FastQCFastQC Report
Mon 6 Jun 2016
ERR522935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8710684
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242420.278301910619189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211110.24235754620417865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119320.13698120606831793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128100.055.1224061
GTATCAA234600.042.4912571
TCAACGC293350.033.2564934
ATCAACG299000.032.644723
CAACGCA300000.032.4566465
TATCAAC308350.031.7678052
AACGCAG313450.031.078946
ACGCAGA360400.026.9904887
CGCAGAG375000.025.9196558
GTACATG309800.025.465271
TACATGG322250.023.9035762
GCAGAGT416200.023.2635049
ACATGGG329300.022.8764933
GTGGTAT69900.021.6708161
GAGTACT237950.020.83982812-13
CATGGGG230450.020.3715444
CAGAGTA404550.020.22200610-11
AGAGTAC381350.019.88076210-11
TGGTATC69300.019.5434462
GTACTTT253400.019.41903114-15
Produced by FastQC (version 0.11.5)