FastQCFastQC Report
Mon 6 Jun 2016
ERR522939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591136
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT434000.7762286590775113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177820.31803912478609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148330.2652949239653623No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT113530.20305354761536834No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC91440.16354458199550145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89510.16009268957149317No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84000.1502378049827441No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG66470.11888460591908336No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61470.10994187943201524No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC57540.10291289641317972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG179350.028.6331521
TACATGG182300.027.6903532
ACATGGG186300.026.3094883
CATGGGG121450.026.2732144
TACCTGG41150.025.3567732
ATGGGGG74500.023.1499865
GTACCTG52800.020.6634081
GAGTACT173500.020.12001412-13
AGAGTAC248450.019.40546810-11
GTACTTT188500.018.35371414-15
AGTACTT182350.018.07355312-13
ACCTGGG56200.017.8108333
TGGGGGG76600.017.1779046
GTATAGG16250.017.0744421
GAGTACA138800.016.6017741
GTGTAGC30300.016.2965091
CATGGGA69600.015.9346944
AGTACAT134150.015.8715152
TAACGCA5155.456968E-1215.512534
ACTTTTT230500.015.179790516-17
Produced by FastQC (version 0.11.5)