##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522934_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5066491
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.419383158876627	33.0	31.0	34.0	28.0	34.0
2	31.745657102716653	34.0	31.0	34.0	28.0	35.0
3	31.732604676491086	34.0	31.0	34.0	27.0	36.0
4	34.042374495484154	35.0	34.0	37.0	32.0	37.0
5	34.13576793090129	35.0	34.0	37.0	31.0	37.0
6	34.368624162166675	35.0	35.0	37.0	30.0	37.0
7	34.26235021437914	35.0	34.0	37.0	31.0	37.0
8	34.37055093949639	36.0	34.0	37.0	30.0	37.0
9	35.54566306344963	37.0	35.0	39.0	30.0	39.0
10-11	35.51506210116627	37.0	34.5	39.0	30.0	39.0
12-13	35.5458957688862	37.5	35.0	39.0	30.0	39.0
14-15	36.31794737225429	38.0	35.0	41.0	30.0	41.0
16-17	36.47091882725144	38.0	36.0	41.0	30.5	41.0
18-19	36.3482269089198	38.0	35.5	41.0	29.5	41.0
20-21	36.44957131079479	38.0	35.5	41.0	30.0	41.0
22-23	36.28637334991812	38.0	35.5	40.5	30.0	41.0
24-25	36.26264183633209	38.0	35.0	40.5	30.0	41.0
26-27	36.138371902762685	38.0	36.0	40.0	29.5	41.0
28-29	35.900521879936235	38.0	35.0	40.0	29.0	41.0
30-31	35.69542805859125	37.0	35.5	40.0	28.5	41.0
32-33	35.629493667313334	37.5	34.5	40.0	27.5	41.0
34-35	35.452991330686274	37.5	34.5	40.0	27.0	41.0
36-37	35.25069086276873	37.5	34.5	40.0	27.0	41.0
38-39	35.06521061618386	36.5	34.5	40.0	26.0	41.0
40-41	34.99905753311316	37.0	34.0	40.0	25.5	41.0
42-43	34.77286340783	36.0	35.0	40.0	25.0	41.0
44-45	34.68086551421881	37.0	33.5	40.0	25.0	41.0
46-47	34.42905217832224	36.5	33.5	40.0	23.0	41.0
48-49	34.25563580395189	37.0	33.0	40.0	22.5	41.0
50-51	33.20345126439581	36.5	31.5	38.5	21.0	40.0
52-53	33.410215275226975	36.5	32.5	38.5	22.0	39.5
54-55	34.240807592473764	37.0	33.0	39.5	23.0	41.0
56-57	34.36328703633343	37.0	34.0	40.0	23.0	41.0
58-59	34.2946113000102	37.0	33.0	40.0	22.0	41.0
60-61	34.042033727090406	36.5	33.5	39.5	22.0	41.0
62-63	33.86618045901986	36.5	33.0	39.0	22.0	41.0
64-65	33.46769292593237	36.0	33.0	39.0	21.0	41.0
66-67	33.26755618434929	36.0	33.0	38.5	21.0	40.5
68-69	32.92996237435338	35.0	32.5	37.5	20.0	40.0
70-71	32.71883794918416	35.0	32.0	37.5	19.5	40.0
72-73	32.391362088672416	35.0	32.0	37.5	19.5	39.0
74-75	32.00969221103916	35.0	31.5	36.5	18.5	39.0
76-77	31.882973541253698	35.0	31.0	37.0	18.0	38.5
78-79	31.61126616034648	35.0	31.0	36.5	18.0	38.0
80-81	31.349856340413908	35.0	30.0	36.0	18.0	37.5
82-83	31.034510374142577	34.5	30.0	35.5	17.0	37.0
84-85	30.801800694010904	35.0	30.0	35.0	17.5	37.0
86-87	30.56183984142082	34.0	29.5	35.0	17.0	37.0
88-89	30.405760318137347	34.0	29.0	35.0	17.0	36.5
90-91	30.196824685961154	34.0	29.0	35.0	17.0	36.0
92-93	29.935137060344132	34.0	29.0	35.0	15.0	36.0
94-95	29.83370472778892	34.0	29.0	35.0	15.0	36.0
96-97	29.648577980302342	34.0	28.5	35.0	12.5	36.0
98-99	29.554658737181217	34.0	27.5	35.0	10.5	36.0
100	28.351883581753132	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07305930764011848
1101	2	0.013247758418629019
1101	3	0.1276600389126088
1101	4	0.1014789392709119
1101	5	0.024458631200985792
1101	6	0.07555011082961727
1101	7	0.06099472017326235
1101	8	0.12556122300517814
1101	9	0.1361957223228103
1101	10-11	0.08320264929661647
1101	12-13	0.10576012608422758
1101	14-15	-0.03638141406276674
1101	16-17	0.058413863624984685
1101	18-19	0.06224297393508493
1101	20-21	0.042070442694168264
1101	22-23	0.035317886718083
1101	24-25	-0.08035832812801402
