##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522932_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7668803
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.543953860856774	34.0	31.0	34.0	30.0	35.0
2	32.63362861192288	34.0	31.0	34.0	30.0	34.0
3	32.88439134503781	34.0	31.0	34.0	31.0	36.0
4	35.68405173532297	37.0	35.0	37.0	32.0	37.0
5	35.76193611962649	37.0	35.0	37.0	35.0	37.0
6	35.842911859908256	37.0	35.0	37.0	35.0	37.0
7	35.67362077236826	37.0	35.0	37.0	35.0	37.0
8	35.71315014872595	37.0	35.0	37.0	33.0	37.0
9	36.922978983812726	39.0	35.0	39.0	34.0	39.0
10-11	37.03015190245466	38.0	36.0	39.0	33.5	39.0
12-13	36.8984629674279	38.0	36.0	39.0	33.5	39.0
14-15	37.971356677176345	39.0	36.0	41.0	33.5	41.0
16-17	37.99165286942434	39.0	37.0	41.0	34.0	41.0
18-19	37.99141619624341	39.0	37.0	41.0	33.5	41.0
20-21	37.87275902119275	39.0	36.5	41.0	34.0	41.0
22-23	37.782600752685916	39.0	36.5	41.0	34.0	41.0
24-25	37.635605517575556	39.0	36.0	41.0	33.0	41.0
26-27	37.502453577174954	38.0	36.5	41.0	33.0	41.0
28-29	37.461566883384535	38.0	36.5	41.0	33.5	41.0
30-31	37.29942710746384	38.0	36.0	40.5	33.5	41.0
32-33	37.13721737277643	38.0	36.0	40.0	32.5	41.0
34-35	36.94889143716431	38.0	36.0	40.0	31.5	41.0
36-37	36.9585067578343	38.0	35.5	40.0	33.0	41.0
38-39	37.0821285929499	38.0	36.0	40.0	32.0	41.0
40-41	36.990782459791966	38.0	36.5	40.0	32.0	41.0
42-43	37.016142219326795	38.0	36.5	40.0	32.5	41.0
44-45	36.834387843839515	38.0	35.5	40.0	32.0	41.0
46-47	36.57887717287822	38.0	35.5	40.0	31.0	41.0
48-49	36.52342445098668	38.0	36.0	40.0	31.0	41.0
50-51	36.134641221061486	37.0	35.0	40.0	30.0	41.0
52-53	36.16214270988576	37.5	35.0	40.0	29.5	41.0
54-55	35.965852558737	37.5	35.0	40.0	30.0	41.0
56-57	35.65709648559234	37.0	35.0	40.0	28.5	41.0
58-59	35.52817988674373	36.5	35.0	40.0	28.5	41.0
60-61	35.25337670037945	36.5	34.5	40.0	28.0	41.0
62-63	35.04717822325075	37.5	34.5	39.0	27.0	41.0
64-65	34.90604465912085	37.0	34.0	39.0	27.0	41.0
66-67	34.67272571742944	36.5	34.0	39.0	27.5	40.5
68-69	34.21060561863436	36.0	33.5	38.0	26.0	40.0
70-71	33.95116708565861	36.0	33.5	37.5	26.0	40.0
72-73	33.699209315977996	35.5	33.0	37.0	26.0	39.0
74-75	33.40387254699332	35.5	33.0	37.0	25.5	39.0
76-77	31.949059846758352	34.0	30.5	36.0	23.5	37.5
78-79	32.983331231745034	35.0	32.5	37.0	26.0	37.0
80-81	32.99914915013464	35.0	33.0	36.5	25.0	37.0
82-83	32.808306589698546	35.0	32.5	36.0	25.0	37.5
84-85	32.60357268272506	35.0	32.5	36.0	25.0	37.0
86-87	32.35576158365262	35.0	32.5	36.0	25.0	37.0
88-89	32.18909013570958	35.0	32.0	36.0	24.0	36.5
90-91	32.04012777483005	35.0	32.0	35.5	23.0	36.5
92-93	31.878972311585002	35.0	31.5	35.5	23.0	36.5
94-95	31.757762000145263	35.0	32.0	35.0	23.0	36.0
96-97	31.569859337891454	35.0	31.0	35.0	22.5	36.5
98-99	31.424634978887838	34.5	31.0	35.0	22.0	36.5
100	30.206204149461136	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12314054415023179
1101	2	-0.1251028922633992
1101	3	-0.17967460528796408
1101	4	-0.11940588739172853
1101	5	-0.1187296793103414
1101	6	-0.1867469357157745
1101	7	-0.09043284760404902
1101	8	-0.1058872602968961
1101	9	-0.16999166862819237
1101	10-11	-0.09564918952998624
1101	12-13	-0.13230092567117424
1101	14-15	-0.2252449305745401
1101	16-17	-0.08687402954136303
1101	18-19	-0.14386472457632493
1101	20-21	-0.1876952235422209
1101	22-23	-0.06870898961773975
1101	24-25	-0.17133490691303166
