##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522934_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5066491
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2235598563187	33.0	31.0	34.0	30.0	34.0
2	32.35479802490521	34.0	31.0	34.0	30.0	34.0
3	32.54231399996566	34.0	31.0	34.0	30.0	35.0
4	35.64543270677871	37.0	35.0	37.0	33.0	37.0
5	35.57905481328201	36.0	35.0	37.0	33.0	37.0
6	35.703073389452385	37.0	35.0	37.0	33.0	37.0
7	35.54822440225394	37.0	35.0	37.0	33.0	37.0
8	35.53620799879049	36.0	35.0	37.0	33.0	37.0
9	36.82644418000545	38.0	37.0	39.0	33.0	39.0
10-11	36.633782533118094	37.5	35.0	39.0	33.0	39.0
12-13	36.63726699603335	37.5	35.0	39.0	33.0	39.0
14-15	37.80770201703704	38.0	37.0	41.0	33.0	41.0
16-17	37.621341772836466	38.0	36.5	41.0	33.0	41.0
18-19	37.53727639109593	38.0	36.0	41.0	32.0	41.0
20-21	37.55500562420815	38.0	36.5	41.0	33.0	41.0
22-23	37.40360172355976	38.0	36.0	41.0	33.0	41.0
24-25	37.326590336388634	38.0	36.5	41.0	32.0	41.0
26-27	37.01049513361417	38.0	36.0	40.0	32.5	41.0
28-29	36.96847877554702	38.0	36.0	40.0	32.0	41.0
30-31	36.70843617406998	38.0	35.5	40.0	31.5	41.0
32-33	36.63364713368681	37.5	36.0	40.0	30.5	41.0
34-35	36.38481051283817	37.5	35.0	40.0	30.5	41.0
36-37	36.14697104958836	37.0	35.5	40.0	30.5	41.0
38-39	36.07993510696062	37.0	35.0	40.0	30.0	41.0
40-41	36.253621392004845	37.5	35.0	40.0	30.0	41.0
42-43	36.27890190666479	37.5	35.0	40.0	30.5	41.0
44-45	36.078869083158345	37.0	35.0	40.0	30.0	41.0
46-47	35.94091837920959	37.0	35.5	40.0	29.0	41.0
48-49	35.69756178388553	37.5	35.0	40.0	28.5	41.0
50-51	35.522210934550166	38.0	34.5	40.0	27.5	41.0
52-53	35.52140830803805	37.5	35.0	40.0	27.5	41.0
54-55	35.32166217210294	37.5	34.0	40.0	27.0	41.0
56-57	35.07559521965005	37.0	34.5	40.0	26.5	41.0
58-59	34.893218699095684	37.0	34.0	40.0	26.5	41.0
60-61	34.71073362214598	37.0	34.0	39.0	26.0	41.0
62-63	34.457713928634234	36.5	34.0	39.0	26.0	41.0
64-65	34.27548247889911	36.5	33.5	39.0	25.0	40.5
66-67	33.784209722271285	35.5	33.5	38.0	25.0	40.0
68-69	33.64227263011027	36.5	33.0	37.0	23.5	40.0
70-71	33.22946897566777	35.0	32.5	37.0	24.0	39.5
72-73	32.99862439309574	35.0	32.5	37.0	23.5	39.0
74-75	32.8083942120888	35.0	32.0	37.0	22.5	39.0
76-77	31.216976996505075	34.0	29.5	36.0	21.0	37.0
78-79	32.322084259105566	35.0	31.5	36.5	23.0	37.0
80-81	32.43141545104886	35.0	32.0	36.0	23.0	37.5
82-83	32.32463336064349	35.0	32.0	37.0	23.0	37.0
84-85	31.993430759079608	35.0	32.0	36.0	22.0	36.5
86-87	31.8701725711148	35.0	32.0	36.0	21.5	36.5
88-89	31.82628055591138	35.0	31.5	36.0	22.0	37.0
90-91	31.590545902479644	34.5	31.0	35.0	21.5	36.5
92-93	31.37432060966851	35.0	31.0	35.0	21.0	36.5
94-95	31.508707801908656	34.0	31.0	35.0	20.5	36.5
96-97	31.237924828051604	34.5	31.0	35.0	20.0	37.0
98-99	31.082098142481648	34.0	31.0	35.0	20.0	36.0
100	29.893409462288595	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06148465173610873
1101	2	-0.12517695428380193
1101	3	-0.15224582194011305
1101	4	-0.0905695314446433
1101	5	-0.19884624574525844
1101	6	-0.08522076000879508
1101	7	-0.10185458406707681
1101	8	-0.14120785162716487
1101	9	-0.11625113075431415
1101	10-11	-0.14607640837565583
1101	12-13	-0.13794064354520685
1101	14-15	-0.31078229167559357
1101	16-17	-0.2987732397924461
1101	18-19	-0.37003600315284046