1101	26-27	0.018815874687334144
1101	28-29	-0.06055160756568512
1101	30-31	-0.10064560612219253
1101	32-33	-0.06767600694472264
1101	34-35	-0.14689581709285449
1101	36-37	-0.10011552436522209
1101	38-39	-0.19882120666558478
1101	40-41	-0.12904486642887036
1101	42-43	-0.22534132950735852
1101	44-45	-0.19248784815311382
1101	46-47	-0.2016831842663649
1101	48-49	-0.2592692726820829
1101	50-51	-0.30104824633671967
1101	52-53	-0.29528065734259457
1101	54-55	-0.1916697447961866
1101	56-57	-0.21152409593963029
1101	58-59	-0.24465038896266833
1101	60-61	-0.2030692443737081
1101	62-63	-0.39891442001972877
1101	64-65	-0.3044151885092248
1101	66-67	-0.25804665818672845
1101	68-69	-0.24304661526175408
1101	70-71	-0.454019624793645
1101	72-73	-0.2944127425331189
1101	74-75	-0.34545110683834324
1101	76-77	-0.37717558183755884
1101	78-79	-0.5127694045754474
1101	80-81	-0.3175895287556365
1101	82-83	-0.6232683015048757
1101	84-85	-0.57186671448871
1101	86-87	-0.5008756518713504
1101	88-89	-0.47440103311064874
1101	90-91	-0.5689122087161813
1101	92-93	-0.5054921150613296
1101	94-95	-0.349417266039751
1101	96-97	-0.5170339186889095
1101	98-99	-0.3985767622964964
1101	100	-0.46031548925402177
1102	1	0.11550964669358521
1102	2	0.019592248007612056
1102	3	0.04732936496022333
1102	4	0.09314519517706543
1102	5	0.06254669356147247
1102	6	0.13070351227878518
1102	7	0.14566654599202877
1102	8	0.230826233954879
1102	9	0.1811599157417234
1102	10-11	0.15775392277158318
1102	12-13	0.09406753456091366
1102	14-15	0.01834597004995686
1102	16-17	0.14777892175347063
1102	18-19	0.12842705567408075
1102	20-21	0.060199064721736306
1102	22-23	0.1458957915577841
1102	24-25	0.05825926321926289
1102	26-27	0.12291902017401668
1102	28-29	0.12917926214343112
1102	30-31	0.011407515775097465
1102	32-33	0.06481010348178273
1102	34-35	0.03539383244627459
1102	36-37	-0.48014059249278773
1102	38-39	-0.1485934106187372
1102	40-41	-0.8059846536314481
1102	42-43	-0.8362396275504977
1102	44-45	-0.2590715411952971
1102	46-47	-0.09567352317900912
1102	48-49	-0.2750424765437032
1102	50-51	-0.1893472048827718
1102	52-53	-0.09845008549353196
1102	54-55	0.05448553676254164
1102	56-57	0.07328961829779956
1102	58-59	0.006411133573990924
1102	60-61	-0.10940352362983674
1102	62-63	-0.32067862470521646
1102	64-65	-0.05169360668221401
1102	66-67	-0.08503432832959845
1102	68-69	-0.07533309607689631
1102	70-71	-0.07865610173589488
1102	72-73	-0.035279775832890437
1102	74-75	0.06642491859594557
1102	76-77	-0.1787624083956878
1102	78-79	-0.19074782908588261
1102	80-81	-0.3389379264375414
1102	82-83	-0.2195658235627178
1102	84-85	-0.3254104041509258
1102	86-87	-0.28850953045992966
1102	88-89	-0.2871628929403798
1102	90-91	-0.2847533641033628
1102	92-93	-0.38179041169614436
1102	94-95	-0.47842658208828226
1102	96-97	-0.5633078513570453
1102	98-99	-0.39071832680116003
1102	100	-0.6020208260893831
1103	1	0.045175159723783054
1103	2	0.009215200381426314
1103	3	0.011744177394955813
1103	4	0.10692929180834199
1103	5	0.06560116733884058
1103	6	0.11586640274932591
1103	7	0.09729043915554314
1103	8	-0.008275187745077517
1103	9	0.05857486564671177
1103	10-11	0.0463754125409892
1103	12-13	0.03966491182874421
1103	14-15	-0.07502599858258918
1103	16-17	-0.09553585597076619
1103	18-19	-0.04360129458901696
1103	20-21	-0.09467702038123349
1103	22-23	-0.12859140799218238
1103	24-25	-0.1750219110855724
