FastQCFastQC Report
Mon 6 Jun 2016
ERR522934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066491
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251180.49576718877029485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206390.4073628079078794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120420.2376792932228637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116650.23023824576023127No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62260.1228858395287784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84600.052.474832
GTACCTG98550.045.77311
ACCTGGG97350.044.830133
TATAACG5850.035.3709072
CCTGGGG87300.033.1261984
ATAACGC6800.029.7383983
GGTATCA195200.029.4469131
GTATCAA280150.028.0061741
TAACGCA8500.027.1073994
TATCACG6100.024.6700232
CTGGGGG54200.023.7692055
TCAACGC329850.023.3796544
TATCAAC332300.023.2801072
CAACGCA332600.023.2262295
ATCAACG332150.023.206133
AACGCAG343000.022.7017616
TACACGG10350.022.2641012
GTACACG11950.022.0428891
CATGGGG143750.021.229884
GAGTACT206300.020.88531912-13
Produced by FastQC (version 0.11.5)