FastQCFastQC Report
Mon 6 Jun 2016
ERR522940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46729016
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1872940.40080878227780353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724400.15502145390778185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT622460.13320631446636927No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT471490.10089876491300395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG1519800.026.0528431
TACATGG1545500.024.985422
ACATGGG1574150.023.7916663
TACCTGG426850.022.3547022
CATGGGG1029700.022.0748234
ATGGGGG546600.018.2395
GAGTACA1208650.017.636891
GAGTACT878800.017.626812-13
ACCTGGG530250.016.9927273
AGTACAT1163550.016.874372
GTACCTG589600.016.864761
TATACTG189650.016.7294225
AGAGTAC1588250.016.70418710-11
GTACTTT923200.016.61345514-15
AGTACTT906350.016.3157912-13
CTATACT139350.014.1665454
GTGTAGC233250.014.1360821
TACTTTT1050200.013.94656614-15
TATACAG265550.013.8771345
GTATAGG115650.013.8688811
Produced by FastQC (version 0.11.5)