FastQCFastQC Report
Mon 6 Jun 2016
ERR522932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7668803
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242470.3161771139511603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191930.25027373893944077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114190.14890198639865962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123200.046.627871
GTATCAA218150.039.8338551
TCAACGC258200.033.2528464
CAACGCA263050.032.727365
ATCAACG263650.032.532583
AACGCAG272650.031.6545896
TATCAAC273600.031.4734152
ACGCAGA309600.027.8313477
CGCAGAG320950.026.830568
GTACATG235250.025.8788661
TACATGG240400.024.772892
GCAGAGT357900.023.7706789
ACATGGG246500.023.4324863
TACCTGG62650.021.7576222
CATGGGG175450.021.7494964
GAGTACT215700.021.008512-13
AGAGTAC312350.020.4392810-11
CAGAGTA347250.020.25872810-11
ATGGGGG105700.019.161355
GTACTTT239850.018.5806914-15
Produced by FastQC (version 0.11.5)