1103	26-27	-0.18048469201099238
1103	28-29	-0.28872776815780554
1103	30-31	-0.20808196674049384
1103	32-33	-0.32297804387235374
1103	34-35	-0.22245031415483396
1103	36-37	-0.3113035550857788
1103	38-39	-0.25561341525494186
1103	40-41	-0.15286193494024047
1103	42-43	-0.25665021968626434
1103	44-45	-0.12924626475364676
1103	46-47	-0.09523296193848552
1103	48-49	-0.17517873073213508
1103	50-51	-0.20322650397887543
1103	52-53	-0.11591751815046791
1103	54-55	-0.1375199724831262
1103	56-57	0.006370133157773239
1103	58-59	0.035602250092097165
1103	60-61	-0.009498604067182725
1103	62-63	-0.017356355335977014
1103	64-65	-0.17034080932241125
1103	66-67	0.017421785858829253
1103	68-69	-0.15690208403282213
1103	70-71	-0.08389692904678725
1103	72-73	-0.10609551389226368
1103	74-75	-0.09208697493752638
1103	76-77	-0.039185559261909475
1103	78-79	-0.13626984387216012
1103	80-81	-0.11956565340957681
1103	82-83	-0.07784661390181213
1103	84-85	-0.05492286528044232
1103	86-87	-0.7860940387950599
1103	88-89	-0.3326856819699131
1103	90-91	-0.6144957165682605
1103	92-93	-0.22244046296241038
1103	94-95	-0.5211619481439662
1103	96-97	-0.3183964704399287
1103	98-99	-0.24241493571250317
1103	100	-0.2627611515938675
1104	1	0.03057761551033522
1104	2	-0.03494132122455085
1104	3	0.030390480482800797
1104	4	0.023200167675142325
1104	5	-0.11758844407018643
1104	6	0.06295424242480152
1104	7	-0.031794771032046754
1104	8	0.002170943039565998
1104	9	-0.042006166621192165
1104	10-11	-0.09524488827721456
1104	12-13	0.005218566497326549
1104	14-15	-0.19764399264851562
1104	16-17	-0.08646819650792281
1104	18-19	-0.027758933868824442
1104	20-21	-0.09629991523466686
1104	22-23	-0.0959543751890024
1104	24-25	-0.14254002522445575
1104	26-27	-0.16029528333118037
1104	28-29	-0.11592301520616388
1104	30-31	-0.13189759866146034
1104	32-33	-0.10261319036169425
1104	34-35	-0.09939573655231726
1104	36-37	-0.06772291845609857
1104	38-39	-0.08844900368733022
1104	40-41	-0.07681072234319686
1104	42-43	-0.3401641520772358
1104	44-45	-0.21293918724017402
1104	46-47	-0.06309528299832579
1104	48-49	-0.10244523260779914
1104	50-51	-0.21038510913525243
1104	52-53	-0.1237001231843422
1104	54-55	-0.14376700353198402
1104	56-57	-0.05449706854981429
1104	58-59	-0.05736726113075008
1104	60-61	-0.09232420583841616
1104	62-63	-0.010683413994627244
1104	64-65	-0.1878267664123925
1104	66-67	-0.10906995084484805
1104	68-69	-0.2620873623960236
1104	70-71	-0.13832044570303736
1104	72-73	-0.10432541981458598
1104	74-75	-0.022892978385588236
1104	76-77	-0.10027554673530759
1104	78-79	-0.12375006612197481
1104	80-81	-0.22605768638420187
1104	82-83	-0.12555414254605424
1104	84-85	-0.1418926401511449
1104	86-87	-0.13221469530484953
1104	88-89	-0.04992993273230795
1104	90-91	-0.2560933185378964
1104	92-93	-0.18412414079671535
1104	94-95	-0.25261006061507985
1104	96-97	-0.38707979491287503
1104	98-99	-0.32516813419040247
1104	100	-0.19077971147072503
1105	1	0.1004719513885739
1105	2	0.04204759982873085
1105	3	0.02888662212274795
1105	4	0.10395720169923806
1105	5	0.14445230447626756
1105	6	0.16603404200328953
1105	7	0.03386930972699531
1105	8	-0.052209956233042476
1105	9	0.00300277198127219
1105	10-11	0.07526457106033746
1105	12-13	0.11897960649141481
1105	14-15	0.1875926548015272
1105	16-17	0.14020432672014493
1105	18-19	0.11056780485336759
1105	20-21	0.04045371145976162
1105	22-23	0.05857884935033297
