##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522941_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7575341
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.217931047592444	33.0	31.0	34.0	30.0	34.0
2	32.3472032480122	34.0	31.0	34.0	30.0	34.0
3	32.543399960477025	34.0	31.0	34.0	30.0	35.0
4	35.63390611722957	37.0	35.0	37.0	33.0	37.0
5	35.56824214249893	36.0	35.0	37.0	33.0	37.0
6	35.686482364292246	37.0	35.0	37.0	33.0	37.0
7	35.52979067212948	36.0	35.0	37.0	33.0	37.0
8	35.51563804190465	36.0	35.0	37.0	33.0	37.0
9	36.80587395339695	38.0	37.0	39.0	33.0	39.0
10-11	36.60492253748049	37.5	35.0	39.0	33.0	39.0
12-13	36.61205211752184	37.5	35.0	39.0	33.0	39.0
14-15	37.78468936249867	38.0	37.0	41.0	33.0	41.0
16-17	37.59959353644938	38.0	36.5	41.0	33.0	41.0
18-19	37.50228365957388	38.0	36.0	41.0	32.0	41.0
20-21	37.51773359113471	38.0	36.5	41.0	33.0	41.0
22-23	37.37170940555679	38.0	36.0	41.0	33.0	41.0
24-25	37.29715692006472	38.0	36.5	40.5	32.0	41.0
26-27	36.98389089019227	38.0	36.0	40.0	32.5	41.0
28-29	36.946868530406746	38.0	36.0	40.0	32.0	41.0
30-31	36.705529625663054	38.0	35.5	40.0	31.5	41.0
32-33	36.660177277828154	37.5	36.0	40.0	30.5	41.0
34-35	36.429733526187135	38.0	35.0	40.0	30.5	41.0
36-37	36.206275796693504	37.0	35.5	40.0	30.5	41.0
38-39	36.15871278929886	37.0	35.0	40.0	30.0	41.0
40-41	36.35231984936388	37.5	35.5	40.0	30.5	41.0
42-43	36.39601042382118	37.5	35.5	40.0	30.5	41.0
44-45	36.218365153463054	37.0	35.0	40.0	30.5	41.0
46-47	36.11460335580933	37.0	35.5	40.0	30.0	41.0
48-49	35.89778677949943	38.0	35.0	40.0	29.5	41.0
50-51	35.735054963202316	38.0	34.5	40.0	28.5	41.0
52-53	35.7470305957184	37.5	35.0	40.0	28.5	41.0
54-55	35.55198327045608	37.5	34.0	40.0	28.0	41.0
56-57	35.306829883961655	37.0	35.0	40.0	27.5	41.0
58-59	35.123001987633295	37.0	34.0	40.0	27.5	41.0
60-61	34.93734697883567	37.0	34.0	39.0	26.5	41.0
62-63	34.69519517339219	36.5	34.0	39.0	26.0	41.0
64-65	34.50961804887727	37.0	33.5	39.0	26.0	40.5
66-67	34.01073865849735	36.0	33.5	38.0	26.0	40.0
68-69	33.871772703037394	36.5	33.0	37.5	25.5	40.0
70-71	33.454633263373886	35.0	33.0	37.0	25.5	39.5
72-73	33.22354129008846	35.0	33.0	37.0	25.0	39.0
74-75	33.03404262593591	35.0	32.5	37.0	24.0	39.0
76-77	31.43582975340648	34.0	30.0	36.0	23.0	37.0
78-79	32.55344373276398	35.0	32.0	36.5	23.5	37.0
80-81	32.66151662347609	35.0	32.0	36.0	24.0	37.5
82-83	32.55888830615018	35.0	32.0	37.0	24.5	37.0
84-85	32.22427313833133	35.0	32.0	36.0	24.0	36.5
86-87	32.099243057177226	35.0	32.0	36.0	24.0	36.5
88-89	32.05666497389359	35.0	32.0	36.0	23.0	37.0
90-91	31.81950211878251	34.5	32.0	35.0	23.0	36.5
92-93	31.60228476843485	35.0	31.0	35.0	21.5	36.5
94-95	31.73660855663131	34.5	31.0	35.0	21.0	37.0
96-97	31.463152615836037	34.5	31.0	35.0	21.0	37.0
98-99	31.312506790123376	34.0	31.0	35.0	20.5	36.0
100	30.079275111179815	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09539084320141455
1101	2	-0.1472757535930853
1101	3	-0.11392711751130236
1101	4	-0.12239754415323034
1101	5	-0.17643166312280556
1101	6	-0.0780242362595871
1101	7	-0.04946234817740702
1101	8	-0.19221032652683334
1101	9	-0.09790258370333049
1101	10-11	-0.1846730619639132
1101	12-13	-0.12209549748608595
1101	14-15	-0.3141470914863973
1101	16-17	-0.20794690343281985
1101	18-19	-0.35100101479677903
1101	20-21	-0.3336245072726882
