FastQCFastQC Report
Mon 6 Jun 2016
ERR522941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7575341
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282150.3724584807469393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228590.30175539292554615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145170.19163493762194994No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112590.14862697269997482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG151450.057.692152
GTACCTG169450.051.9938051
ACCTGGG176900.049.0472453
CCTGGGG149800.037.5720674
TATAACG10900.030.6317752
ATAACGC11600.028.3782833
TAACGCA12650.026.762384
CTGGGGG90250.026.568585
GTACCCG17150.025.7821521
GTATCAA396800.023.8868471
GGTATCA296450.023.1028391
GTACATG335400.022.4394571
CATGGGG185500.022.3059924
GTATAAC21300.022.3047431
TACATGG342050.021.5711692
TATCACG7650.020.9005552
ACATGGG346600.020.7184163
TCAACGC450550.020.6426774
CAACGCA453500.020.5273675
TACCCGG19500.020.498622
Produced by FastQC (version 0.11.5)