1101	26-27	-0.19496134655699393
1101	28-29	-0.29154660635231266
1101	30-31	-0.20899728019670505
1101	32-33	-0.1445270891251056
1101	34-35	-0.06584137579454818
1101	36-37	-0.2154494632147248
1101	38-39	-0.1517162873525919
1101	40-41	-0.1357809677940054
1101	42-43	-0.20100182726029914
1101	44-45	-0.31652165854663394
1101	46-47	-0.203272231482174
1101	48-49	-0.2512353940461409
1101	50-51	-0.2197011827729014
1101	52-53	-0.3578229553977721
1101	54-55	-0.2257131345981307
1101	56-57	-0.3531681157205284
1101	58-59	-0.38592695565156276
1101	60-61	-0.4572299822530681
1101	62-63	-0.3274110333915985
1101	64-65	-0.3059319471560684
1101	66-67	-0.3209677101945232
1101	68-69	-0.37464898057182694
1101	70-71	-0.39790552998979933
1101	72-73	-0.46791807856429557
1101	74-75	-0.4721511046106457
1101	76-77	-0.4471123656076479
1101	78-79	-0.4201425322831085
1101	80-81	-0.30430306825700626
1101	82-83	-0.3004124119206537
1101	84-85	-0.37716652347525326
1101	86-87	-0.4213150226452811
1101	88-89	-0.4727288974566193
1101	90-91	-0.4190953613043966
1101	92-93	-0.7140578621093887
1101	94-95	-0.6327041349062696
1101	96-97	-0.5420158020250163
1101	98-99	-0.6480211525279458
1101	100	-0.6746051115654161
1102	1	-0.0036557724776571376
1102	2	-0.06085672969830114
1102	3	-0.04602838509254781
1102	4	-0.06698034615021697
1102	5	-0.10423802479779454
1102	6	-0.10236236042831592
1102	7	-0.11280545594208746
1102	8	-0.06982896329689936
1102	9	-0.13908578863186705
1102	10-11	-0.12436380807960745
1102	12-13	-0.12389501365018418
1102	14-15	-0.2111316598199906
1102	16-17	-0.075361935971344
1102	18-19	-0.16408458904793832
1102	20-21	-0.1768242743634687
1102	22-23	-0.1645189455306877
1102	24-25	-0.2236018028809994
1102	26-27	-0.160347098099912
1102	28-29	-0.21771343741226445
1102	30-31	-0.18885304320797758
1102	32-33	-0.12322873240578502
1102	34-35	-0.1519446903264665
1102	36-37	-0.2420874052800599
1102	38-39	-0.2480912674867355
1102	40-41	-0.268727519398972
1102	42-43	-0.2336622894937861
1102	44-45	-0.29208071421451365
1102	46-47	-0.2330266839314561
1102	48-49	-0.24324782623303776
1102	50-51	-0.12474727084369164
1102	52-53	-0.23959914619102562
1102	54-55	-0.2951425774998384
1102	56-57	-0.15624470788491607
1102	58-59	-0.4298071719947316
1102	60-61	-0.31710969408455725
1102	62-63	-0.2577761399337959
1102	64-65	-0.3027293220905989
1102	66-67	-0.3626580023847694
1102	68-69	-0.34654941158864716
1102	70-71	-0.34830243526675275
1102	72-73	-0.45050873054287877
1102	74-75	-0.838052914306985
1102	76-77	-0.3559485906159203
1102	78-79	-0.3929058169244044
1102	80-81	-0.3735880130457758
1102	82-83	-0.2809797032067465
1102	84-85	-0.30846226332177906
1102	86-87	-0.38420001720953323
1102	88-89	-0.3770540082162199
1102	90-91	-0.3805832181841211
1102	92-93	-0.44080839375405034
1102	94-95	-0.516510144113326
1102	96-97	-0.5837639145756057
1102	98-99	-0.6726781225946645
1102	100	-0.4783063958882927
1103	1	-0.10197977008691339
1103	2	-0.09863038124809265
1103	3	-0.127052550066324
1103	4	-0.028442796743107124
1103	5	-0.1391330991095927
1103	6	-0.06909553022340731
1103	7	-0.09908706319091465
1103	8	-0.10446041450472876
1103	9	-0.15797224339826954
1103	10-11	-0.12743793267730297
1103	12-13	-0.12379941425265883
1103	14-15	-0.2097210682764583
1103	16-17	-0.17406391358120743
1103	18-19	-0.13733101999800823
1103	20-21	-0.145719258873946
1103	22-23	-0.06923790780577832
1103	24-25	-0.12704537600960464
