FastQCFastQC Report
Mon 6 Jun 2016
ERR522942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647057
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT217360.3270018596199792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210550.31675672406600397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179210.2696080385650371No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT116020.17454341071544896TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.15946907029682458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG103050.051.954472
ACCTGGG114400.045.441243
GTACCTG145450.037.9025421
ATAACGC7200.036.557513
TATAACG7150.035.5005382
CCTGGGG100400.033.8006134
GGTATCA184000.032.211221
TAACGCA8400.030.2159024
GTATCAA268550.029.4264981
CTGGGGG60450.025.1924135
TATCACG5250.025.0695152
TCAACGC314450.024.7381634
CAACGCA317600.024.5224085
ATCAACG319000.024.3558463
TATCAAC323200.024.1425952
AACGCAG327600.023.7855246
GTACATG257000.023.006821
CATGGGG148700.022.4107464
TGGGGGG95650.022.3562266
TACATGG259700.022.226632
Produced by FastQC (version 0.11.5)