1101	22-23	-0.830749229346246
1101	24-25	-0.3096846864405194
1101	26-27	-0.2867315159334254
1101	28-29	-0.34377427324685783
1101	30-31	-0.32993278891114386
1101	32-33	-0.31197488007668284
1101	34-35	-0.406555219788018
1101	36-37	-0.4112339147267079
1101	38-39	-0.3141681912416132
1101	40-41	-0.3377197989741916
1101	42-43	-0.3494246476156704
1101	44-45	-0.41975474719272654
1101	46-47	-0.33888782130372874
1101	48-49	-0.345806236052411
1101	50-51	-0.30299693105428815
1101	52-53	-0.2943833410381913
1101	54-55	-0.2542897194997735
1101	56-57	-0.355689462239674
1101	58-59	-0.41762372788728896
1101	60-61	-0.6381219169340397
1101	62-63	-0.4620180506044633
1101	64-65	-0.4540448971158
1101	66-67	-0.3707722708024761
1101	68-69	-0.3852427531197975
1101	70-71	-0.5397870996329672
1101	72-73	-0.44330893311396835
1101	74-75	-0.5218902658104767
1101	76-77	-0.6388995766241727
1101	78-79	-0.5211143765832418
1101	80-81	-0.4008792195148345
1101	82-83	-0.3164527915716562
1101	84-85	-0.40872739692916227
1101	86-87	-0.31507382786573856
1101	88-89	-0.37526187709181613
1101	90-91	-0.3985593777779357
1101	92-93	-0.3660552980579368
1101	94-95	-0.5374922496111552
1101	96-97	-0.5979218340334356
1101	98-99	-0.5436683044271469
1101	100	-0.5334012738007488
1102	1	-0.01891284754543676
1102	2	-0.12060796516511374
1102	3	-0.12118693881592435
1102	4	-0.1072637335050004
1102	5	-0.17503390780293415
1102	6	-0.17237208693785533
1102	7	-0.19708196870661965
1102	8	-0.1791180047022607
1102	9	-0.19388940812500977
1102	10-11	-0.25141879404841916
1102	12-13	-0.20333044043014326
1102	14-15	-0.30200908958422445
1102	16-17	-0.319179000491296
1102	18-19	-0.3334211789132411
1102	20-21	-0.35442084962042486
1102	22-23	-0.3436304012162026
1102	24-25	-0.2714609184597023
1102	26-27	-0.3710049484551945
1102	28-29	-0.33480652937387845
1102	30-31	-0.4706127782072187
1102	32-33	-0.29834038500749926
1102	34-35	-0.35483345715424974
1102	36-37	-0.47730640373197275
1102	38-39	-0.47263302786251415
1102	40-41	-0.4313901776125988
1102	42-43	-0.4205774689881707
1102	44-45	-0.277425845164295
1102	46-47	-0.30787100330485373
1102	48-49	-0.31299042379352215
1102	50-51	-0.21522681876961514
1102	52-53	-0.34018106571578954
1102	54-55	-0.28877271099273827
1102	56-57	-0.27163343927943373
1102	58-59	-0.3233988617180543
1102	60-61	-0.35117690653750344
1102	62-63	-0.47953699504396496
1102	64-65	-0.48388057759058256
1102	66-67	-0.5759543422748123
1102	68-69	-0.45615784915297297
1102	70-71	-0.42870752460364514
1102	72-73	-0.49342839473114
1102	74-75	-0.45160512655694873
1102	76-77	-0.4490140655798065
1102	78-79	-0.5080483506966331
1102	80-81	-0.435396955552136
1102	82-83	-0.36789750139527655
1102	84-85	-0.4521113352100343
1102	86-87	-0.45881782022663486
1102	88-89	-0.4626990565338076
1102	90-91	-0.5891370131860896
1102	92-93	-0.48217844889367356
1102	94-95	-0.6755530777884431
1102	96-97	-0.6999529330274044
1102	98-99	-0.7208129538083092
1102	100	-0.8034637797017297
1103	1	-0.12550810919880462
1103	2	-0.17801971473613776
1103	3	-0.2637682082902302
1103	4	-0.1266649794824346
1103	5	-0.17977119499260397
1103	6	-0.21989191833550592
1103	7	-0.17909382687027176
1103	8	-0.13696073836086953
1103	9	-0.1260379340841311
1103	10-11	-0.23729075123189602
1103	12-13	-0.2405978034265459
1103	14-15	-0.28808822409720136
