	22.370493937084078	26.563834914648044	27.1168654717176	23.94880567655028
80-81	22.48036765489713	26.71495351346951	26.795229548253786	24.009449283379574
82-83	22.574512686252	26.66390513798649	26.882864444837228	23.878717730924286
84-85	22.533455015993997	26.8138019233501	26.7108120816168	23.941930979039103
86-87	22.31415836858513	26.849649589598844	26.816534559662692	24.019657482153335
88-89	22.207186575654248	26.61556900932198	26.858125509288154	24.319118905735614
90-91	22.597272581603466	26.51767898928253	26.76350515362446	24.121543275489543
92-93	22.511255640331996	26.61428437015792	26.699623377299343	24.174836612210743
94-95	22.342094854110062	26.455183350658185	26.70512166684267	24.497600128389085
96-97	22.216843848535838	26.390732462481676	26.878539741236924	24.513883947745562
98-99	22.24393785569603	26.38478662221329	26.677021770659387	24.69425375143129
100	22.210382717414934	26.362061622036627	26.755117610660022	24.672438049888417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1701.0
1	1231.0
2	1717.5
3	2437.5
4	1662.5
5	1228.0
6	1656.0
7	2895.5
8	4601.5
9	5596.0
10	5136.0
11	4293.0
12	4322.0
13	4779.5
14	5242.5
15	5698.0
16	6192.5
17	6639.0
18	7069.5
19	7630.5
20	8443.5
21	9553.5
22	11145.0
23	13295.0
24	16187.5
25	20232.5
26	25178.5
27	31203.0
28	38683.5
29	47344.5
30	57051.5
31	66831.5
32	77624.0
33	88923.5
34	99399.5
35	110292.0
36	124550.0
37	138479.5
38	149677.0
39	159344.0
40	167083.0
41	175109.0
42	183054.0
43	192424.0
44	202228.0
45	211693.5
46	220694.0
47	229852.5
48	242920.5
49	250116.0
50	248410.5
51	244778.0
52	239930.5
53	234336.0
54	225688.5
55	210826.5
56	190554.0
57	170680.5
58	152786.0
59	132359.5
60	109311.5
61	88571.0
62	72321.0
63	56585.5
64	41977.5
65	31460.5
66	23202.5
67	17729.0
68	13654.5
69	10052.5
70	7535.5
71	5806.0
72	4570.0
73	3594.0
74	2718.0
75	1932.0
76	1294.0
77	781.0
78	478.5
79	305.5
80	203.0
81	142.0
82	86.5
83	49.0
84	36.5
85	35.0
86	24.0
87	13.5
88	9.5
89	6.5
90	8.0
91	5.5
92	2.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030428663801300826
2	0.07575469425532184
3	0.002836004849901941
4	3.336476294002284E-5
5	0.0018183795802312443
6	9.008485993806165E-4
7	1.668238147001142E-5
8	4.170595367502854E-4
9	3.336476294002284E-5
10-11	2.5023572205017127E-5
12-13	9.175309808506279E-5
14-15	0.011802784890033079
16-17	9.842605067306735E-4
18-19	1.3345905176009135E-4
20-21	2.0018857764013702E-4
22-23	0.002026909348606387
24-25	8.59142645705588E-4
26-27	0.006906505928584727
28-29	0.008074272631485526
30-31	0.010543265089047215
32-33	0.0090585331382162
34-35	0.004404148708083014
36-37	0.003845288928837632
38-39	0.0029527815201920208
40-41	0.003069558190482101
42-43	0.0017349676728811872
44-45	0.004937984915123379
46-47	0.0179669248432023
48-49	0.019101326783163074
50-51	0.013771305903494424
52-53	0.007882425244580394
54-55	0.0012178138473108336
56-57	0.0024773336482966954
58-59	0.0027859577054919066
60-61	0.0029694639016620322
62-63	0.0030445346182770834
64-65	0.003511641299437403
66-67	0.002610792700056787
68-69	0.006806411639764659
70-71	4.837890626303311E-4
72-73	0.005330020879668648
