FastQCFastQC Report
Mon 6 Jun 2016
ERR522937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8677992
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT921401.0617663625410116No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT259220.29870965541337213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197000.2270110412639237No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC192600.22194074389559243No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC192390.22169875243028572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150800.17377291889644517No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG149710.17251686795747218No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA137530.15848136296968238No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA125910.14509116855604384No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC116370.13409784198925281No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93510.10775534248014977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG293800.026.9121761
TACATGG298500.026.013462
ACATGGG305950.024.34623
CATGGGG204450.023.1240164
ATGGGGG120650.019.6315945
GAGTACT206100.018.39527912-13
GAGTACA237100.017.7353131
AGAGTAC348800.017.28441410-11
AGTACAT229600.016.8279952
GTACTTT224000.016.73420514-15
TACCTGG58200.016.3947752
AGTACTT219950.016.29655812-13
GTGTAGC45150.015.73084451
GTATAGG22750.015.299711
TATACTG38150.015.27494055
CATGGGA109950.014.9597794
GTACCTG75950.013.8105171
ACTTTTT276500.013.75053316-17
TGGGGGG105000.013.7404646
GTGTAGG32000.013.6699161
Produced by FastQC (version 0.11.5)