1103	16-17	-0.3504559854756266
1103	18-19	-0.38240469326582627
1103	20-21	-0.3536861750861533
1103	22-23	-0.4043048673812706
1103	24-25	-0.37790515311814943
1103	26-27	-0.387202607848927
1103	28-29	-0.34145693867727545
1103	30-31	-0.4904835075750853
1103	32-33	-0.3321594496352489
1103	34-35	-0.45961899617287116
1103	36-37	-0.3921188638847184
1103	38-39	-0.3169714168700679
1103	40-41	-0.4197811526949451
1103	42-43	-0.4977229017600635
1103	44-45	-0.41581232708475824
1103	46-47	-0.4287053692771394
1103	48-49	-0.45192906328303195
1103	50-51	-0.4339072764478047
1103	52-53	-0.43952283168922435
1103	54-55	-1.2297656619312036
1103	56-57	-0.4671191358897957
1103	58-59	-0.4594652072734178
1103	60-61	-0.4626831562892022
1103	62-63	-0.46855542362918357
1103	64-65	-0.4658762887493282
1103	66-67	-0.31203144304082997
1103	68-69	-0.40118450542703243
1103	70-71	-0.43450309803515097
1103	72-73	-0.5087660818595126
1103	74-75	-0.6557173818215176
1103	76-77	-0.4284387516733261
1103	78-79	-0.4376330974052749
1103	80-81	-1.266531266092997
1103	82-83	-0.29418806045813284
1103	84-85	-0.26945866702862276
1103	86-87	-0.2763470352381461
1103	88-89	-0.3917576776344518
1103	90-91	-0.3829079678742069
1103	92-93	-0.32794279721493425
1103	94-95	-0.4032983996974835
1103	96-97	-0.5345701534723304
1103	98-99	-0.45814784666294983
1103	100	-0.5206878569954654
1104	1	-0.0484820478087542
1104	2	-0.16570167603362762
1104	3	-0.13072200522504573
1104	4	-0.05957401338420709
1104	5	-0.054987002677904684
1104	6	-0.24531287897024612
1104	7	-0.17815082172783292
1104	8	-0.06623443270981966
1104	9	-0.12267207016810744
1104	10-11	-0.20176209797173073
1104	12-13	-0.15193478972276608
1104	14-15	-0.33671030859535733
1104	16-17	-0.34165688216317847
1104	18-19	-0.2988021721769485
1104	20-21	-0.38339706094060944
1104	22-23	-0.3190521201491521
1104	24-25	-0.2385925103118396
1104	26-27	-0.2617847732292091
1104	28-29	-0.3999345224272588
1104	30-31	-0.4234590138862657
1104	32-33	-0.27142730500119683
1104	34-35	-0.3347511899970641
1104	36-37	-0.4501228798283421
1104	38-39	-0.33464460091819603
1104	40-41	-0.25854804772654916
1104	42-43	-0.25848278625709753
1104	44-45	-0.4000129872090099
1104	46-47	-0.38275177511086866
1104	48-49	-0.3715524113295956
1104	50-51	-0.36888274708673663
1104	52-53	-0.3775005064972916
1104	54-55	-0.3777122267880273
1104	56-57	-0.3181496412903826
1104	58-59	-0.48643117970816974
1104	60-61	-0.573279652523901
1104	62-63	-0.5215747974123346
1104	64-65	-0.6142506670666066
1104	66-67	-0.5452586076419408
1104	68-69	-0.32704623417905765
1104	70-71	-0.3515654887888999
1104	72-73	-0.4272630882072974
1104	74-75	-0.48639697560853534
1104	76-77	-0.35571147723925023
1104	78-79	-0.3489745333444958
1104	80-81	-0.37810571688125805
1104	82-83	-0.3704477506353072
1104	84-85	-1.0243005357284787
1104	86-87	-0.37466167193512234
1104	88-89	-0.4452360123790271
1104	90-91	-0.40629902765900994
1104	92-93	-0.35421839909016484
1104	94-95	-0.35980346797227725
1104	96-97	-0.3305001664082141
1104	98-99	-0.3065118894170702
1104	100	-1.6067469752902568
1105	1	-0.01595283357811894
1105	2	-0.15093633489463087
1105	3	-0.15084106399798003
1105	4	-0.06491956784529407
1105	5	-0.13821808798129354
1105	6	-0.10023825800989528
1105	7	-0.13668826502503606
1105	8	-0.18953261341069805
1105	9	-0.07510750498649799