1101	20-21	-0.27774194758676884
1101	22-23	-0.7804101425545156
1101	24-25	-0.265907830914621
1101	26-27	-0.1890282201221396
1101	28-29	-0.3165291664360552
1101	30-31	-0.4016675713245803
1101	32-33	-0.2923317687801372
1101	34-35	-0.4072775221894389
1101	36-37	-0.3416253808444196
1101	38-39	-0.22401982911596008
1101	40-41	-0.23042602014614744
1101	42-43	-0.23542207121261782
1101	44-45	-0.3993356216935098
1101	46-47	-0.3400926162073574
1101	48-49	-0.4009363030641495
1101	50-51	-0.4431870981306858
1101	52-53	-0.45564165507552445
1101	54-55	-0.41062017200533063
1101	56-57	-0.5560506375990357
1101	58-59	-0.5128695083201222
1101	60-61	-0.6560275878956929
1101	62-63	-0.5191095946711499
1101	64-65	-0.5499356780757552
1101	66-67	-0.4728989040888436
1101	68-69	-0.5066119097674715
1101	70-71	-0.5887328701314516
1101	72-73	-0.6707569363247856
1101	74-75	-0.5890240653843719
1101	76-77	-0.6802036294438523
1101	78-79	-0.5946167840874921
1101	80-81	-0.45128034107039383
1101	82-83	-0.3643823105677484
1101	84-85	-0.38447702231405145
1101	86-87	-0.40717263674666526
1101	88-89	-0.5356811997373931
1101	90-91	-0.45269883178531956
1101	92-93	-0.437191634349837
1101	94-95	-0.5089940576868344
1101	96-97	-0.5577547044559843
1101	98-99	-0.725635107639345
1101	100	-0.6086617942195787
1102	1	-0.030839569947822554
1102	2	-0.11653337939981157
1102	3	-0.15391116243711167
1102	4	-0.09035476237420426
1102	5	-0.09747172369446133
1102	6	-0.15892528001752737
1102	7	-0.0934398614712535
1102	8	-0.2881028541973478
1102	9	-0.21520686015936974
1102	10-11	-0.2555346974055297
1102	12-13	-0.15558339456095638
1102	14-15	-0.34019333150997255
1102	16-17	-0.2973314703769674
1102	18-19	-0.3078850028922986
1102	20-21	-0.2837818875922622
1102	22-23	-0.24355084094128188
1102	24-25	-0.18918246070203537
1102	26-27	-0.21265281787023582
1102	28-29	-0.28360084297271015
1102	30-31	-0.36165714974345065
1102	32-33	-0.2739866172799239
1102	34-35	-0.34045793525022106
1102	36-37	-0.41820639807524174
1102	38-39	-0.3592908958492913
1102	40-41	-0.2803492666094982
1102	42-43	-0.3322565159465469
1102	44-45	-0.2051368511584215
1102	46-47	-0.20887927658352368
1102	48-49	-0.3602586890429009
1102	50-51	-0.2656593365877242
1102	52-53	-0.38454781155415674
1102	54-55	-0.4060888966995506
1102	56-57	-0.40580382920825997
1102	58-59	-0.23376653887366672
1102	60-61	-0.4377447546339468
1102	62-63	-0.4973668668927118
1102	64-65	-0.5257371892050173
1102	66-67	-0.48994487875314263
1102	68-69	-0.48348022464004714
1102	70-71	-0.4514338200444925
1102	72-73	-0.4689937174645351
1102	74-75	-0.49216497501940637
1102	76-77	-0.46822127571567407
1102	78-79	-0.5286316277989798
1102	80-81	-0.6169922829348664
1102	82-83	-0.5645805318573487
1102	84-85	-0.4589402756781773
1102	86-87	-0.49991238592455645
1102	88-89	-0.5410979573581756
1102	90-91	-0.5231994197596599
1102	92-93	-0.4771686082888422
1102	94-95	-0.6361144621568506
1102	96-97	-0.7032695516882832
1102	98-99	-0.7081279075942781
1102	100	-0.6768210472457099
1103	1	-0.05614280715563069
1103	2	-0.08556718399621843
1103	3	-0.15700763049509447
1103	4	-0.054012794110690265
1103	5	-0.1503883043282528
1103	6	-0.17236921456014187
1103	7	-0.1493073608477573
1103	8	-0.16288478795486583
1103	9	-0.17632293348710704
1103	10-11	-0.210065326952396
