FastQCFastQC Report
Mon 6 Jun 2016
ERR522933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7822434
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154950.19808412573375497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131550.16817016289303305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132100.037.8617821
GTATCAA209400.034.018861
TCAACGC246100.028.1078684
CAACGCA250000.027.6693845
ATCAACG251300.027.6022823
GTACATG262450.026.7301671
TATCAAC264000.026.5279052
AACGCAG261900.026.5194856
TACATGG268600.025.7759082
ACATGGG271100.024.6849143
TACCTGG62700.023.6295362
ACGCAGA298600.023.2441717
CATGGGG193700.022.4896054
CGCAGAG312950.022.2133358
ATGGGGG115750.020.908265
GCAGAGT353250.019.4263529
GAGTACT173850.019.23651112-13
AGAGTAC287450.019.11517510-11
GTACCTG83000.018.0268381
GAGTACA200450.017.5341681
Produced by FastQC (version 0.11.5)