1105	10-11	-0.12448909926181528
1105	12-13	-0.1245874068580477
1105	14-15	-0.2205087748601997
1105	16-17	-0.16851348551548995
1105	18-19	-0.2347879311909722
1105	20-21	-0.27568528212935917
1105	22-23	-0.2747701616583811
1105	24-25	-0.13413671595201748
1105	26-27	-0.2850674843498169
1105	28-29	-0.2887614847841462
1105	30-31	-0.32535155726176157
1105	32-33	-0.20939351593728617
1105	34-35	-0.37433428646355793
1105	36-37	-0.3698861529635451
1105	38-39	-0.3172179367303869
1105	40-41	-0.2644486060140352
1105	42-43	-0.30264325320234775
1105	44-45	-0.46018000204848164
1105	46-47	-0.37745602703549963
1105	48-49	-0.3330127301463506
1105	50-51	-0.25079005992173364
1105	52-53	-0.440010609342977
1105	54-55	-1.304730023387357
1105	56-57	-0.3553448626131157
1105	58-59	-0.35683369546566013
1105	60-61	-0.2854219823699182
1105	62-63	-0.32586092647547815
1105	64-65	-0.3140863887285974
1105	66-67	-0.4229054245119741
1105	68-69	-0.1455281024483881
1105	70-71	-0.17693863771591367
1105	72-73	-0.29901164498039634
1105	74-75	-0.25629014982815335
1105	76-77	-0.2886162268379664
1105	78-79	-0.24653507945046726
1105	80-81	-0.23331024333854344
1105	82-83	-0.13666378319504702
1105	84-85	-0.21028497098473764
1105	86-87	-0.18895000431951559
1105	88-89	-0.30402817976319696
1105	90-91	-0.28010615644755177
1105	92-93	-0.1999737955725287
1105	94-95	-0.24801081976987405
1105	96-97	-0.32870550021064915
1105	98-99	-0.2089918502985597
1105	100	-0.1819389532594009
1106	1	-0.04854665430220706
1106	2	-0.138467587455672
1106	3	-0.13579570601982738
1106	4	-0.07375438159074577
1106	5	-0.1573807778134153
1106	6	-0.15950635234440114
1106	7	-0.09008119308898443
1106	8	-0.20081202990347435
1106	9	-0.1892169567191786
1106	10-11	-0.1896936987310056
1106	12-13	-0.21866445553198588
1106	14-15	-0.19570826801079733
1106	16-17	-0.1580654533393826
1106	18-19	-0.22713103797607204
1106	20-21	-0.2560168145923001
1106	22-23	-0.2424855568937403
1106	24-25	-0.13331560731351288
1106	26-27	-0.1528327449927076
1106	28-29	-0.11266088400369512
1106	30-31	-0.3351415677895986
1106	32-33	-0.13814158530757226
1106	34-35	-0.15971785924751458
1106	36-37	-0.2891384870554674
1106	38-39	-0.3041615213660407
1106	40-41	-0.2050098597728507
1106	42-43	-0.22563309730202974
1106	44-45	-0.21074547955230827
1106	46-47	-0.32041466585486944
1106	48-49	-0.23094549064740733
1106	50-51	-0.1378408772479105
1106	52-53	-0.26017003995693955
1106	54-55	-0.28262268983127825
1106	56-57	-0.1396276139732251
1106	58-59	-0.19774797865830607
1106	60-61	-0.23191696746072665
1106	62-63	-0.2869685563241191
1106	64-65	-0.19483443288249447
1106	66-67	-0.18789574772303297
1106	68-69	-0.08413729256104574
1106	70-71	-0.1274962350984552
1106	72-73	-0.17734969587967697
1106	74-75	-0.12120520212064179
1106	76-77	-0.28023196690743646
1106	78-79	-0.0628142122588784
1106	80-81	-0.001205305339340157
1106	82-83	-0.054981724024791845
1106	84-85	0.03132396065993959
1106	86-87	0.11110011325189362
1106	88-89	0.010305571741909603
1106	90-91	-0.06748831711376724
1106	92-93	0.024210233628050304
1106	94-95	0.0814001254033947
1106	96-97	0.14227680418898103
1106	98-99	-0.08371153862400504
1106	100	0.07318079306373804
1107	1	0.014105597389480806
1107	2	-0.08586478075782367
1107	3	-0.049365412349899884
1107	4	0.009985215515889934
1107	5	-0.07797767333448036