1103	12-13	-0.23786880999359283
1103	14-15	-0.20559833794356308
1103	16-17	-0.32296929428047605
1103	18-19	-0.3400910815349576
1103	20-21	-0.3065094615628894
1103	22-23	-0.36627430805641836
1103	24-25	-0.2983092485464667
1103	26-27	-0.4020523253252861
1103	28-29	-0.3710358807029692
1103	30-31	-0.45164582264886377
1103	32-33	-0.28042853151051617
1103	34-35	-0.3033673705445423
1103	36-37	-0.3838230338023294
1103	38-39	-0.3217645458057561
1103	40-41	-0.39134248562721297
1103	42-43	-0.3675605143532934
1103	44-45	-0.2928205514150264
1103	46-47	-0.4226734636241076
1103	48-49	-0.3877926935750011
1103	50-51	-0.4468793007360503
1103	52-53	-0.4459663194435066
1103	54-55	-1.2146964071704502
1103	56-57	-0.49013541385991033
1103	58-59	-0.4442945555178781
1103	60-61	-0.35697071251907175
1103	62-63	-0.38825402805030507
1103	64-65	-0.3314085715039923
1103	66-67	-0.2852829750009178
1103	68-69	-0.3544522504319474
1103	70-71	-0.45103817753599174
1103	72-73	-0.5217152435013546
1103	74-75	-0.608463190965189
1103	76-77	-0.4957132859671596
1103	78-79	-0.3716407014670047
1103	80-81	-1.1311868547238575
1103	82-83	-0.27507463328094417
1103	84-85	-0.12254791007008237
1103	86-87	-0.19358401194183728
1103	88-89	-0.33911916413325827
1103	90-91	-0.3235709386999446
1103	92-93	-0.3356258966339851
1103	94-95	-0.36785750125768857
1103	96-97	-0.4431624687974214
1103	98-99	-0.3609704049310274
1103	100	-0.32002219635734264
1104	1	-0.09086667763929057
1104	2	-0.18374837997878046
1104	3	-0.10185462985295146
1104	4	-0.05248308728909734
1104	5	-0.07253125372884028
1104	6	-0.25942926468651706
1104	7	-0.1659042762344427
1104	8	-0.08401442413747162
1104	9	-0.2154854587408721
1104	10-11	-0.17310912561864455
1104	12-13	-0.16042206552861416
1104	14-15	-0.27228882418410905
1104	16-17	-0.24908824556786868
1104	18-19	-0.2691390165098113
1104	20-21	-0.2732061312111327
1104	22-23	-0.16603746606503478
1104	24-25	-0.19259211806041776
1104	26-27	-0.2186211615497058
1104	28-29	-0.3790364654219829
1104	30-31	-0.3240927743370605
1104	32-33	-0.25726039308740667
1104	34-35	-0.22795301565123083
1104	36-37	-0.3494873115486925
1104	38-39	-0.26476226518523305
1104	40-41	-0.26168254309046546
1104	42-43	-0.2798457969774617
1104	44-45	-0.3588048142679412
1104	46-47	-0.37170662166934676
1104	48-49	-0.4064717029781022
1104	50-51	-0.34535933507019223
1104	52-53	-0.4232786226438634
1104	54-55	-0.36431985178958115
1104	56-57	-0.35025526794147765
1104	58-59	-0.4223538609722226
1104	60-61	-0.4847703065280058
1104	62-63	-0.47109581653482024
1104	64-65	-0.6254132750549459
1104	66-67	-0.44183911817901134
1104	68-69	-0.3641781140867195
1104	70-71	-0.3225515636565959
1104	72-73	-0.39625604433836514
1104	74-75	-0.549794407070884
1104	76-77	-0.4025107734563669
1104	78-79	-0.36525747732671476
1104	80-81	-0.3853469232589859
1104	82-83	-0.37699776877962776
1104	84-85	-1.0828579226632584
1104	86-87	-0.4091439082736379
1104	88-89	-0.41158035929692005
1104	90-91	-0.4141013327702652
1104	92-93	-0.2768472178559378
1104	94-95	-0.21056696398530406
1104	96-97	-0.2770780314109551
1104	98-99	-0.23229878815383742
1104	100	-1.4477582502347133
1105	1	-0.016671110858091254
1105	2	-0.19623716572294114
1105	3	-0.21458139563696932
1105	4	-0.07401093226584976
1105	5	-0.11503279133247446
1105	6	-0.106157326157863