1105	24-25	0.07656650405561294
1105	26-27	0.06893521780459366
1105	28-29	-0.04419923852361052
1105	30-31	-0.09294362016331092
1105	32-33	0.002651379970345147
1105	34-35	-0.011911686627357199
1105	36-37	-0.08739602392341084
1105	38-39	-0.12139310461972741
1105	40-41	-0.0643922563385857
1105	42-43	-0.030844736448628396
1105	44-45	-0.14059058994471485
1105	46-47	-0.18663736823487653
1105	48-49	-0.09750618321587012
1105	50-51	-0.290279486016253
1105	52-53	-0.06838296680800937
1105	54-55	0.018290228125422914
1105	56-57	0.05123741395431125
1105	58-59	-0.006494291792620288
1105	60-61	0.06690713585400943
1105	62-63	0.08897230630092423
1105	64-65	0.045511084058730944
1105	66-67	0.09839831301613344
1105	68-69	0.015160306596868622
1105	70-71	0.03351807721340805
1105	72-73	0.1107872557797478
1105	74-75	0.09588144901081463
1105	76-77	0.06397750592044105
1105	78-79	0.025584951058181815
1105	80-81	0.04253331514930281
1105	82-83	0.04985100576094581
1105	84-85	-0.0329919857470351
1105	86-87	0.008416050207397774
1105	88-89	0.012245591898050634
1105	90-91	-0.04353181439251941
1105	92-93	0.019561187594419494
1105	94-95	-0.06874013778227805
1105	96-97	-0.01933709955520868
1105	98-99	-0.05757600098062454
1105	100	0.0654643533792445
1106	1	-0.06734348691579228
1106	2	-0.0514253384935337
1106	3	0.016812011118062742
1106	4	0.08451305102485662
1106	5	0.04517110583985584
1106	6	0.03681004841008928
1106	7	0.11698507706519479
1106	8	-1.1429318157600434
1106	9	-0.07435935240926739
1106	10-11	-0.081258798319773
1106	12-13	-0.03659458670063742
1106	14-15	-0.0026692937701895403
1106	16-17	-0.03412518373846751
1106	18-19	0.03640214649419704
1106	20-21	-0.018297719177709837
1106	22-23	-0.005700104246379567
1106	24-25	0.05940177951021752
1106	26-27	-0.027871364099461005
1106	28-29	-0.11142032941857138
1106	30-31	-0.0014831374644330708
1106	32-33	-0.02346234893318666
1106	34-35	-0.04626515099463546
1106	36-37	0.10739734548738511
1106	38-39	0.03432057764425167
1106	40-41	-0.04367492780285431
1106	42-43	-0.019620867636675143
1106	44-45	-0.08741389458177196
1106	46-47	-0.016327777773064156
1106	48-49	-0.10084504306300346
1106	50-51	0.005048192612711944
1106	52-53	-0.05459472058800685
1106	54-55	0.05684380888702378
1106	56-57	-0.02621866458014921
1106	58-59	0.03351233593275538
1106	60-61	0.05299666537756309
1106	62-63	0.04104121009764583
1106	64-65	-0.05895890758501565
1106	66-67	0.039723019396646464
1106	68-69	0.06561621841539278
1106	70-71	-0.043969298913076216
1106	72-73	0.028190537526604942
1106	74-75	0.11994845858727032
1106	76-77	0.005340014882701638
1106	78-79	-0.059289536392608255
1106	80-81	-0.0362823676035795
1106	82-83	0.031865772905835144
1106	84-85	0.07238044025615054
1106	86-87	0.04499166406466415
1106	88-89	-0.004544891179257604
1106	90-91	-0.12706457611863087
1106	92-93	-0.04449199376490043
1106	94-95	-0.09547431587785127
1106	96-97	-0.13596197132201837
1106	98-99	-0.11096304480032515
1106	100	-0.16007861381658373
1107	1	-0.1171158171169644
1107	2	-0.1259848331329252
1107	3	-0.112571762203455
1107	4	0.010356657420111048
1107	5	-0.040365664727204376
1107	6	-0.01808239856308802
1107	7	-0.034462896351783456
1107	8	-0.02963567981557702
1107	9	0.02937593286630147
1107	10-11	0.017864063346223702
1107	12-13	0.03492085072068818
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2316	82-83	-0.21057764217925978
2316	84-85	-0.10750704691847801