1107	6	-0.09806344742323603
1107	7	-0.18990541335809752
1107	8	-0.10599986532387362
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2316	94-95	0.2861912249217795
2316	96-97	0.26358702491728536
2316	98-99	0.32970400226885843
2316	100	0.2608715471493177
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	4.0
11	7.0
12	45.0
13	251.0
14	950.0
15	2495.0
16	5761.0
17	10636.0
18	17725.0
19	25286.0
20	33744.0
21	43977.0
22	55670.0
23	64692.0
24	72015.0
25	80770.0
26	92795.0
27	110784.0
28	132634.0
29	162708.0
30	199906.0
31	246424.0
32	308052.0
33	399432.0
34	565119.0
35	1002153.0
36	1534140.0
37	1121688.0
38	1073953.0
39	211399.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62462774576703	17.8923047869088	12.720562696527123	23.762504770797044
2	16.644936088865652	20.30583873957368	39.25194532238091	23.797279849179755
3	19.355419115786336	26.053929453472787	29.437156690372092	25.15349474036878
4	13.216933410073766	16.38256865716564	36.443371369130276	33.95712656363031
5	13.683981249612225	36.1915749982509	33.59960186447815	16.524841887658724
6	30.830229292648344	35.02362467907385	19.164615823894923	14.981530204382878
7	28.322130977338183	31.558790554775022	21.77957401521595	18.339504452670845
8	27.04370418851278	33.80290093986565	20.340174920247783	18.813219951373785
9	27.417115612353292	15.886202350494848	20.209651288305043	36.48703074884681
10-11	25.578150579502	25.37139419673611	27.962875068124	21.087580155637887
12-13	25.951116147052993	23.25272313453016	28.05032682762657	22.745833890790273
14-15	23.40295348358087	24.603911472198202	26.275988570201132	25.7171464740198
16-17	22.889414432848156	27.08551038781712	26.88028862528085	23.144786554053876
18-19	22.568789839396594	26.303323119266963	28.697302265410617	22.430584775925823
20-21	23.473472469206094	25.62340591721944	28.572121615026557	22.33099999854791
22-23	23.2025999272107	25.49467089638428	28.332635230154256	22.970093946250763
24-25	22.9251382097078	25.581715097407688	28.434087558502537	23.059059134381982
26-27	22.882464529062773	25.542831031273995	28.55119982762281	23.02350461204042
28-29	22.834867875843653	25.92360033919729	28.47931697928523	22.762214805673832
30-31	23.417970393806403	25.619130257416316	28.456073078470506	22.506826270306775
32-33	23.061054147687873	25.802832222796862	28.373318477040787	22.762795152474474
34-35	23.281495359626398	25.8920747526671	28.23715706296765	22.589272824738853
36-37	23.38149220124955	25.83593967152449	28.136518413587076	22.646049713638885
38-39	23.278268854037975	26.096535478381366	27.9598155967334	22.665380070847256
40-41	23.275117552365558	25.86485378991361	28.12119385519745	22.738834802523378
42-43	23.05534909694343	25.947922263601956	27.887437737876624	23.10929090157799
44-45	23.026160235950254	26.06124345974299	27.851418720742693	23.061177583564067
46-47	23.06500101000899	25.876179499061948	27.70620356764222	23.352615923286844
48-49	23.05657204632487	25.95920405471992	27.752605364571476	23.231618534383735
50-51	22.893565332774404	26.361561985803426	27.645207873633694	23.099664807788475
52-53	23.039818717540665	26.45998839788036	27.18613744735941	23.31405543721957
54-55	22.868327141398876	26.203054306295968	27.513165767716814	23.415452784588336
56-57	23.05195598126322	26.320021512737807	27.221177637577693	23.406844868421278