1105	7	-0.1384444794441393
1105	8	-0.23608223450789012
1105	9	-0.1591648827340535
1105	10-11	-0.17098721210167156
1105	12-13	-0.1584201671006582
1105	14-15	-0.25183756826945825
1105	16-17	-0.28229792140002274
1105	18-19	-0.32842819260027767
1105	20-21	-0.24570014480764968
1105	22-23	-0.3063743037678961
1105	24-25	-0.20964123117064304
1105	26-27	-0.2740078586130892
1105	28-29	-0.22296852794299582
1105	30-31	-0.27453199594059186
1105	32-33	-0.20363427268689094
1105	34-35	-0.2577076551139257
1105	36-37	-0.3387054708526307
1105	38-39	-0.2920423058599724
1105	40-41	-0.2049722358157524
1105	42-43	-0.21686542910067175
1105	44-45	-0.21522078320424498
1105	46-47	-0.35822302687505925
1105	48-49	-0.38235637860524463
1105	50-51	-0.23037141038407327
1105	52-53	-0.412153752827642
1105	54-55	-1.2829471202674796
1105	56-57	-0.26494866977675713
1105	58-59	-0.2821957543158291
1105	60-61	-0.2956769186336601
1105	62-63	-0.19075260290221507
1105	64-65	-0.17972957447353366
1105	66-67	-0.2255345332499914
1105	68-69	-0.09179050266170918
1105	70-71	-0.15219875537732008
1105	72-73	-0.2697873868508509
1105	74-75	-0.22015266871771644
1105	76-77	-0.3676964749726501
1105	78-79	-0.2984542167869577
1105	80-81	-0.15584408176746933
1105	82-83	-0.22004531968455865
1105	84-85	-0.24672218711468474
1105	86-87	-0.28588896539048747
1105	88-89	-0.37946291508117724
1105	90-91	-0.39734024357285236
1105	92-93	-0.25370478655607087
1105	94-95	-0.18743808409880103
1105	96-97	-0.24235764975723129
1105	98-99	-0.09097585448811074
1105	100	-0.010920055340378099
1106	1	-0.09154984291694745
1106	2	-0.14220704843879162
1106	3	-0.14110827321121633
1106	4	-0.10094338981210171
1106	5	-0.11193752084865594
1106	6	-0.0957850177763504
1106	7	-0.09138717928079387
1106	8	-0.11099725540874772
1106	9	-0.08827870062093268
1106	10-11	-0.23326878610462387
1106	12-13	-0.15623890903973603
1106	14-15	-0.21159858149319888
1106	16-17	-0.1749497377343019
1106	18-19	-0.23143040900165346
1106	20-21	-0.2792593458394421
1106	22-23	-0.20967707933245094
1106	24-25	-0.10634174901282023
1106	26-27	-0.09987149316358312
1106	28-29	-0.12110167771833602
1106	30-31	-0.19334714683897403
1106	32-33	-0.03131204249821451
1106	34-35	-0.10929750119318982
1106	36-37	-0.18911989801975437
1106	38-39	-0.24558407842875596
1106	40-41	-0.14228254530393514
1106	42-43	-0.20597292610131746
1106	44-45	-0.22507569943204686
1106	46-47	-0.24891256601289768
1106	48-49	-0.21182351153102985
1106	50-51	-0.11601381391640331
1106	52-53	-0.30257198480007474
1106	54-55	-0.28761048193570815
1106	56-57	-0.24533802818634598
1106	58-59	-0.33168338409600295
1106	60-61	-0.1288075472140875
1106	62-63	-0.1440094037359998
1106	64-65	-0.09275616413039245
1106	66-67	-0.11554155986623726
1106	68-69	-0.12951269255218278
1106	70-71	-0.19103286893439275
1106	72-73	-0.16332449546474237
1106	74-75	-0.1929824517517602
1106	76-77	-0.26826598886786357
1106	78-79	-0.11810683397138177
1106	80-81	-0.12518704712226025
1106	82-83	-0.0899096047340251
1106	84-85	0.09288962046444738
1106	86-87	0.018036959550521203
1106	88-89	-0.16532818610707523
1106	90-91	-0.14917071290376782
1106	92-93	0.039432203822478584
1106	94-95	-0.06761285967154151
1106	96-97	0.021453496890398327
1106	98-99	-0.22649039815182803
1106	100	-0.1923997768531791
1107	1	-0.08482600662284057
