FastQCFastQC Report
Mon 6 Jun 2016
ERR522931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014027
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315930.39422128225921876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260900.32555418143712267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160720.20054836351312516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123030.1535183248072411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG143750.053.2858622
GTACCTG165150.047.1044271
ACCTGGG165250.046.0679323
CCTGGGG147500.034.7443584
TATAACG7850.030.5491622
CTGGGGG92150.025.4075075
GGTATCA291050.025.1447221
GTATCAA394250.024.825911
TAACGCA10250.022.9348534
GTACATG366950.022.8405021
TATCACG6600.022.7984222
CATGGGG211950.022.737374
TACATGG376500.021.8935262
TCAACGC455250.021.1819294
ATGGGGG126050.021.1106515
ACATGGG382550.020.9078523
CAACGCA460500.020.8883485
ATCAACG463200.020.8103123
ATAACGC11750.020.8093623
TATCAAC466250.020.7147832
Produced by FastQC (version 0.11.5)