FastQCFastQC Report
Mon 6 Jun 2016
ERR522933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7822434
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172200.22013608552018463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138540.17710600051083844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.1028580106907901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141400.036.6092761
GTATCAA220600.033.9400561
TCAACGC257950.028.5138454
GTACATG267050.028.212831
ATCAACG261950.028.0820353
CAACGCA263450.027.9362285
TATCAAC272900.027.0972142
TACATGG272300.026.984282
AACGCAG275400.026.9386316
ACATGGG276500.025.7543243
CATGGGG202100.024.3709244
ACGCAGA312200.023.748247
CGCAGAG323250.022.9199858
ATGGGGG114650.022.504665
TACCTGG61700.021.7149682
GCAGAGT364100.020.1408759
GAGTACT177300.019.43712412-13
AGAGTAC296600.018.99029210-11
TATCACG8350.018.5791972
CAGAGTA349700.017.47693410-11
Produced by FastQC (version 0.11.5)