1107	2	-0.13384979044108292
1107	3	-0.1590238042519232
1107	4	-0.04191956370556227
1107	5	-0.13499133100999927
1107	6	-0.11785968699853555
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2316	100	0.09458879272083465
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	5.0
12	28.0
13	202.0
14	788.0
15	2199.0
16	4823.0
17	9360.0
18	15171.0
19	21438.0
20	28128.0
21	35650.0
22	44095.0
23	50377.0
24	54319.0
25	58269.0
26	64917.0
27	75018.0
28	89665.0
29	108111.0
30	132011.0
31	161697.0
32	202642.0
33	261407.0
34	369308.0
35	654645.0
36	1015591.0
37	743834.0
38	715298.0
39	147403.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98612127514289	17.899514904167606	12.913963516907268	23.20040030378223
2	16.983729874543325	20.46274633969552	38.941312743141886	23.612211042619265
3	19.211975309933443	26.42363324044196	29.95149897631319	24.41289247331141
4	13.401089830485727	16.804737651969536	36.41919945934807	33.374973058196666
5	13.666525411100084	36.004675447407436	33.47158878168949	16.857210359802995
6	30.1148586328718	34.807279755270365	19.662492112387888	15.41536949946995
7	27.61727988858561	32.22658443486823	22.008388053980557	18.1477476225656
8	27.033380913958542	33.33407678060226	20.82166186912907	18.810880436310125
9	27.653221924207504	16.011199861995216	20.621668922336976	35.713909291460304
10-11	25.39537970119553	25.376964587222655	28.081785606821924	21.145870104759894
12-13	25.824271800969196	23.55010547773678	28.059900459333438	22.56572226196059
14-15	23.628690900340732	24.618806913954185	26.632725152298587	25.1197770334065
16-17	22.737184712646627	27.054334331410207	27.253212827228744	22.955268128714415
18-19	22.38113462422096	25.93069400342012	29.50548782509699	22.18268354726193
20-21	23.40617890421663	25.338193016271653	29.33385190829434	21.92177617121737
22-23	23.022894224311784	25.2323395906702	29.14235355014184	22.602412634876178
24-25	22.564668804273925	25.3362615967036	29.236310873719084	22.862758725303387
26-27	22.525916627949776	25.27336000529846	29.219985674009997	22.980737692741766
28-29	22.639669312182367	25.70185784885127	29.222739525110025	22.43573331385634
30-31	23.37811525480472	25.361688746728657	29.219468459864846	22.040727538601782
32-33	22.92716945289054	25.50444956254456	29.1248265929955	22.4435543915694
34-35	23.015859856241555	25.774112030837752	28.888009825507233	22.322018287413457
36-37	23.239987284406592	25.67133625549855	28.63804486993805	22.450631590156807
38-39	23.185458203150674	25.927322597117136	28.431774089030625	22.455445110701564
40-41	23.17145999259602	25.653866162040593	28.717093020627814	22.457580824735572
42-43	23.048097351010764	25.83239721277877	28.32364248702311	22.795862949187356
44-45	22.86589623569333	25.982350812272614	28.39910894430073	22.752644007733323
46-47	22.80521180766121	25.729204173615322	28.16016769948959	23.305416319233878
48-49	22.94648456034265	25.894787275404347	28.106372119021817	23.052356045231186
50-51	22.81184420026893	26.33526389719242	27.904983369750408	22.947908532788247
52-53	22.9249744492966	26.452585247559295	27.397815692449974	23.224624610694136
54-55	22.77612435820608	26.229583590919393	27.753960114230043	23.24033193664449
56-57	22.95865934887786	26.372433289129344	27.35397113999421	23.314936221998586
