##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522931_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8014027
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24007480883206	33.0	31.0	34.0	30.0	34.0
2	32.37341476388837	34.0	31.0	34.0	30.0	34.0
3	32.56714096421187	34.0	31.0	34.0	30.0	35.0
4	35.658532969754155	37.0	35.0	37.0	33.0	37.0
5	35.59005815677936	36.0	35.0	37.0	33.0	37.0
6	35.7128074312702	37.0	35.0	37.0	33.0	37.0
7	35.55473222139132	37.0	35.0	37.0	33.0	37.0
8	35.54305806556429	36.0	35.0	37.0	33.0	37.0
9	36.840789530656686	38.0	37.0	39.0	33.0	39.0
10-11	36.643866435688324	37.5	35.0	39.0	33.0	39.0
12-13	36.647182558781	37.5	35.0	39.0	33.0	39.0
14-15	37.83120583446998	38.0	37.0	41.0	33.0	41.0
16-17	37.64674551258687	38.0	36.5	41.0	33.0	41.0
18-19	37.55179636904143	38.0	36.0	41.0	33.0	41.0
20-21	37.56714608024156	38.0	36.5	41.0	33.0	41.0
22-23	37.42358286289777	38.0	36.0	41.0	33.0	41.0
24-25	37.3485446205759	38.0	36.5	41.0	32.0	41.0
26-27	37.04058409086967	38.0	36.0	40.0	32.5	41.0
28-29	37.00877773434006	38.0	36.0	40.0	32.0	41.0
30-31	36.771186134012275	38.0	35.5	40.0	32.0	41.0
32-33	36.72561054511047	37.5	36.0	40.0	31.5	41.0
34-35	36.500517218122674	38.0	35.5	40.0	30.5	41.0
36-37	36.28493995590482	37.0	35.5	40.0	30.5	41.0
38-39	36.244535050855205	37.0	35.5	40.0	30.0	41.0
40-41	36.43122084564976	37.5	35.5	40.0	31.0	41.0
42-43	36.47876648032256	37.5	35.5	40.0	31.0	41.0
44-45	36.30052625228241	37.0	35.0	40.0	30.5	41.0
46-47	36.19764620209041	37.0	35.5	40.0	30.0	41.0
48-49	35.98540271950668	38.0	35.0	40.0	30.0	41.0
50-51	35.82483419384536	38.0	34.5	40.0	29.5	41.0
52-53	35.83702606691991	37.5	35.0	40.0	28.5	41.0
54-55	35.64032664227361	37.5	34.5	40.0	28.0	41.0
56-57	35.39757802163631	37.0	35.0	40.0	28.0	41.0
58-59	35.21333369603072	37.0	34.5	40.0	27.5	41.0
60-61	35.032618869389886	37.0	34.0	39.0	27.5	41.0
62-63	34.78724684855691	36.5	34.0	39.0	26.0	41.0
64-65	34.60214770926028	37.0	33.5	39.0	26.0	41.0
66-67	34.10222251559672	36.0	33.5	38.0	26.0	40.0
68-69	33.96522759406725	36.5	33.5	37.5	26.0	40.0
70-71	33.54940649688353	35.0	33.0	37.0	25.5	39.5
72-73	33.318191902772476	35.0	33.0	37.0	25.5	39.0
74-75	33.13000505239127	35.0	32.5	37.0	24.5	39.0
76-77	31.520119972143842	34.0	30.0	36.0	23.0	37.0
78-79	32.634994678705226	35.0	32.0	36.5	24.0	37.0
80-81	32.74760298411772	35.0	32.5	36.0	25.0	37.5
82-83	32.645370797977094	35.0	32.5	37.0	24.5	37.0
84-85	32.313087602524924	35.0	32.0	36.0	24.0	36.5
86-87	32.19191837012778	35.0	32.0	36.0	24.0	36.5
88-89	32.14976609137953	35.0	32.0	36.0	23.5	37.0
90-91	31.917553933871197	35.0	32.0	35.0	23.5	36.5
92-93	31.70669926617417	35.0	31.5	35.0	22.0	36.5
94-95	31.84488796955638	35.0	31.0	35.0	22.0	37.0
96-97	31.57132662517858	35.0	31.0	35.0	21.0	37.0
98-99	31.42359197192622	34.0	31.0	35.0	20.5	36.0
100	30.20359314986086	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05972482901874798
1101	2	-0.14111684318239526
1101	3	-0.11658892246641273
1101	4	-0.06791389010835047
1101	5	-0.1601078043720463
1101	6	-0.10355330236811966
1101	7	-0.06138462987583182
1101	8	-0.14678369079948084
1101	9	-0.14130655348873944
1101	10-11	-0.13406699541995692
1101	12-13	-0.14905054619917024
1101	14-15	-0.3022875493084811
1101	16-17	-0.31772388931606343
1101	18-19	-0.4134653602108429
1101	20-21	-0.302940361195013
1101	22-23	-0.808892695957482
1101	24-25	-0.24452840839128243
1101	26-27	-0.252186190762167
1101	28-29	-0.3724604472271409
1101	30-31	-0.3440575597143365
1101	32-33	-0.27833453066211433
1101	34-35	-0.3435294380582121
1101	36-37	-0.40362232387517594
1101	38-39	-0.28562173471002694
1101	40-41	-0.29673326676130785
1101	42-43	-0.22845659926609585
1101	44-45	-0.3838132737054778
1101	46-47	-0.22747115899046833
1101	48-49	-0.35900383653837054
1101	50-51	-0.3224190469483972
1101	52-53	-0.30098058499183367
