FastQCFastQC Report
Mon 6 Jun 2016
ERR522931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014027
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280920.3505353800280433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252190.31468573789431953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156430.19519524952935646No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108720.1356621334068378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG138850.055.7821922
GTACCTG159600.049.0500071
ACCTGGG160950.047.47563
CCTGGGG145750.034.338554
TATAACG9250.028.4531612
CTGGGGG86350.027.0479875
GGTATCA288300.026.97421
GTATCAA390550.024.9201951
TATCACG6250.024.8152242
GTACCCG14750.023.9071031
TGGGGGG124200.022.2482916
ATAACGC12250.021.8665183
GTACATG362650.021.5088141
CATGGGG205650.021.388884
TCAACGC451850.021.2062474
CAACGCA455600.021.0523325
ATCAACG458300.020.887293
TAACGCA11950.020.8424554
TATCAAC463150.020.741662
AACGCAG470250.020.616336
Produced by FastQC (version 0.11.5)