58-59	22.876071995414886	26.332898503077846	27.7225557280686	23.068473773438665
60-61	23.247929530513307	26.363724825876076	27.66985902905102	22.718486614559595
62-63	23.10902546471353	26.391402001566572	27.635529706365258	22.864042827354645
64-65	22.944417732891843	26.051560138889545	27.609616711589446	23.394405416629166
66-67	22.87525756479866	26.166994658087077	27.512938810035088	23.444808967079176
68-69	22.700086519472702	26.190264481945537	27.50222812067409	23.60742087790767
70-71	22.668720819874377	26.13659947772454	27.578980569067284	23.615699133333802
72-73	22.685761380683978	26.526922640788253	27.07388353982451	23.71343243870326
74-75	22.75323336694145	26.498686598887222	26.896625028916933	23.851455005254394
76-77	22.570927777870985	26.271992373049468	27.28547474510214	23.8716051039774
78-79	22.511516464520152	26.379807014283603	27.273569732430765	23.835106788765483
80-81	22.631380169642114	26.525210404561182	26.905513176332445	23.93789624946425
82-83	22.757681787879967	26.598489375445023	26.866537154485464	23.777291682189542
84-85	22.72678915672995	26.816779463607766	26.579148951372346	23.87728242828994
86-87	22.50643692113353	26.708599173981913	26.77134496864191	24.013618936242654
88-89	22.31013445232193	26.492505644944814	26.949686962151237	24.24767294058202
90-91	22.90562408620997	26.454339241295834	26.806300445033944	23.833736227460257
92-93	22.556006538685182	26.640365604242174	26.775252631915524	24.028375225157124
94-95	22.271612102264093	26.50785849769762	26.810296674818268	24.410232725220023
96-97	22.271817941285637	26.478919637269748	26.853573521840268	24.395688899604345
98-99	22.34486176627589	26.436613159034582	26.616629890593522	24.601895184095998
100	22.15110560915728	26.59093102651287	26.830722613694746	24.427240750635104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2479.0
1	1892.5
2	4216.0
3	5812.0
4	3282.0
5	2104.0
6	2754.0
7	4738.5
8	7174.5
9	8364.0
10	7357.0
11	6025.0
12	6115.5
13	6664.5
14	7181.0
15	7829.5
16	8499.0
17	9076.0
18	9644.0
19	10285.0
20	11126.5
21	12064.5
22	13280.5
23	15108.5
24	17370.5
25	20387.5
26	24901.0
27	30213.5
28	35253.0
29	41050.5
30	48350.0
31	55162.5
32	62466.0
33	70681.0
34	78619.5
35	85646.0
36	94674.5
37	104953.5
38	111815.5
39	117476.0
40	122864.5
41	128849.0
42	135620.5
43	143312.0
44	151452.0
45	159734.5
46	168976.0
47	178645.5
48	190500.5
49	203881.5
50	219461.0
51	220875.0
52	207537.5
53	198685.0
54	192426.0
55	183304.5
56	169299.0
57	154681.0
58	141920.0
59	125810.5
60	104721.0
61	85033.5
62	69269.0
63	54433.0
64	41341.5
65	31591.5
66	24009.5
67	18774.5
68	14682.0
69	11297.0
70	9129.5
71	7392.0
72	6039.0
73	5261.0
74	4449.5
75	3340.5
76	2276.5
77	1480.5
78	1042.0
79	720.0
80	522.0
81	348.5
82	222.0
83	159.5
84	103.5
85	60.5
86	36.5
87	27.0
88	17.0
89	7.5
90	5.0
91	5.0
92	3.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04612659925775058
2	0.01024377621513588
3	0.0
4	2.960628963912104E-4
5	4.7370063422593666E-4
6	3.9475052852161386E-5
7	0.0
8	5.921257927824208E-5
9	0.0
10-11	9.868763213040346E-6
12-13	3.750130020955332E-4
14-15	0.01223726638417003
16-17	0.0015987396405125363
18-19	5.921257927824208E-5
20-21	8.388448731084295E-4
22-23	7.598947674041067E-4
24-25	0.004628449946915923
26-27	0.017220991806755404
28-29	0.013539943128291357
30-31	0.01052010158510101
