FastQCFastQC Report
Mon 6 Jun 2016
ERR522939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591136
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT422930.7564294626351424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194230.3473891531166475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151850.27159060341225827No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT112610.20140808594174778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93010.16635259811244085No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC89150.15944881326442426No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC81900.1464818598581755No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA66180.1183659277828334No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG63890.1142701590517562No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA58030.10378928360891239No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG178200.027.625421
TACATGG182000.026.3506222
TACCTGG41550.025.0082532
ACATGGG187650.024.756543
CATGGGG122400.023.2385354
ATGGGGG74700.021.5258265
GTACCTG53000.020.226881
GAGTACT167300.020.20449312-13
AGAGTAC239650.019.13198910-11
ACCTGGG54000.018.2855133
AGTACTT177500.018.0509112-13
GAGTACA139100.017.982681
GTACTTT189150.017.74547214-15
CGTATAC3551.8171704E-917.2185523
GAGTACG5800.017.0239831
AGTACAT136950.016.7883852
GTGTAGC28600.015.78247451
TATAACG4351.6407284E-915.1321592
TGGGGGG79350.015.0976136
GTATAGC18800.015.0059421
Produced by FastQC (version 0.11.5)