1103	26-27	-0.17838767938842892
1103	28-29	-0.1382106057028878
1103	30-31	-0.19024630088962624
1103	32-33	-0.1317680968209558
1103	34-35	-0.12118111481512983
1103	36-37	-0.1460299934587752
1103	38-39	-0.2076778214901438
1103	40-41	-0.2294814559371261
1103	42-43	-0.2681799791441861
1103	44-45	-0.35237729046531996
1103	46-47	-0.3500427709977245
1103	48-49	-0.33299910317523995
1103	50-51	-0.20539975433312208
1103	52-53	-0.29909123798108084
1103	54-55	-0.2840646090470855
1103	56-57	-0.17929717386964938
1103	58-59	-0.35192588898316757
1103	60-61	-0.2926034528934238
1103	62-63	-0.23259220427708982
1103	64-65	-0.3477960601335468
1103	66-67	-0.3794450408873047
1103	68-69	-0.24781797763012747
1103	70-71	-0.4271281851059783
1103	72-73	-0.49099780187513886
1103	74-75	-0.4275787820199213
1103	76-77	-0.3212165184537632
1103	78-79	-0.29807897124688765
1103	80-81	-0.29879730842713315
1103	82-83	-0.3346405423596224
1103	84-85	-0.3095504829980129
1103	86-87	-0.30695867207838035
1103	88-89	-0.3428103040232564
1103	90-91	-0.3981396765308389
1103	92-93	-0.34497683982346317
1103	94-95	-0.14940601586904734
1103	96-97	-0.8722805753418008
1103	98-99	-1.186962078965724
1103	100	-0.45502019455347664
1104	1	-0.04510562336847812
1104	2	-0.09353875581149396
1104	3	-0.12809049612091172
1104	4	-0.09981860627019046
1104	5	-0.17713835220752827
1104	6	-0.0882634783855778
1104	7	-0.13918822379136486
1104	8	-0.055956248578254986
1104	9	-0.09972466356985166
1104	10-11	-0.10379711498292465
1104	12-13	-0.10727815154740483
1104	14-15	-0.23602798999217356
1104	16-17	-0.10822839780017546
1104	18-19	-0.1878990895569217
1104	20-21	-0.2455385079354926
1104	22-23	-0.1588052966164284
1104	24-25	-0.22183668733600115
1104	26-27	-0.14368491429384278
1104	28-29	-0.24620627269027295
1104	30-31	-0.2909825646253168
1104	32-33	-0.1532309127612237
1104	34-35	-0.11228433302223806
1104	36-37	-0.21606560200258684
1104	38-39	-0.19049660438503935
1104	40-41	-0.17137803191918266
1104	42-43	-0.20226755027861998
1104	44-45	-0.2864546290110255
1104	46-47	-0.13036292179857867
1104	48-49	-0.1460197272129946
1104	50-51	-0.09259400081921143
1104	52-53	-0.11535937256319784
1104	54-55	-0.2403414320744588
1104	56-57	-0.1800863979555416
1104	58-59	-0.17579610176860427
1104	60-61	-0.2555733144683785
1104	62-63	-0.2138910801333651
1104	64-65	-0.2151132997412759
1104	66-67	-0.33971650913093043
1104	68-69	-0.2158640076374141
1104	70-71	-0.28121324298514594
1104	72-73	-0.22311208876776334
1104	74-75	-0.21535461494269725
1104	76-77	-0.19940836172600385
1104	78-79	-0.20989761815128105
1104	80-81	-0.0215863076555749
1104	82-83	-0.08390616166244769
1104	84-85	-0.0896544519813105
1104	86-87	-0.12293686024726469
1104	88-89	-0.14094826350390122
1104	90-91	-0.15972334323637938
1104	92-93	-0.2176089707852391
1104	94-95	-0.3451036958254683
1104	96-97	-0.08948373153232936
1104	98-99	-0.8686307846249015
1104	100	-0.33505328966903036
1105	1	-0.1197645960170064
1105	2	-1.1169041359753002
1105	3	-0.1687855969424561
1105	4	-0.13541266832221055
1105	5	-0.14162233218804943
1105	6	-0.15388533573998586
1105	7	-0.1394498443251848
1105	8	-0.168876744743784
1105	9	-0.2066678935038837
1105	10-11	-0.14248869917794593
1105	12-13	-0.09698907165830661
1105	14-15	-0.19851527079122633
1105	16-17	-0.663442686515225
1105	18-19	-0.1859223674767847
1105	20-21	-0.2700243338969557
1105	22-23	-0.1912384230536972