2316	86-87	0.062125947861993325
2316	88-89	-0.05865306987953289
2316	90-91	0.10609492373375318
2316	92-93	0.23374989139595215
2316	94-95	0.18855519425079237
2316	96-97	0.2800274595771839
2316	98-99	0.3148474958726055
2316	100	-0.09251363477216046
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	9066.0
1	805.0
2	507.0
3	385.0
4	317.0
5	322.0
6	435.0
7	1724.0
8	3646.0
9	4608.0
10	6215.0
11	8720.0
12	11744.0
13	14142.0
14	16303.0
15	18190.0
16	22058.0
17	27385.0
18	33897.0
19	40406.0
20	47228.0
21	55279.0
22	62244.0
23	66575.0
24	67598.0
25	69842.0
26	73756.0
27	81528.0
28	93090.0
29	110396.0
30	133460.0
31	165331.0
32	211961.0
33	282454.0
34	408129.0
35	663818.0
36	735877.0
37	711488.0
38	658983.0
39	146411.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.51212253704756	17.143319104390994	12.29523329113934	23.0493250674221
2	16.823783933685434	19.99091803138158	39.56062470828768	23.624673326645308
3	19.295949631492196	26.543728437715004	29.555979032920693	24.604342897872108
4	13.299412748472179	16.378577960753677	36.52391286399804	33.798096426776105
5	13.583761583341056	35.858820445443364	33.53763808736379	17.019779883851786
6	29.91894054181984	34.550923715298715	19.99105165954909	15.53908408333236
7	27.5476928368182	32.205911076009926	22.224645268354887	18.02175081881699
8	26.90372526879742	32.93621723366725	21.128583058569678	19.031474438965663
9	27.908064775962615	15.956105668431078	20.748953761254512	35.386875794351795
10-11	25.304056650989747	25.20271185380944	28.267521376738003	21.225710118462807
12-13	25.905286911976088	23.45001598880467	28.09280472128822	22.55189237793102
14-15	23.752146576837436	24.482138492523173	26.773353633307256	24.992361297332135
16-17	22.749394820308794	26.939362137489425	27.292483782490045	23.018759259711736
18-19	22.434819618862132	25.853566213654744	29.542602304128913	22.169011863354214
20-21	23.48589555940791	25.259366139646833	29.3677701150608	21.88696818588446
22-23	23.11444431298234	25.09830309682949	29.166955201803784	22.620297388384387
24-25	22.61292836932692	25.21410613982144	29.269586219252247	22.90337927159939
26-27	22.48599289861031	25.17724785337882	29.26573070988967	23.0710285381212
28-29	22.6298427966876	25.595583494280454	29.259835187378858	22.514738521653086
30-31	23.437811456628026	25.31210829112321	29.187720619490975	22.062359632757797
32-33	22.91353018771434	25.449528317427216	29.153001536773687	22.483939958084754
34-35	23.02065266991211	25.73910989377646	28.88722724841537	22.353010187896057
36-37	23.24706133746985	25.634703620970406	28.652350435383063	22.46588460617668
38-39	23.207563857043166	25.897852869557852	28.37155710535183	22.523026168047153
40-41	23.155136146996032	25.628180281564163	28.666497866298034	22.55018570514177
42-43	23.00816894597526	25.7939029896609	28.264471950831993	22.933456113531847
44-45	22.851936650403854	25.973191977891386	28.38024793255994	22.79462343914482
46-47	22.783324557385058	25.654627542852857	28.239464979416006	23.322582920346075
48-49	22.950021908023103	25.80043922890486	28.13153400976972	23.118004853302317
50-51	22.77746715954065	26.23582532254835	28.04870810511796	22.93799941279304
52-53	22.89285985305887	26.373666275101996	27.539713077056188	23.193760794782943
54-55	22.746739965152283	26.15734984772305	27.887050566792432	23.208859620332237
56-57	22.90108375717792	26.266843790233242	27.5356664673268	23.29640598526204