58-59	23.039624883607853	26.28061725694356	27.408556666093258	23.271201193355324
60-61	23.288647386201458	26.313176363333703	27.46186451697475	22.936311733490093
62-63	23.03771025081094	26.351870595693715	27.461089966077374	23.14932918741797
64-65	22.966991246843037	26.224733093924428	27.314745877536062	23.493529781696466
66-67	22.93780136300083	26.233017025596645	27.205180329864127	23.624001281538394
68-69	22.755996019979307	26.276156012850915	27.248680339324814	23.719167627844964
70-71	22.827695941554467	26.161393835632037	27.2437696249792	23.7671405978343
72-73	22.786938515690494	26.32800850522134	27.040895701667228	23.844157277420933
74-75	22.7364513687181	26.50862097417021	26.915497740035576	23.83942991707611
76-77	22.65248273015101	26.18389242270565	27.14396361565891	24.019661231484427
78-79	22.58500390227726	26.262279764236972	27.11571809822333	24.036998235262434
80-81	22.666275132878592	26.434563430585783	26.79393362881858	24.105227807717043
82-83	22.71560047219455	26.40210395210466	26.871103891710757	24.011191683990038
84-85	22.697123381866824	26.49852765276287	26.690143345778573	24.11420561959173
86-87	22.511261250855245	26.393800493788177	26.89907994560753	24.19585830974905
88-89	22.394803709120655	26.223991797581263	26.954917388709255	24.426287104588827
90-91	22.772672924498444	26.22033495009938	26.833590583616573	24.173401541785598
92-93	22.60850521642432	26.26775110279403	26.825194078019837	24.298549602761817
94-95	22.35067484434196	26.110321524203634	26.89534904673249	24.643654584721915
96-97	22.21948888897836	26.09961567369114	27.054362301950043	24.626533135380456
98-99	22.32505560500716	26.01389620249054	26.80351436582042	24.857533826681877
100	22.294420068522463	25.917709680085053	26.940134325572256	24.84773592582022
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2914.0
1	2152.0
2	4305.5
3	6102.5
4	3596.0
5	2393.5
6	3246.0
7	5890.5
8	8941.5
9	10300.5
10	9169.5
11	7360.0
12	7309.5
13	7931.5
14	8531.0
15	9076.0
16	9631.0
17	10452.5
18	11381.5
19	12318.0
20	13211.5
21	14279.5
22	15986.5
23	18496.5
24	21383.0
25	25000.5
26	30618.5
27	37398.0
28	44678.5
29	53813.0
30	64076.0
31	74615.5
32	86411.5
33	98677.5
34	112360.5
35	125959.0
36	142160.0
37	158997.5
38	171465.5
39	182912.5
40	193275.0
41	202821.0
42	213232.5
43	225418.5
44	237895.5
45	250906.0
46	265214.5
47	281942.5
48	304585.0
49	317530.0
50	324650.0
51	323454.5
52	309479.5
53	302366.5
54	294712.0
55	279563.5
56	257289.0
57	233765.0
58	213153.0
59	188258.0
60	157629.0
61	128601.5
62	104509.5
63	82047.0
64	61134.5
65	45360.5
66	33807.0
67	26030.0
68	20163.0
69	15227.0
70	11818.0
71	9116.5
72	7194.0
73	5740.5
74	4492.0
75	3148.0
76	2093.0
77	1325.0
78	809.0
79	522.5
80	350.5
81	199.0
82	123.0
83	105.5
84	77.5
85	50.5
86	32.5
87	24.0
88	19.5
89	13.0
90	7.0
91	6.0
92	3.5
93	0.5
94	1.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04265154532317423
2	0.010045752395832743
3	0.0
4	2.772152435118102E-4
5	4.0922250232695796E-4
6	0.0
7	0.0
8	3.960217764454432E-5
9	0.0
10-11	6.6003629407573866E-6
12-13	2.310127029265085E-4
14-15	0.012270074706867981
16-17	0.0015972878316632877
18-19	4.6202540585301704E-5
20-21	7.920435528908864E-4
22-23	6.864377458387682E-4
24-25	0.004600452969707898
26-27	0.01654050952953801
28-29	0.013042317170936594
30-31	0.009814739692906234
