FastQCFastQC Report
Mon 6 Jun 2016
ERR522941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7575341
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249830.3297937346978836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223950.2956302561165233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141910.18733150098457613No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100420.13256168930217133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG159650.058.4639662
GTACCTG180700.052.1186141
ACCTGGG181400.050.6206783
CCTGGGG151650.041.121524
TATAACG9550.031.4959352
CTGGGGG87200.031.1491575
ATAACGC9800.028.771723
TAACGCA11900.026.0637954
GGTATCA301100.023.7045141
TGGGGGG108050.023.5292156
GTATCAA398300.022.5118121
GTATAAC21300.022.0744781
TATCACG7600.021.6437452
GTACCCG17550.021.4329991
CATGGGG184400.020.9484334
GTACATG337050.020.8274231
TACCCGG16050.020.4975012
GTACCGT9450.020.388586
TACATGG339250.020.3230172
GAGTACT247600.020.10497512-13
Produced by FastQC (version 0.11.5)