58-59	22.855422670212107	26.29477459649991	27.803500092758643	23.046302640529344
60-61	23.227598956508906	26.26503411546363	27.79944600519213	22.707920922835335
62-63	23.052696959163242	26.274425055761057	27.821060359089252	22.851817625986452
64-65	22.951329480821148	25.970261962121583	27.75464120066665	23.323767356390622
66-67	22.88003294616771	26.057414555030324	27.670209415364834	23.39234308343714
68-69	22.70764348246597	26.08657232364911	27.668375232429625	23.5374089614553
70-71	22.684689993623095	26.036618262692667	27.751088295147287	23.52760344853695
72-73	22.693226252448834	26.37802827898255	27.273040418229932	23.65570505033868
74-75	22.812516576267456	26.324577369505686	27.236598656797835	23.626307397429024
76-77	22.676213628021795	26.116919764060547	27.433488023663216	23.77337858425444
78-79	22.633930267832827	26.20033748266275	27.47427911866597	23.691453130838457
80-81	22.80256065203404	26.29196898730251	27.131905280689335	23.77356507997412
82-83	22.9409038828066	26.372908036478616	27.079851613293293	23.60633646742149
84-85	22.935648982936453	26.543171512324115	26.793127472888536	23.7280520318509
86-87	22.707454694052686	26.437676434989736	27.041336812546145	23.81353205841143
88-89	22.64138451189594	26.149297341666454	27.20470748770859	24.00461065872901
90-91	23.205035626999667	26.133413398380245	27.085693317553872	23.57585765706622
92-93	22.86387562918853	26.230846815485325	27.07996563988006	23.825311915446086
94-95	22.76077721339405	26.04257050750201	27.130811366702346	24.065840912401594
96-97	22.8065444494078	25.99413923995767	27.127241945561746	24.072074365072783
98-99	22.8925643058342	25.988369124650674	26.854233512768154	24.264833056746973
100	22.89999972289113	26.00066981172036	27.039768685351984	24.059561780036525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5155.0
1	5388.0
2	7842.0
3	7593.5
4	3756.5
5	2548.5
6	3282.5
7	5228.5
8	8068.0
9	9530.0
10	8362.0
11	6957.5
12	6980.0
13	7462.5
14	8058.0
15	8593.0
16	8932.5
17	9533.0
18	10194.5
19	10601.5
20	11083.0
21	12012.0
22	13290.0
23	14966.0
24	17235.0
25	20068.0
26	24338.0
27	29511.5
28	34479.0
29	40392.0
30	47715.5
31	54601.5
32	61680.0
33	69693.5
34	77995.0
35	85676.0
36	94636.5
37	104291.5
38	111219.0
39	117267.0
40	122472.0
41	127549.5
42	134108.0
43	142314.5
44	150663.0
45	158547.5
46	167253.5
47	176879.0
48	188327.5
49	201247.0
50	215960.5
51	216974.5
52	204367.0
53	196285.0
54	190609.0
55	181490.0
56	168514.5
57	154665.5
58	141184.0
59	125624.0
60	105839.0
61	86561.0
62	71024.5
63	55983.5
64	42325.0
65	32664.0
66	25260.0
67	20003.0
68	16000.0
69	12470.5
70	10077.5
71	8238.0
72	6817.5
73	5834.5
74	4899.0
75	3837.0
76	2819.5
77	1966.0
78	1397.5
79	992.5
80	711.0
81	541.5
82	396.0
83	286.0
84	199.5
85	145.0
86	114.0
87	85.0
88	71.5
89	63.5
90	46.0
91	33.5
92	27.0
93	24.0
94	20.0
95	14.5
96	12.5
97	8.0
98	3.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14445895591248462
2	0.07326569809361154
3	0.3101949653122842
4	0.3093659892023888
5	0.30993837746874514
6	0.25737734459609224
7	0.2598247978729263
8	0.2577128825453356
9	0.26900274766105375
10-11	0.24588023545290025
12-13	0.2556996548498754
14-15	0.22975467636279231
16-17	0.23686018587618135
18-19	0.24968957805313383
20-21	0.25121923635115506
22-23	0.24129126055883648
24-25	0.24238669327548398
26-27	0.24215971172158404
28-29	0.2528870573341589
30-31	0.23913000141518062
