FastQCFastQC Report
Mon 6 Jun 2016
ERR522935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8710684
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263610.3026283584618613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219090.2515187096673464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122330.1404367326377584No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129300.054.58171
GTATCAA240700.042.905481
TCAACGC297500.034.2007064
CAACGCA302600.033.515585
ATCAACG304300.033.409023
TATCAAC315100.032.5518382
AACGCAG313850.032.3664676
ACGCAGA362550.027.9669597
CGCAGAG377550.026.7916098
GTACATG315900.025.8555851
TACATGG329600.024.3310572
GCAGAGT418800.023.7131969
ACATGGG336150.023.0566753
GTGGTAT70950.021.8171391
CATGGGG233150.021.2657764
GAGTACT234050.020.77547512-13
CAGAGTA402900.020.67786210-11
AGAGTAC381750.020.08227310-11
TGGTATC72450.019.9839552
GTACTTT250200.019.32128514-15
Produced by FastQC (version 0.11.5)