FastQCFastQC Report
Mon 6 Jun 2016
ERR522938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6367738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT609830.9576870153891381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259280.40717755661429533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207860.3264267468290938No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT168650.26485072093104334No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC135470.21274430574876038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124500.19551683816136908No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC119750.18805736040019236No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98330.15441904173821222No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG91170.14317486052347003No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC81580.1281145675277469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58750.038.0099372
GTACCTG70200.032.4168661
ACCTGGG71100.031.078023
TATCACG6500.028.9306412
GTACATG238400.028.3408781
TACATGG245100.027.179282
CATGGGG163550.026.1578624
ACATGGG249450.025.9145873
ATGGGGG105000.025.8357985
TGGGGGG110950.021.1320466
CCTGGGG70450.020.5532784
GAGTACT215850.020.54969612-13
TATAACG6350.019.9894752
AGAGTAC323300.019.27032510-11
ATAACGC6650.018.3813253
AGTACTT224850.018.36882812-13
GTACTTT241400.018.24745814-15
GTATAAC18000.016.9786681
GTACACG11500.016.7628841
GAGTACA182800.016.692881
Produced by FastQC (version 0.11.5)