32-33	0.00740157240978026
34-35	0.009730600528057782
36-37	0.005339000898254827
38-39	0.004184355602329107
40-41	0.005871914111759006
42-43	0.007302884777649857
44-45	0.007214065908732493
46-47	0.01505973266309957
48-49	0.018069705443076872
50-51	0.017220991806755404
52-53	0.011516846669618086
54-55	0.004401468393015994
56-57	0.004075799206985664
58-59	0.010451020242609728
60-61	0.0025264033825383287
62-63	1.875065010477666E-4
64-65	3.750130020955332E-4
66-67	9.375325052388329E-4
68-69	2.960628963912104E-5
70-71	1.480314481956052E-4
72-73	0.002615222251455692
74-75	0.005033069238650577
76-77	0.00485543150081585
78-79	0.003108660412107709
80-81	2.960628963912104E-5
82-83	8.585823995345103E-4
84-85	2.3685031711296833E-4
86-87	3.158004228172911E-4
88-89	2.1711279068688762E-4
90-91	4.638318710128963E-4
92-93	5.723882663563402E-4
94-95	1.9737526426080693E-5
96-97	0.0016086084037255767
98-99	3.4540671245641215E-4
100	1.5790021140864554E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5066491.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.32860078807928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91856518080064	43.69895080046617
2	15.486295443310619	18.065878891982322
3	4.5633867790790115	7.985278970354957
4	1.7915956299437625	4.180050650906285
5	0.9015015320778409	2.629166148720511
6	0.5135088828585002	1.797135277763161
7	0.3419843477742067	1.396322794796536
8	0.2487102088079572	1.1605534785183338
9	0.17722026845146463	0.930330926105555
>10	0.9743272029804829	10.179922707241152
>50	0.055647185734260134	2.2390324220337248
>100	0.024711738607046892	2.5472574188389085
>500	0.0016012644002956766	0.6370085831481518
>1k	7.390449187424598E-4	0.8802542521282573
>5k	4.1058051041247576E-5	0.14749934538192194
>10k+	1.642322041649903E-4	1.525357331614021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21914	0.43252815410113227	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19774	0.3902898475493196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11737	0.23165934766290908	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10268	0.20266492134299657	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	6159	0.12156342525823098	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9475052852161386E-5
2	0.0	0.0	0.0	0.0	3.9475052852161386E-5
3	0.0	0.0	0.0	0.0	3.9475052852161386E-5
4	0.0	0.0	0.0	1.9737526426080693E-5	3.9475052852161386E-5
5	0.0	0.0	0.0	1.9737526426080693E-5	3.9475052852161386E-5
6	0.0	0.0	0.0	3.9475052852161386E-5	9.868763213040347E-5
7	0.0	0.0	0.0	3.9475052852161386E-5	9.868763213040347E-5
8	0.0	0.0	0.0	3.9475052852161386E-5	1.1842515855648416E-4
9	0.0	0.0	0.0	3.9475052852161386E-5	2.1711279068688762E-4
10-11	0.0	0.0	0.0	3.9475052852161386E-5	2.1711279068688762E-4
12-13	0.0	0.0	0.0	7.895010570432277E-5	2.1711279068688762E-4
14-15	0.0	0.0	0.0	1.3816268498256486E-4	2.368503171129683E-4
16-17	0.0	0.0	0.0	1.3816268498256486E-4	2.56587843539049E-4
18-19	0.0	0.0	0.0	1.5790021140864554E-4	2.56587843539049E-4
20-21	0.0	0.0	0.0	1.9737526426080694E-4	2.56587843539049E-4
22-23	0.0	0.0	0.0	4.737006342259366E-4	2.763253699651297E-4
24-25	0.0	0.0	0.0	0.0013717580866126084	3.158004228172911E-4
26-27	0.0	0.0	0.0	0.0026843035939469744	3.2566918603033144E-4
28-29	0.0	0.0	0.0	0.005585719978580836	3.355379492433718E-4
