FastQCFastQC Report
Mon 6 Jun 2016
ERR522932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7668803
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210650.2746843281800302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179560.23414345107052562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108410.141364956173734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128200.043.665651
GTATCAA215700.038.274361
TCAACGC262150.030.9948714
ATCAACG267650.030.3589383
CAACGCA268450.030.2849865
TATCAAC274250.029.7505472
AACGCAG278350.029.2918836
ACGCAGA322900.025.221097
GTACATG227300.024.4623971
CGCAGAG333750.024.4337928
TACATGG229900.023.5848122
ACATGGG235300.022.289543
TACCTGG59850.021.6078052
GCAGAGT373400.021.6000949
CATGGGG168550.020.2977224
GAGTACT225200.019.84888512-13
AGAGTAC321750.019.61190210-11
CAGAGTA362300.018.56501410-11
ATGGGGG98600.018.3020175
GTACTTT247550.017.84634414-15
Produced by FastQC (version 0.11.5)