FastQCFastQC Report
Mon 6 Jun 2016
ERR522936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7894537
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208830.26452469600180484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164430.2082832723439006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93480.11841099737704694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.048.1441651
GTATCAA203250.039.849731
TCAACGC239250.033.4131744
CAACGCA242500.033.003085
ATCAACG243700.032.766143
TATCAAC252800.031.6852512
AACGCAG254500.031.4953426
TACCTGG74100.029.7522132
ACGCAGA292300.027.3420187
CGCAGAG302300.026.4203368
GTACATG240900.025.9048961
TACATGG241100.025.4240672
GTACCTG95100.024.100361
ACATGGG249600.023.763053
ACCTGGG92050.023.5887873
TATCACG7900.023.2060992
GCAGAGT341350.023.1214399
CATGGGG172700.021.5525264
AGAGTAC305250.019.84522410-11
GAGTACT200450.019.84037212-13
Produced by FastQC (version 0.11.5)