32-33	0.007240598146010854
34-35	0.008996294688252316
36-37	0.004983274020271826
38-39	0.004125226837973366
40-41	0.00553110414435469
42-43	0.006745570925454049
44-45	0.006917180361913741
46-47	0.015194035489623503
48-49	0.017860582117689486
50-51	0.017537164333592373
52-53	0.01139222643574725
54-55	0.00394041667563216
56-57	0.003881013409165343
58-59	0.009662931345268813
60-61	0.0023497292069096297
62-63	1.7820979940044943E-4
64-65	2.2441233998575112E-4
66-67	9.306511746467915E-4
68-69	4.6202540585301704E-5
70-71	1.914105252819642E-4
72-73	0.0030295665898076403
74-75	0.005847921565511044
76-77	0.005729115032577411
78-79	0.003656601069179592
80-81	6.600362940757386E-5
82-83	9.702533522913358E-4
84-85	2.9041596939332503E-4
86-87	3.6962032468241363E-4
88-89	1.914105252819642E-4
90-91	4.422243170307449E-4
92-93	4.884268576160466E-4
94-95	1.980108882227216E-5
96-97	0.001815099808708281
98-99	4.4882467997150224E-4
100	1.980108882227216E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7575341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.4954333601366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48155297601095	39.668997596592895
2	16.95437200432106	18.8178044345753
3	5.61199555556389	9.34320376113536
4	2.236680648259772	4.965022474536291
5	1.1035630209400438	3.0621354043644615
6	0.6466598276876492	2.1532000424470414
7	0.37836448367015807	1.4698250692551826
8	0.2815270781474111	1.2498773763522897
9	0.205531594887268	1.0265458434722077
>10	1.0056756445046944	9.933181215880717
>50	0.062348407635068215	2.3795737111852064
>100	0.028907526783057448	2.8705383020837436
>500	0.001775166981117952	0.6456320862670536
>1k	8.241721153185768E-4	0.823086690468265
>5k	9.509678249455588E-5	0.33163415086356157
>10k+	1.267957099927412E-4	1.2597418405203962
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24983	0.3297937346978836	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22395	0.2956302561165233	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14191	0.18733150098457613	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10042	0.13256168930217133	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.280290352605909E-5
2	0.0	0.0	0.0	1.3200725881514773E-5	5.280290352605909E-5
3	0.0	0.0	0.0	1.3200725881514773E-5	5.280290352605909E-5
4	0.0	0.0	0.0	1.3200725881514773E-5	6.600362940757387E-5
5	0.0	0.0	0.0	1.3200725881514773E-5	6.600362940757387E-5
6	0.0	0.0	0.0	1.3200725881514773E-5	7.920435528908863E-5
7	0.0	0.0	0.0	1.3200725881514773E-5	7.920435528908863E-5
8	0.0	1.3200725881514773E-5	0.0	1.3200725881514773E-5	7.920435528908863E-5
9	0.0	1.3200725881514773E-5	0.0	2.6401451763029546E-5	7.920435528908863E-5
10-11	0.0	1.3200725881514773E-5	0.0	2.6401451763029546E-5	7.920435528908863E-5
12-13	0.0	1.3200725881514773E-5	0.0	2.6401451763029546E-5	7.920435528908863E-5
14-15	0.0	1.3200725881514773E-5	0.0	3.9602177644544316E-5	1.1880653293363296E-4
16-17	0.0	1.3200725881514773E-5	0.0	4.6202540585301704E-5	1.3200725881514774E-4
18-19	0.0	1.3200725881514773E-5	0.0	7.260399234833124E-5	1.5840871057817726E-4
20-21	0.0	1.3200725881514773E-5	0.0	1.7820979940044946E-4	1.6500907351893465E-4
22-23	0.0	1.3200725881514773E-5	0.0	3.432188729193841E-4	2.0461125116347898E-4
24-25	0.0	1.3200725881514773E-5	0.0	7.85443189950129E-4	2.508137917487807E-4
