FastQCFastQC Report
Mon 6 Jun 2016
ERR522942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647057
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228310.3434753154666795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183290.2757460933462734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.16413278839041098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98200.049.426162
ACCTGGG109900.042.7951163
TATAACG6650.038.1909752
GTACCTG143250.034.8016551
ATAACGC7900.033.339023
GGTATCA179850.033.3155981
GTATCAA267100.030.95521
TAACGCA8800.029.9288964
CCTGGGG105200.029.7297524
TCAACGC311550.026.2214954
CAACGCA315350.025.8741345
ATCAACG315450.025.7784233
TATCAAC320400.025.5561182
AACGCAG324900.025.3200386
GTACATG260050.024.433581
TACATGG262350.023.645712
CATGGGG155700.023.0473634
ATGGGGG96750.022.8943945
ACGCAGA360750.022.776057
ACATGGG262150.022.5157153
Produced by FastQC (version 0.11.5)