32-33	0.25109094242938557
34-35	0.2351627586035384
36-37	0.24318606309574023
38-39	0.24375845136209656
40-41	0.2602294171646609
42-43	0.2461861671125045
44-45	0.233100187092013
46-47	0.23599173471343382
48-49	0.24487362160517012
50-51	0.23793588106640276
52-53	0.2533212829155327
54-55	0.26374269686850327
56-57	0.2594497848708307
58-59	0.2576438012028443
60-61	0.2390510513094763
62-63	0.26561776187898095
64-65	0.2598445353993523
66-67	0.2644729853462683
68-69	0.26877576610715387
70-71	0.28950016885453855
72-73	0.29817481171880106
74-75	0.2780425347641987
76-77	0.2748154491935345
78-79	0.27212127683637455
80-81	0.25683456261937504
82-83	0.2686770784750235
84-85	0.2802926127767719
86-87	0.2726837963395178
88-89	0.28194069623334966
90-91	0.27499308693136926
92-93	0.2557588674291536
94-95	0.27591088191018204
96-97	0.27443056742822597
98-99	0.2608708867735085
100	0.2827400660536059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5066491.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.37861810406376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00199851463375	45.128936449460596
2	14.929901597414997	17.73033850568313
3	4.317175247097235	7.690437008571115
4	1.6752742697885823	3.97901884341362
5	0.8245227133781311	2.447950965790324
6	0.4955921389991438	1.7656545814203746
7	0.33352721551020204	1.3863069609964442
8	0.2327253454382451	1.1055127527931097
9	0.172784657131122	0.9233742753027442
>10	0.93906603909927	9.97832489346818
>50	0.052622515947272665	2.1633942528315817
>100	0.0223133163628019	2.315504912419168
>500	0.0014898046734349905	0.5820595942035035
>1k	8.052996207014452E-4	0.9935297401297856
>5k	4.0264981035069356E-5	0.14885612229690337
>10k+	1.6105992414027742E-4	1.660800141219418
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25118	0.49576718877029485	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20639	0.4073628079078794	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12042	0.2376792932228637	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11665	0.23023824576023127	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	6226	0.1228858395287784	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9737526426080693E-5	0.0
3	0.0	0.0	0.0	1.9737526426080693E-5	0.0
4	0.0	0.0	0.0	3.9475052852161386E-5	1.9737526426080693E-5
5	0.0	0.0	0.0	3.9475052852161386E-5	1.9737526426080693E-5
6	0.0	0.0	0.0	5.921257927824208E-5	3.9475052852161386E-5
7	0.0	0.0	0.0	5.921257927824208E-5	3.9475052852161386E-5
8	0.0	0.0	0.0	7.895010570432277E-5	5.921257927824208E-5
9	0.0	0.0	0.0	7.895010570432277E-5	7.895010570432277E-5
10-11	0.0	0.0	0.0	7.895010570432277E-5	7.895010570432277E-5
12-13	0.0	9.868763213040346E-6	0.0	7.895010570432277E-5	8.881886891736311E-5
14-15	0.0	1.9737526426080693E-5	0.0	9.868763213040347E-5	1.4803144819560522E-4
16-17	0.0	1.9737526426080693E-5	0.0	9.868763213040347E-5	1.5790021140864554E-4
18-19	0.0	1.9737526426080693E-5	0.0	9.868763213040347E-5	1.7763773783472625E-4
20-21	0.0	1.9737526426080693E-5	0.0	1.3816268498256486E-4	1.7763773783472625E-4
22-23	0.0	1.9737526426080693E-5	0.0	4.144880549476946E-4	1.7763773783472625E-4
24-25	0.0	1.9737526426080693E-5	0.0	0.0012928079809082856	1.9737526426080694E-4
26-27	0.0	1.9737526426080693E-5	0.0	0.002585615961816571	2.3685031711296833E-4
28-29	0.0	1.9737526426080693E-5	0.0	0.005605457505006918	2.3685031711296833E-4