1105	24-25	-0.2432505760147876
1105	26-27	-0.24512422815437418
1105	28-29	-0.2974542168072034
1105	30-31	-0.2585895392811679
1105	32-33	-0.26003382775210326
1105	34-35	-0.1971057055068215
1105	36-37	-0.32028374621178557
1105	38-39	-0.3449935008749563
1105	40-41	-0.2931964171531476
1105	42-43	-0.3098397651237761
1105	44-45	-0.38797895788022174
1105	46-47	-0.33847101374640687
1105	48-49	-0.27926517858168864
1105	50-51	-0.18190069050586288
1105	52-53	-0.2539961614356372
1105	54-55	-0.3027493004394586
1105	56-57	-0.1553899397279821
1105	58-59	-0.29902487726923965
1105	60-61	-0.21087768677670482
1105	62-63	-0.1256376122615137
1105	64-65	-0.16617738540101357
1105	66-67	-0.25731717008957844
1105	68-69	-0.28858042319657784
1105	70-71	-0.3956589372657149
1105	72-73	-0.3716725804933958
1105	74-75	-0.32106615116398984
1105	76-77	-0.2035096208830467
1105	78-79	-0.2413179694914689
1105	80-81	-0.15420280003498732
1105	82-83	-0.09598819503688816
1105	84-85	-0.11230185754255473
1105	86-87	-0.21915012292387814
1105	88-89	-0.15244098102960635
1105	90-91	-0.21287764890742267
1105	92-93	-0.21215197324990598
1105	94-95	-0.2327713231038402
1105	96-97	-0.2630443299695564
1105	98-99	-0.18871195525124307
1105	100	-0.1812980430661426
1106	1	-0.10629054584325104
1106	2	-0.16445814421120986
1106	3	-0.07279145993562253
1106	4	-0.07914782691066335
1106	5	-0.0846237881809202
1106	6	-0.02881560432135899
1106	7	-0.07514529897876798
1106	8	-0.025599671805281332
1106	9	-0.10540513412308172
1106	10-11	-0.08423924601006405
1106	12-13	-0.051617996025036916
1106	14-15	-0.1031447792999245
1106	16-17	-0.017805587052109217
1106	18-19	-0.1321862182985214
1106	20-21	-0.09828156655085252
1106	22-23	-0.04233024694119791
1106	24-25	0.020120344782824873
1106	26-27	-0.008639696613997216
1106	28-29	-0.056393554315143035
1106	30-31	-0.027394950028416076
1106	32-33	0.11275670598981691
1106	34-35	0.08008236221936471
1106	36-37	0.020826697686906925
1106	38-39	0.0015501564837023807
1106	40-41	0.01007184783751569
1106	42-43	0.012350785501631378
1106	44-45	0.010762036125839813
1106	46-47	-0.01774220441759411
1106	48-49	-0.05785860226580297
1106	50-51	0.055887162354139264
1106	52-53	-0.002784421910071444
1106	54-55	-0.027567587061980703
1106	56-57	-0.10944211900780232
1106	58-59	-0.2249210841860858
1106	60-61	-0.06110710240429995
1106	62-63	-0.015981265430582425
1106	64-65	0.015287888136938932
1106	66-67	0.031729461363227074
1106	68-69	0.061802424799317635
1106	70-71	0.001141631815364974
1106	72-73	-0.01599664548035662
1106	74-75	-0.05252324079695114
1106	76-77	-0.09871697868450369
1106	78-79	0.011951758812855928
1106	80-81	0.06203413680503189
1106	82-83	0.06704564118772538
1106	84-85	0.09008072818966895
1106	86-87	0.12310776684324765
1106	88-89	0.12989106112173943
1106	90-91	0.13540179771012362
1106	92-93	0.034617117257628394
1106	94-95	0.009942055375297798
1106	96-97	-0.0692842629252759
1106	98-99	-0.01848668052234359
1106	100	0.028252006834460275
1107	1	-0.04772179396420029
1107	2	-0.11276165726556542
1107	3	-0.09647369669713868
1107	4	-0.046228849704277764
1107	5	-0.07242435718612228
1107	6	-0.16914450428174632
1107	7	-0.07102917785399399
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2316	66-67	-0.2583942067877416
2316	68-69	-0.0013632291735419244
2316	70-71	0.018937754733777012
2316	72-73	0.058396654974984585
2316	74-75	-0.06150496092442381
