FastQCFastQC Report
Mon 6 Jun 2016
ERR522937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8677992
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT903071.040643964640668No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT263980.3041947952936578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220260.2538144768974205No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC181050.20863121330372278No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC179320.20663766456571983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158220.1823232840039493No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG147560.17003933628885576No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA131790.15186692958463202No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC111030.1279443447285962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99650.1148307119895939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG300200.028.4730591
TACATGG305150.027.3624782
ACATGGG306400.026.2547073
CATGGGG201650.024.3404224
ATGGGGG114650.020.7913325
GAGTACT214400.019.08595812-13
GAGTACA232050.018.2960411
AGAGTAC350300.018.19764110-11
GTACTTT231100.017.3812814-15
AGTACTT225850.017.16146912-13
AGTACAT225300.017.0902542
CATGGGA113650.016.0504824
TACCTGG55800.015.7555372
GTACAGG56500.015.0619451
GTATAGG23450.014.6362891
GTATATG34500.014.581951
CGTATAC5501.4551915E-1114.5322273
GTATTAG23600.014.3440381
ACTTTTT281450.014.2543816-17
ATGGGGA86000.014.050225
Produced by FastQC (version 0.11.5)