30-31	0.0	1.9737526426080693E-5	0.0	0.014615638318512755	2.3685031711296833E-4
32-33	0.0	3.9475052852161386E-5	0.0	0.03341563223935461	2.763253699651297E-4
34-35	0.0	3.9475052852161386E-5	0.0	0.06312060951060605	3.355379492433718E-4
36-37	0.0	3.9475052852161386E-5	0.0	0.10907943979373495	3.355379492433718E-4
38-39	9.868763213040346E-6	3.9475052852161386E-5	0.0	0.18223658149200306	3.6514423888249286E-4
40-41	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.271499544753953	4.5396310779985595E-4
42-43	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.3555320635129915	4.5396310779985595E-4
44-45	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.45715071831766796	4.5396310779985595E-4
46-47	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.5781812303623948	4.83569397438977E-4
48-49	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.7042744179354112	4.934381606520173E-4
50-51	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.8433450291335759	4.934381606520173E-4
52-53	1.9737526426080693E-5	3.9475052852161386E-5	0.0	0.9853565317692264	5.822570295693805E-4
54-55	1.9737526426080693E-5	3.9475052852161386E-5	0.0	1.153776844762973	6.710758984867436E-4
56-57	1.9737526426080693E-5	3.9475052852161386E-5	0.0	1.3446683315928125	6.710758984867436E-4
58-59	1.9737526426080693E-5	3.9475052852161386E-5	0.0	1.5538762429460549	6.710758984867436E-4
60-61	1.9737526426080693E-5	3.9475052852161386E-5	0.0	1.7706238893940598	6.710758984867436E-4
62-63	1.9737526426080693E-5	3.9475052852161386E-5	0.0	1.9990364139598786	6.710758984867436E-4
64-65	1.9737526426080693E-5	3.9475052852161386E-5	0.0	2.228011458028841	6.710758984867436E-4
66-67	1.9737526426080693E-5	3.9475052852161386E-5	0.0	2.4869085921597414	6.710758984867436E-4
68-69	1.9737526426080693E-5	3.9475052852161386E-5	0.0	2.786129492779125	6.710758984867436E-4
70-71	1.9737526426080693E-5	5.921257927824208E-5	0.0	3.1089169999512483	6.908134249128243E-4
72-73	1.9737526426080693E-5	5.921257927824208E-5	0.0	3.4156973731918203	7.895010570432277E-4
74-75	1.9737526426080693E-5	5.921257927824208E-5	0.0	3.725181787552766	7.895010570432277E-4
76-77	1.9737526426080693E-5	5.921257927824208E-5	0.0	4.075137999850389	7.993698202562682E-4
78-79	1.9737526426080693E-5	5.921257927824208E-5	0.0	4.462615249883992	8.092385834693085E-4
80-81	1.9737526426080693E-5	5.921257927824208E-5	0.0	4.857997379251241	8.585823995345102E-4
82-83	1.9737526426080693E-5	5.921257927824208E-5	0.0	5.269149792232929	9.67138794877954E-4
84-85	1.9737526426080693E-5	5.921257927824208E-5	0.0	5.692421046440229	9.67138794877954E-4
86-87	2.9606289639121038E-5	5.921257927824208E-5	0.0	6.137433186005857	9.67138794877954E-4
88	3.9475052852161386E-5	5.921257927824208E-5	0.0	6.4827708171197775	9.67138794877954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8460	0.0	52.47483	2
GTACCTG	9855	0.0	45.7731	1
ACCTGGG	9735	0.0	44.83013	3
TATAACG	585	0.0	35.370907	2
CCTGGGG	8730	0.0	33.126198	4
ATAACGC	680	0.0	29.738398	3
GGTATCA	19520	0.0	29.446913	1
GTATCAA	28015	0.0	28.006174	1
TAACGCA	850	0.0	27.107399	4
TATCACG	610	0.0	24.670023	2
CTGGGGG	5420	0.0	23.769205	5
TCAACGC	32985	0.0	23.379654	4
TATCAAC	33230	0.0	23.280107	2
CAACGCA	33260	0.0	23.226229	5
ATCAACG	33215	0.0	23.20613	3
AACGCAG	34300	0.0	22.701761	6
TACACGG	1035	0.0	22.264101	2
GTACACG	1195	0.0	22.042889	1
CATGGGG	14375	0.0	21.22988	4
GAGTACT	20630	0.0	20.885319	12-13
>>END_MODULE