2316	76-77	-0.19524515368297912
2316	78-79	-0.1697248401553324
2316	80-81	-0.12307157612712416
2316	82-83	-0.14282128582474485
2316	84-85	0.007042187434144864
2316	86-87	-0.1981658177502581
2316	88-89	-0.10203005970432599
2316	90-91	-0.10043837612364115
2316	92-93	-0.25863242894732963
2316	94-95	-0.16383922222659564
2316	96-97	-0.0042443215653626964
2316	98-99	0.10682486442059158
2316	100	-0.21568222003620363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	36.0
13	165.0
14	684.0
15	1806.0
16	4175.0
17	8216.0
18	13671.0
19	20282.0
20	28172.0
21	38310.0
22	49123.0
23	56471.0
24	60938.0
25	67459.0
26	76473.0
27	91490.0
28	110638.0
29	135088.0
30	168246.0
31	210660.0
32	269126.0
33	361249.0
34	547432.0
35	1031928.0
36	1557676.0
37	1258412.0
38	1260074.0
39	240684.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65697607607322	18.96952054709647	13.30167355180826	24.071829825022053
2	16.360778791031592	20.864073819708214	38.94124799643387	23.833899392826325
3	19.099856205770106	25.482092747865092	29.944956033026642	25.473095013338167
4	13.402706185398971	16.94294623853895	37.271153955989476	32.3831936200726
5	13.857809426220422	37.05677915952833	34.478973864389424	14.606437549861814
6	31.338525157509906	36.43119768034421	17.90840301463451	14.321874147511371
7	27.313871539013324	32.259610789323965	22.257919521468995	18.168598150193713
8	25.851097247970568	34.80185954031523	20.53910291041192	18.80794030130228
9	26.50515341181668	15.638229329922806	20.287898906778544	37.56871835148197
10-11	24.931521246734498	25.74538369878372	28.573296063713734	20.74979899076805
12-13	25.489881459848835	23.550971639086928	28.384260941159717	22.574885959904517
14-15	22.91412525485845	25.093186186404715	26.08061025704025	25.91207830169659
16-17	22.639538180867685	27.87082427678739	26.63988817424663	22.849749368098298
18-19	22.464597092292642	26.953642366834618	28.50366504967993	22.07809549119281
20-21	23.232373086844678	26.22020263334019	28.398392051405846	22.149032228409286
22-23	22.987683772256	26.070320210967672	28.227762779509284	22.71423323726704
24-25	22.821874274449264	26.158150940894032	28.330592880046122	22.689381904610578
26-27	22.71249001477074	26.29235812334874	28.297965305163146	22.697186556717377
28-29	22.653552973800263	26.508300287630632	28.308710763770435	22.529435974798677
30-31	23.06169349301469	26.261893388137565	28.259703514114005	22.416709604733736
32-33	22.84908347330753	26.345245485180126	28.204778771217825	22.60089227029452
34-35	23.061000261655384	26.441414394384495	28.113785470099796	22.383799873860326
36-37	23.03561977811455	26.356996632916722	28.03678917139863	22.570594417570096
38-39	22.862177880763813	26.50164414627787	28.034276796995844	22.60190117596248
40-41	22.978375784070415	26.327102933531616	28.110437487216466	22.584083795181503
42-43	22.829967367244016	26.478198645789526	27.858808725105703	22.83302526186075
44-45	22.772958176192137	26.55177344465345	27.88843507093498	22.786833308219432
46-47	22.793889217117076	26.45432693875262	27.672401313086652	23.07938253104365
48-49	22.871284416951315	26.52756421041374	27.60371548523157	22.997435887403373
50-51	22.840114302720668	26.787095076247674	27.456225842686514	22.916564778345137
52-53	22.876525674748805	26.790707421703814	27.287101265087376	23.045665638460004
54-55	22.756226275344876	26.614821788801507	27.50261778056544	23.126334155288177
