FastQCFastQC Report
Mon 6 Jun 2016
ERR522940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46729016
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1875670.4013930017272352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770890.164970304531985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT625430.13384189386739923No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT485600.10391830206739212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG1525700.027.3814771
TACATGG1551600.026.409432
ACATGGG1582850.025.0143383
CATGGGG1038050.023.0693384
TACCTGG421450.021.2913592
ATGGGGG557450.018.6435475
GAGTACA1181500.018.0593221
GAGTACT859000.017.66463512-13
CCGTATC126450.017.0936894
AGAGTAC1547600.017.02529110-11
AGTACAT1143750.016.994382
GTACTTT912300.016.47066114-15
AGTACTT889600.016.34641612-13
GTACCTG587700.016.0364571
ACCTGGG534250.015.9771883
CGCCGTA169300.015.65376294
TATACTG181100.015.39855
GTATAGG112500.015.34192851
CGTATCA97850.015.12677394
TCGCCGT247250.014.72861894
Produced by FastQC (version 0.11.5)