FastQCFastQC Report
Mon 6 Jun 2016
ERR522938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6367738
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT620990.9752128620869766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240910.3783290078831761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205920.3233801390697921No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT171410.2691850701143797No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC140100.22001533354544422No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC125360.19686739624023478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118570.18620426908267895No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA100450.15774832444425319No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG98530.15473312501236702No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA91480.14366168959840997No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC82530.12960646307998225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59300.039.0775952
GTACCTG72450.032.3936921
ACCTGGG72150.031.590693
TATAACG5250.029.5454252
TATCACG5700.026.3882562
GTACATG233450.026.3317681
TACATGG242050.025.1866552
TAACGCA6300.024.6165494
ACATGGG246650.024.1025583
CATGGGG163900.023.5406254
ATGGGGG104600.023.3175325
ATAACGC7350.022.378683
TGGGGGG104250.020.8263346
GAGTACT208150.020.31554812-13
CCTGGGG68750.019.7550834
AGAGTAC319250.019.10646210-11
AGTACTT218700.018.29298612-13
GTACTTT230650.018.11711314-15
GGGTTAG18850.016.717131
GTATAGG18200.016.5389121
Produced by FastQC (version 0.11.5)