56-57	22.82116805478012	26.767157565411203	27.267482167427353	23.144192212381327
58-59	22.772339201136692	26.67610432243504	27.401741471781378	23.14981500464689
60-61	22.849609682032032	26.669612027975802	27.47366828081247	23.007110009179694
62-63	22.726804167066838	26.720944135596785	27.431794719725396	23.120456977610985
64-65	22.64181489790355	26.521493716802947	27.388618008312832	23.448073376980673
66-67	22.523245783614858	26.57282722584776	27.284906818002515	23.619020172534867
68-69	22.45406059068033	26.60073702840377	27.334609552105633	23.610592828810265
70-71	22.480761420621466	26.417305094734544	27.34111028619255	23.760823198451433
72-73	22.40407503897141	26.567671764355783	27.184355048913456	23.843898147759347
74-75	22.364401322985486	26.66113086243461	27.101693506188656	23.872774308391243
76-77	22.33742665666271	26.480482865792755	27.180318507340708	24.001771970203826
78-79	22.21585198729813	26.453890387882755	27.203739412663037	24.12651821215608
80-81	22.20185319001017	26.57485193368199	26.991274292581092	24.23202058372675
82-83	22.24468073661696	26.49558612060768	27.057480025154174	24.20225311762119
84-85	22.160391089197027	26.532825844257395	27.014763122973385	24.29201994357219
86-87	22.02596942979581	26.504115621025655	27.079121140207562	24.390793808970976
88-89	21.872369590704892	26.35229525236347	27.126329149280565	24.64900600765108
90-91	22.081004339052452	26.251801961864587	27.031214445686864	24.635979253396098
92-93	21.92759108066617	26.210235032200043	27.074772920174123	24.78740096695967
94-95	21.742218922241644	26.0748843485468	27.148542940345287	25.03435378886627
96-97	21.65697705692277	26.009663159924884	27.240772919784217	25.092586863368126
98-99	21.626621340347793	25.942599630607628	27.155881507815145	25.274897521229434
100	21.633217476872936	25.885931570835467	27.253753294202166	25.22709765808943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1766.0
1	1275.0
2	1526.0
3	2147.5
4	1576.5
5	1297.0
6	1925.0
7	3427.5
8	5294.5
9	6271.5
10	5660.5
11	4968.5
12	5202.5
13	5651.5
14	6249.0
15	7012.5
16	7675.5
17	8211.5
18	8968.5
19	9951.5
20	11168.5
21	12814.5
22	15070.0
23	18252.0
24	22848.5
25	28562.5
26	35929.0
27	44703.0
28	54261.5
29	64956.5
30	76469.0
31	88950.0
32	102813.5
33	115977.0
34	127805.0
35	140808.5
36	158599.5
37	175341.5
38	188335.0
39	200595.0
40	210740.5
41	221211.5
42	233886.0
43	247940.5
44	259446.0
45	269960.5
46	280581.5
47	290899.5
48	304680.0
49	314559.0
50	315823.0
51	312139.5
52	303693.5
53	293622.5
54	281861.0
55	264106.5
56	239739.0
57	215285.5
58	193024.0
59	167670.5
60	140663.5
61	116207.0
62	94968.5
63	74550.5
64	56240.0
65	42686.5
66	32272.0
67	25087.0
68	19581.5
69	14966.0
70	11840.0
71	9310.0
72	7377.5
73	6020.0
74	4799.5
75	3717.5
76	2750.0
77	1860.0
78	1224.0
79	799.0
80	532.5
81	361.5
82	249.0
83	153.5
84	94.5
85	60.5
86	37.5
87	28.0
88	21.5
89	15.5
90	8.0
91	5.0
92	5.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029913403695465902
2	0.07291881144945306
3	0.0026862080040392223
4	1.303984467980205E-5
5	0.0014213430700984234
6	0.001030147729704362
7	0.0
8	3.390359616748533E-4
9	0.0
10-11	1.303984467980205E-5
12-13	1.1735860211821845E-4
14-15	0.010705712482117482
16-17	0.0011279465648028773
18-19	1.5647813615762458E-4
20-21	3.325160393349523E-4
22-23	0.001825578255172287
24-25	7.758707584482221E-4
26-27	0.007843466574900933
28-29	0.008280301371674303
30-31	0.010197158539605204
