FastQCFastQC Report
Mon 6 Jun 2016
ERR522934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066491
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219140.43252815410113227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197740.3902898475493196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117370.23165934766290908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102680.20266492134299657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61590.12156342525823098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG87300.053.9953162
GTACCTG101750.046.7618061
ACCTGGG102200.045.474923
CCTGGGG88600.035.376814
TATAACG6450.033.5171852
ATAACGC7350.031.328193
TATCACG4700.029.9980342
GGTATCA197450.029.3596651
TAACGCA8050.027.436494
GTATCAA276350.026.7107071
CTGGGGG55400.026.3801445
TCAACGC326550.022.4204484
CAACGCA330200.022.201085
ATCAACG330400.022.187643
TATCAAC331250.022.076142
AACGCAG338200.021.884346
GTACACG12100.021.3708841
GAGTACT208500.020.46908812-13
CATGGGG138100.020.314554
ACGCAGA373700.019.7676937
Produced by FastQC (version 0.11.5)