FastQCFastQC Report
Mon 6 Jun 2016
ERR522936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7894537
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191670.24278814577726343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156400.19811168153369857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88650.1122928425061533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112200.049.1034851
GTATCAA201300.039.5929531
TCAACGC241650.032.417924
TACCTGG72150.032.3345572
ATCAACG245100.031.963433
CAACGCA246450.031.80565
TATCAAC254400.031.0239072
AACGCAG257250.030.6891426
ACGCAGA296500.026.4837727
GTACCTG91800.026.0357041
GTACATG235950.025.6230851
CGCAGAG307050.025.4731318
TACATGG240900.024.737822
ACCTGGG93500.024.427883
ACATGGG243100.023.5076773
GCAGAGT347050.022.266299
TATAACG6800.022.134142
CATGGGG176250.020.850384
AGAGTAC307200.019.8300910-11
GAGTACT206750.019.64305912-13
Produced by FastQC (version 0.11.5)