32-33	0.009427807703496882
34-35	0.004166230375196755
36-37	0.0038663139475613078
38-39	0.0030448037327337786
40-41	0.0032273615582510077
42-43	0.0016691001190146625
44-45	0.005313736707019336
46-47	0.017903706745368217
48-49	0.018686097426156338
50-51	0.014265590079703443
52-53	0.007791307196181725
54-55	0.0012127055552215906
56-57	0.002366731809384072
58-59	0.0030252439657140755
60-61	0.0032273615582510077
62-63	0.003175202179531799
64-65	0.0038402342582017036
66-67	0.0027122876933988265
68-69	0.007530510302585683
70-71	4.237949520935666E-4
72-73	0.00470738392940854
74-75	0.007048036049433009
76-77	0.0065525219516005305
78-79	0.004250989365615468
80-81	3.455558840147543E-4
82-83	0.0012453051669210958
84-85	8.671496712068363E-4
86-87	0.0012257453999013927
88-89	0.001069267263743768
90-91	0.0010171078850245599
92-93	3.1295627231524916E-4
94-95	9.258289722659454E-4
96-97	0.002523209945541697
98-99	0.00343599907312784
100	0.005294176939999632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7668803.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.24295084573799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82287855647401	42.996622870555996
2	15.27718527863523	17.79576702489567
3	4.941289657298727	8.633858718738097
4	2.1603417817153736	5.032987208097701
5	1.110343187676352	3.233483185086689
6	0.6619100433891073	2.3130956472847246
7	0.4260953948193947	1.7371937195242924
8	0.304947004212456	1.4208810701520904
9	0.2109063266630206	1.1055426135200517
>10	1.017323098110129	10.29318165900984
>50	0.04577785911955653	1.8363604713799924
>100	0.019822924986941336	1.9886417637764024
>500	6.952439328742771E-4	0.2891197604666887
>1k	3.9295991050586663E-4	0.4454190478545057
>5k	0.0	0.0
>10k+	9.068305626528538E-5	0.8778452396572952
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21065	0.2746843281800302	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17956	0.23414345107052562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10841	0.141364956173734	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.303984467980205E-5
2	0.0	0.0	0.0	0.0	1.303984467980205E-5
3	0.0	0.0	0.0	0.0	1.303984467980205E-5
4	0.0	0.0	0.0	1.303984467980205E-5	1.303984467980205E-5
5	0.0	0.0	0.0	1.303984467980205E-5	1.303984467980205E-5
6	0.0	0.0	0.0	1.303984467980205E-5	3.911953403940615E-5
7	0.0	0.0	0.0	1.303984467980205E-5	3.911953403940615E-5
8	0.0	0.0	0.0	1.303984467980205E-5	3.911953403940615E-5
9	0.0	0.0	0.0	2.60796893596041E-5	5.21593787192082E-5
10-11	0.0	0.0	0.0	2.60796893596041E-5	5.21593787192082E-5
12-13	0.0	0.0	0.0	3.2599611699505126E-5	5.21593787192082E-5
14-15	0.0	0.0	0.0	3.911953403940615E-5	7.171914573891128E-5
16-17	0.0	0.0	0.0	3.911953403940615E-5	1.043187574384164E-4
18-19	0.0	0.0	0.0	5.21593787192082E-5	1.1735860211821845E-4
20-21	0.0	0.0	0.0	5.867930105910923E-5	1.1735860211821845E-4
22-23	0.0	0.0	0.0	2.738367382758431E-4	1.6299805849752564E-4
24-25	0.0	0.0	0.0	7.69350836108321E-4	2.2167735955663486E-4
26-27	0.0	0.0	0.0	0.0017342993424136728	2.2167735955663486E-4
28-29	0.0	0.0	0.0	0.0053463363187188405	2.2167735955663486E-4
30-31	0.0	0.0	0.0	0.017584230550713065	2.347172042364369E-4
32-33	0.0	0.0	0.0	0.04939493164709016	2.60796893596041E-4
34-35	0.0	0.0	1.303984467980205E-5	0.1024214600375052	2.7383673827584303E-4
36-37	0.0	0.0	1.303984467980205E-5	0.18213403056513514	2.8035666061574407E-4