74-75	0.007740625002085297
76-77	0.0070816709340198476
78-79	0.004754478718953254
80-81	5.17153825570354E-4
82-83	0.0013929788527459533
84-85	0.0011260607492257705
86-87	0.001518096713771039
88-89	0.0012428374195158506
90-91	0.0011927902751058164
92-93	3.3364762940022835E-4
94-95	0.0011510843214307877
96-97	0.0029611227109270265
98-99	0.003378182247677312
100	0.004854573007773322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5994348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.51359659420531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72714183504523	47.19762450667346
2	14.27480458597454	17.561891415254998
3	4.244738776456388	7.833274462281568
4	1.7116598958192335	4.211614253516153
5	0.8453005429757025	2.5998738300735034
6	0.5314522877249572	1.9614924981708333
7	0.3446477584737398	1.4840366227295516
8	0.23086691991062114	1.1361163662662923
9	0.17614274825461876	0.9751656563216353
>10	0.8450883520738544	9.097659586790037
>50	0.045623519615131176	1.902789508927414
>100	0.021225263856543233	2.3160564650576707
>500	8.257985770491361E-4	0.35712988501931625
>1k	3.7849054747503416E-4	0.5384141138226343
>5k	3.440823158834656E-5	0.17315692527581988
>10k+	6.881646317669313E-5	0.653703903819056
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16826	0.2806977506144121	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14059	0.23453760108689053	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8181	0.1364785628061634	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.6682381470011416E-5	0.0	0.0	0.0	0.0
7	1.6682381470011416E-5	1.6682381470011416E-5	0.0	0.0	3.336476294002283E-5
8	1.6682381470011416E-5	1.6682381470011416E-5	0.0	0.0	3.336476294002283E-5
9	1.6682381470011416E-5	1.6682381470011416E-5	0.0	1.6682381470011416E-5	5.0047144410034255E-5
10-11	1.6682381470011416E-5	1.6682381470011416E-5	0.0	3.336476294002283E-5	5.0047144410034255E-5
12-13	2.5023572205017124E-5	1.6682381470011416E-5	0.0	3.336476294002283E-5	7.507071661505137E-5
14-15	3.336476294002283E-5	1.6682381470011416E-5	0.0	3.336476294002283E-5	1.3345905176009133E-4
16-17	3.336476294002283E-5	1.6682381470011416E-5	0.0	4.170595367502854E-5	1.6682381470011417E-4
18-19	3.336476294002283E-5	1.6682381470011416E-5	0.0	6.672952588004566E-5	1.918473869051313E-4
20-21	3.336476294002283E-5	1.6682381470011416E-5	0.0	1.7516500543511988E-4	2.3355334058015984E-4
22-23	3.336476294002283E-5	1.6682381470011416E-5	0.0	6.422716865954396E-4	2.4189453131516554E-4
24-25	3.336476294002283E-5	2.5023572205017124E-5	0.0	0.0016432145747961246	2.5023572205017125E-4
26-27	3.336476294002283E-5	3.336476294002283E-5	0.0	0.0029360991387220093	2.5023572205017125E-4
28-29	3.336476294002283E-5	3.336476294002283E-5	0.0	0.006906505928584727	2.5023572205017125E-4
30-31	3.336476294002283E-5	3.336476294002283E-5	0.0	0.019618480608733425	2.5023572205017125E-4
32-33	3.336476294002283E-5	3.336476294002283E-5	0.0	0.050639368952219654	2.836004849901941E-4
34-35	3.336476294002283E-5	4.170595367502854E-5	0.0	0.0989098397356977	3.1696524793021694E-4
36-37	3.336476294002283E-5	5.0047144410034255E-5	0.0	0.1713864460321623	3.1696524793021694E-4