26-27	0.0	1.3200725881514773E-5	0.0	0.0017490961793007074	2.5741415468953806E-4
28-29	0.0	1.3200725881514773E-5	0.0	0.00467305696205623	2.640145176302955E-4
30-31	0.0	1.3200725881514773E-5	0.0	0.012877308097417661	2.640145176302955E-4
32-33	0.0	1.3200725881514773E-5	0.0	0.03356284555375131	3.300181470378693E-4
34-35	0.0	1.3200725881514773E-5	0.0	0.06684847586399081	3.76220687623171E-4
36-37	0.0	1.3200725881514773E-5	0.0	0.11946656922770868	3.828210505639284E-4
38-39	0.0	1.3200725881514773E-5	0.0	0.19772707261626904	3.828210505639284E-4
40-41	0.0	1.3200725881514773E-5	0.0	0.2904687722968511	3.828210505639284E-4
42-43	0.0	1.3200725881514773E-5	0.0	0.38092674639993107	4.4222431703074493E-4
44-45	0.0	1.3200725881514773E-5	0.0	0.49054557412002975	4.6202540585301707E-4
46-47	0.0	1.3200725881514773E-5	0.0	0.6204803189717796	4.6202540585301707E-4
48-49	0.0	2.6401451763029546E-5	0.0	0.7584411051594906	4.6202540585301707E-4
50-51	0.0	2.6401451763029546E-5	0.0	0.8996954724546393	4.6202540585301707E-4
52-53	0.0	2.6401451763029546E-5	0.0	1.0439926070654773	5.016275834975614E-4
54-55	0.0	2.6401451763029546E-5	0.0	1.2168957146615578	5.478301240828631E-4
56-57	0.0	2.6401451763029546E-5	0.0	1.4159890624065636	5.544304870236205E-4
58-59	0.0	2.6401451763029546E-5	0.0	1.630322648181778	5.544304870236205E-4
60-61	0.0	2.6401451763029546E-5	0.0	1.8510255841948235	5.940326646681648E-4
62-63	0.0	3.300181470378693E-5	0.0	2.080124181868513	6.600362940757386E-4
64-65	0.0	3.9602177644544316E-5	0.0	2.3189319134280555	6.600362940757386E-4
66-67	1.3200725881514773E-5	6.600362940757387E-5	0.0	2.589989018316139	6.600362940757386E-4
68-69	1.3200725881514773E-5	6.600362940757387E-5	0.0	2.896740885987839	6.732370199572534E-4
70-71	1.3200725881514773E-5	6.600362940757387E-5	0.0	3.226046193828106	6.864377458387682E-4
72-73	1.3200725881514773E-5	6.600362940757387E-5	0.0	3.5413521318710273	8.316457305354306E-4
74-75	1.3200725881514773E-5	6.600362940757387E-5	0.0	3.864882122138132	8.316457305354306E-4
76-77	1.3200725881514773E-5	6.600362940757387E-5	0.0	4.216509857444041	8.382460934761881E-4
78-79	1.3200725881514773E-5	6.600362940757387E-5	0.0	4.607878378016251	8.712479081799749E-4
80-81	1.3200725881514773E-5	6.600362940757387E-5	0.0	5.023516433121625	9.174504487652767E-4
82-83	1.3200725881514773E-5	6.600362940757387E-5	0.0	5.4462960809288985	0.0010164558928766375
84-85	1.3200725881514773E-5	6.600362940757387E-5	0.0	5.876243722889834	0.0010296566187581522
86-87	1.9801088822272158E-5	6.600362940757387E-5	0.0	6.323490916118495	0.0010362569816989096
88	2.6401451763029546E-5	6.600362940757387E-5	0.0	6.680319737421721	0.001042857344639667
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	15965	0.0	58.463966	2
GTACCTG	18070	0.0	52.118614	1
ACCTGGG	18140	0.0	50.620678	3
CCTGGGG	15165	0.0	41.12152	4
TATAACG	955	0.0	31.495935	2
CTGGGGG	8720	0.0	31.149157	5
ATAACGC	980	0.0	28.77172	3
TAACGCA	1190	0.0	26.063795	4
GGTATCA	30110	0.0	23.704514	1
TGGGGGG	10805	0.0	23.529215	6
GTATCAA	39830	0.0	22.511812	1
GTATAAC	2130	0.0	22.074478	1
TATCACG	760	0.0	21.643745	2
GTACCCG	1755	0.0	21.432999	1
CATGGGG	18440	0.0	20.948433	4
GTACATG	33705	0.0	20.827423	1
TACCCGG	1605	0.0	20.497501	2
GTACCGT	945	0.0	20.38858	6
TACATGG	33925	0.0	20.323017	2
GAGTACT	24760	0.0	20.104975	12-13
>>END_MODULE