38-39	0.0	6.519922339901025E-6	1.303984467980205E-5	0.2901952234266547	2.9991642763544713E-4
40-41	0.0	1.303984467980205E-5	1.303984467980205E-5	0.4065367176598486	2.9991642763544713E-4
42-43	0.0	2.60796893596041E-5	1.303984467980205E-5	0.5276181954341506	2.9991642763544713E-4
44-45	0.0	2.60796893596041E-5	1.303984467980205E-5	0.6801322188091153	2.9991642763544713E-4
46-47	0.0	2.60796893596041E-5	1.303984467980205E-5	0.8604797906531176	3.325160393349523E-4
48-49	0.0	3.2599611699505126E-5	1.303984467980205E-5	1.046160658971159	3.651156510344574E-4
50-51	1.303984467980205E-5	3.911953403940615E-5	1.303984467980205E-5	1.2376833777057517	3.651156510344574E-4
52-53	1.303984467980205E-5	3.911953403940615E-5	1.303984467980205E-5	1.4281107494872407	3.911953403940615E-4
54-55	1.303984467980205E-5	5.21593787192082E-5	1.303984467980205E-5	1.6508247766959196	4.172750297536656E-4
56-57	1.303984467980205E-5	5.21593787192082E-5	1.303984467980205E-5	1.9125800988759263	4.172750297536656E-4
58-59	1.303984467980205E-5	6.519922339901025E-5	1.303984467980205E-5	2.1976180637317193	4.3031487443346764E-4
60-61	1.303984467980205E-5	6.519922339901025E-5	1.303984467980205E-5	2.4954024767620187	4.368347967733687E-4
62-63	1.303984467980205E-5	6.519922339901025E-5	1.303984467980205E-5	2.796870645914362	4.694344084728738E-4
64-65	1.303984467980205E-5	6.519922339901025E-5	1.303984467980205E-5	3.1067821144968777	4.7595433081277486E-4
66-67	1.303984467980205E-5	7.82390680788123E-5	1.303984467980205E-5	3.4621961210895624	4.889941754925768E-4
68-69	1.303984467980205E-5	7.82390680788123E-5	1.303984467980205E-5	3.867402774592071	4.955140978324779E-4
70-71	1.303984467980205E-5	8.475899041871333E-5	1.303984467980205E-5	4.292958105717411	5.541933988915871E-4
72-73	1.303984467980205E-5	9.127891275861435E-5	1.303984467980205E-5	4.705929986726742	6.911117680295086E-4
74-75	1.303984467980205E-5	9.127891275861435E-5	1.303984467980205E-5	5.12964669975223	6.911117680295086E-4
76-77	1.303984467980205E-5	9.127891275861435E-5	1.303984467980205E-5	5.587488686304759	6.976316903694096E-4
78-79	1.303984467980205E-5	9.127891275861435E-5	1.303984467980205E-5	6.095253718213911	7.041516127093107E-4
80-81	1.303984467980205E-5	9.127891275861435E-5	1.303984467980205E-5	6.622187321802373	7.171914573891127E-4
82-83	1.303984467980205E-5	9.779883509851538E-5	1.303984467980205E-5	7.158718251075168	7.69350836108321E-4
84-85	1.303984467980205E-5	1.043187574384164E-4	1.303984467980205E-5	7.693117165742816	7.69350836108321E-4
86-87	1.303984467980205E-5	1.043187574384164E-4	1.303984467980205E-5	8.24425272105699	7.69350836108321E-4
88	1.303984467980205E-5	1.043187574384164E-4	1.303984467980205E-5	8.683441209795062	7.69350836108321E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12820	0.0	43.66565	1
GTATCAA	21570	0.0	38.27436	1
TCAACGC	26215	0.0	30.994871	4
ATCAACG	26765	0.0	30.358938	3
CAACGCA	26845	0.0	30.284986	5
TATCAAC	27425	0.0	29.750547	2
AACGCAG	27835	0.0	29.291883	6
ACGCAGA	32290	0.0	25.22109	7
GTACATG	22730	0.0	24.462397	1
CGCAGAG	33375	0.0	24.433792	8
TACATGG	22990	0.0	23.584812	2
ACATGGG	23530	0.0	22.28954	3
TACCTGG	5985	0.0	21.607805	2
GCAGAGT	37340	0.0	21.600094	9
CATGGGG	16855	0.0	20.297722	4
GAGTACT	22520	0.0	19.848885	12-13
AGAGTAC	32175	0.0	19.611902	10-11
CAGAGTA	36230	0.0	18.565014	10-11
ATGGGGG	9860	0.0	18.302017	5
GTACTTT	24755	0.0	17.846344	14-15
>>END_MODULE