38-39	3.336476294002283E-5	5.0047144410034255E-5	0.0	0.274708775666678	3.1696524793021694E-4
40-41	3.336476294002283E-5	5.838833514503996E-5	0.0	0.393061931005674	3.1696524793021694E-4
42-43	3.336476294002283E-5	6.672952588004566E-5	0.0	0.5124410528050757	3.1696524793021694E-4
44-45	4.170595367502854E-5	6.672952588004566E-5	0.0	0.6607057181198022	3.3364762940022835E-4
46-47	5.0047144410034255E-5	6.672952588004566E-5	0.0	0.8364879716693125	3.3364762940022835E-4
48-49	5.0047144410034255E-5	6.672952588004566E-5	0.0	1.018275882548027	3.41988820135234E-4
50-51	5.0047144410034255E-5	6.672952588004566E-5	0.0	1.2073623353198712	3.6701239234025116E-4
52-53	5.0047144410034255E-5	6.672952588004566E-5	0.0	1.4017954913528543	3.836947738102626E-4
54-55	5.0047144410034255E-5	6.672952588004566E-5	0.0	1.6307695182194961	4.0871834601527977E-4
56-57	5.0047144410034255E-5	6.672952588004566E-5	0.0	1.8868023678304962	4.254007274852911E-4
58-59	5.0047144410034255E-5	6.672952588004566E-5	0.0	2.163621464753131	4.3374191822029686E-4
60-61	5.0047144410034255E-5	6.672952588004566E-5	0.0	2.4533277013613493	4.5042429969030826E-4
62-63	5.0047144410034255E-5	6.672952588004566E-5	0.0	2.7527597663665837	5.004714441003425E-4
64-65	5.0047144410034255E-5	6.672952588004566E-5	0.0	3.0586228894285084	5.004714441003425E-4
66-67	5.0047144410034255E-5	6.672952588004566E-5	0.0	3.4022132181848637	5.254950163053596E-4
68-69	5.0047144410034255E-5	6.672952588004566E-5	0.0	3.790178681651449	5.505185885103768E-4
70-71	5.0047144410034255E-5	6.672952588004566E-5	0.0	4.203159376132317	5.505185885103768E-4
72-73	5.0047144410034255E-5	6.672952588004566E-5	0.0	4.605254816704002	6.339304958604339E-4
74-75	5.0047144410034255E-5	6.672952588004566E-5	0.0	5.006741350352032	6.83977640270468E-4
76-77	5.0047144410034255E-5	6.672952588004566E-5	0.0	5.447514892361938	7.090012124754852E-4
78-79	5.0047144410034255E-5	6.672952588004566E-5	0.0	5.942297644381007	7.17342403210491E-4
80-81	5.0047144410034255E-5	6.672952588004566E-5	0.0	6.445221398557441	7.590483568855195E-4
82-83	5.0047144410034255E-5	6.672952588004566E-5	0.0	6.9592973247465775	8.341190735005709E-4
84-85	5.0047144410034255E-5	6.672952588004566E-5	0.0	7.482256619068496	8.341190735005709E-4
86-87	6.672952588004566E-5	6.672952588004566E-5	0.0	8.014049234378785	8.424602642355765E-4
88	8.341190735005709E-5	6.672952588004566E-5	0.0	8.43137568923259	8.508014549705822E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	6660	0.0	42.23045	2
GTACCTG	8285	0.0	34.981274	1
ACCTGGG	8030	0.0	34.237213	3
GGTATCA	14590	0.0	33.408108	1
GTATCAA	21650	0.0	31.010838	1
TCAACGC	25395	0.0	25.962673	4
ATCAACG	25735	0.0	25.529428	3
GTACATG	19695	0.0	25.513067	1
CAACGCA	25980	0.0	25.486595	5
TATCAAC	26405	0.0	25.043665	2
AACGCAG	26970	0.0	24.742302	6
TACATGG	20235	0.0	24.312366	2
ACATGGG	20740	0.0	23.044659	3
CCTGGGG	8320	0.0	22.649626	4
TATCACG	610	0.0	22.359749	2
ACGCAGA	29820	0.0	22.330133	7
CGCAGAG	30655	0.0	21.757996	8
CATGGGG	14770	0.0	21.412853	4
ATGGGGG	8625	0.0	20.105146	5
GAGTACT	17445	0.0	19.93911	12-13
>>END_MODULE