30-31	0.0	0.0	0.0	0.014536688212808431	3.4540671245641215E-4
32-33	0.0	0.0	0.0	0.03327746955437205	3.552754756694525E-4
34-35	0.0	0.0	0.0	0.06289362795670612	3.7501300209553316E-4
36-37	0.0	0.0	0.0	0.10890180205590022	3.848817653085735E-4
38-39	0.0	9.868763213040346E-6	0.0	0.18201946870131613	3.9475052852161387E-4
40-41	0.0	1.9737526426080693E-5	0.0	0.2715686260964443	4.046192917346542E-4
42-43	0.0	1.9737526426080693E-5	0.0	0.3556208823819089	4.144880549476946E-4
44-45	0.0	1.9737526426080693E-5	0.0	0.45716058708088103	4.144880549476946E-4
46-47	1.9737526426080693E-5	1.9737526426080693E-5	0.0	0.5775693670431863	4.144880549476946E-4
48-49	1.9737526426080693E-5	1.9737526426080693E-5	0.0	0.703218460271616	4.144880549476946E-4
50-51	1.9737526426080693E-5	1.9737526426080693E-5	0.0	0.8413515389645417	4.144880549476946E-4
52-53	1.9737526426080693E-5	1.9737526426080693E-5	0.0	0.9819912835135797	4.83569397438977E-4
54-55	1.9737526426080693E-5	1.9737526426080693E-5	0.0	1.1492569512114006	5.526507399302594E-4
56-57	1.9737526426080693E-5	1.9737526426080693E-5	0.0	1.3391319554302967	5.723882663563402E-4
58-59	1.9737526426080693E-5	1.9737526426080693E-5	0.0	1.5471950902508265	6.019945559954612E-4
60-61	1.9737526426080693E-5	1.9737526426080693E-5	0.0	1.7622749157158277	6.414696088476225E-4
62-63	1.9737526426080693E-5	1.9737526426080693E-5	0.0	1.9899768893303076	6.513383720606629E-4
64-65	1.9737526426080693E-5	1.9737526426080693E-5	0.0	2.2171755560209228	6.612071352737033E-4
66-67	1.9737526426080693E-5	1.9737526426080693E-5	0.0	2.4742371001941974	6.710758984867436E-4
68-69	1.9737526426080693E-5	1.9737526426080693E-5	0.0	2.7715631982766773	6.710758984867436E-4
70-71	1.9737526426080693E-5	1.9737526426080693E-5	0.0	3.0924164278590447	7.10550951338905E-4
72-73	1.9737526426080693E-5	1.9737526426080693E-5	0.0	3.3974105549580567	8.487136363214699E-4
74-75	1.9737526426080693E-5	1.9737526426080693E-5	0.0	3.7066581190018892	8.487136363214699E-4
76-77	1.9737526426080693E-5	1.9737526426080693E-5	0.0	4.056624200062726	8.487136363214699E-4
78-79	1.9737526426080693E-5	1.9737526426080693E-5	0.0	4.443913943595281	8.487136363214699E-4
80-81	1.9737526426080693E-5	3.9475052852161386E-5	0.0	4.838980272539713	9.37532505238833E-4
82-83	1.9737526426080693E-5	3.9475052852161386E-5	0.0	5.249826753861795	0.001085563953434438
84-85	1.9737526426080693E-5	3.9475052852161386E-5	0.0	5.67229863824884	0.001085563953434438
86-87	1.9737526426080693E-5	3.9475052852161386E-5	0.0	6.117182483892698	0.0011053014798605189
88	1.9737526426080693E-5	3.9475052852161386E-5	0.0	6.462796440376584	0.0011053014798605189
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8730	0.0	53.995316	2
GTACCTG	10175	0.0	46.761806	1
ACCTGGG	10220	0.0	45.47492	3
CCTGGGG	8860	0.0	35.37681	4
TATAACG	645	0.0	33.517185	2
ATAACGC	735	0.0	31.32819	3
TATCACG	470	0.0	29.998034	2
GGTATCA	19745	0.0	29.359665	1
TAACGCA	805	0.0	27.43649	4
GTATCAA	27635	0.0	26.710707	1
CTGGGGG	5540	0.0	26.380144	5
TCAACGC	32655	0.0	22.420448	4
CAACGCA	33020	0.0	22.20108	5
ATCAACG	33040	0.0	22.18764	3
TATCAAC	33125	0.0	22.07614	2
AACGCAG	33820	0.0	21.88434	6
GTACACG	1210	0.0	21.370884	1
GAGTACT	20850	0.0	20.469088	12-13
CATGGGG	13810	0.0	20.31455	4
ACGCAGA	37370	0.0	19.767693	7
>>END_MODULE