1101	54-55	-0.2937495488400472
1101	56-57	-0.47681416927231624
1101	58-59	-0.42779058183553076
1101	60-61	-0.6153848101191315
1101	62-63	-0.512985222739907
1101	64-65	-0.4882701667991327
1101	66-67	-0.42921295893414424
1101	68-69	-0.4912708881929504
1101	70-71	-0.5275756656408745
1101	72-73	-0.586897559246772
1101	74-75	-0.4801935291568782
1101	76-77	-0.5529164903372035
1101	78-79	-0.48405732774845234
1101	80-81	-0.35064365007561094
1101	82-83	-0.3467295247446458
1101	84-85	-0.3837288083649639
1101	86-87	-0.423217368638543
1101	88-89	-0.46708055639504664
1101	90-91	-0.4635197693189923
1101	92-93	-0.44299284710414
1101	94-95	-0.606847203188952
1101	96-97	-0.6716603365614979
1101	98-99	-0.653772079904499
1101	100	-0.6327848065251196
1102	1	-0.04781313861577274
1102	2	-0.11900610362365427
1102	3	-0.1315752279691651
1102	4	-0.15608843963404695
1102	5	-0.15172210794651875
1102	6	-0.16937350007451357
1102	7	-0.1210284883118149
1102	8	-0.21869188239784876
1102	9	-0.15310947035089129
1102	10-11	-0.2685847585342458
1102	12-13	-0.18389849394213087
1102	14-15	-0.3189877487287802
1102	16-17	-0.34252285941548166
1102	18-19	-0.28235756649911536
1102	20-21	-0.31529831540512276
1102	22-23	-0.257972289201291
1102	24-25	-0.2472334616521792
1102	26-27	-0.2261521118014329
1102	28-29	-0.35989154654444633
1102	30-31	-0.4051860894562864
1102	32-33	-0.4076217928789916
1102	34-35	-0.3652123987272091
1102	36-37	-0.38403638152797726
1102	38-39	-0.3617490163587078
1102	40-41	-0.3159178246251244
1102	42-43	-0.36404530718819217
1102	44-45	-0.2775295597094498
1102	46-47	-0.27355413834673215
1102	48-49	-0.292233689130704
1102	50-51	-0.1606570530595306
1102	52-53	-0.27793186836945694
1102	54-55	-0.2576103870586195
1102	56-57	-0.29410975969125985
1102	58-59	-0.2709562718502738
1102	60-61	-0.33140743212484836
1102	62-63	-0.36616344250938226
1102	64-65	-0.34948014741470246
1102	66-67	-0.46178155255200437
1102	68-69	-0.327581492363727
1102	70-71	-0.36297263149023706
1102	72-73	-0.36284562552607014
1102	74-75	-0.2692115722831403
1102	76-77	-0.2973031340384651
1102	78-79	-0.38557803099244126
1102	80-81	-0.39767113483515715
1102	82-83	-0.341130538020046
1102	84-85	-0.4248274766944924
1102	86-87	-0.3777707217230599
1102	88-89	-0.4141912168588959
1102	90-91	-0.41990689526620883
1102	92-93	-0.4071583010226476
1102	94-95	-0.580263378609434
1102	96-97	-0.6342056621490677
1102	98-99	-0.6165758008685813
1102	100	-0.6636530447009825
1103	1	-0.045004540910127844
1103	2	-0.08883179325351165
1103	3	-0.1488169438369482
1103	4	-0.04394284689803385
1103	5	-0.13646688726802836
1103	6	-0.1602800236259725
1103	7	-0.15631965116762814
1103	8	-0.14840759206749254
1103	9	-0.2049176526873424
1103	10-11	-0.2539082065196041
1103	12-13	-0.207760412981699
1103	14-15	-0.16381117347874863
1103	16-17	-0.27195747956775307
1103	18-19	-0.24806040891359515
1103	20-21	-0.24770294261664816
1103	22-23	-0.29604525411546234
1103	24-25	-0.26548065383659036
1103	26-27	-0.29573915315592814
1103	28-29	-0.2339786533034598
1103	30-31	-0.46738509262236505
1103	32-33	-0.34272956238160646
1103	34-35	-0.4145255388694409
1103	36-37	-0.432905898506327
1103	38-39	-0.3513989796611341
1103	40-41	-0.3824261772740343
1103	42-43	-0.36387156320096636
1103	44-45	-0.27818103041164477
1103	46-47	-0.4366356087810601
1103	48-49	-0.4419341758745787
1103	50-51	-0.41890801863160476
1103	52-53	-0.33825533101950356
1103	54-55	-1.1403393955815417
1103	56-57	-0.37179305413926755
1103	58-59	-0.3779092282419825
1103	60-61	-0.3062264975513216
1103	62-63	-0.35898679582602
1103	64-65	-0.3168618442653184
1103	66-67	-0.31314586894249885
1103	68-69	-0.31031119664471163
1103	70-71	-0.44325225225744447
1103	72-73	-0.4544017247759129
1103	74-75	-0.46894493400296966
1103	76-77	-0.40437625126988763
1103	78-79	-0.32751297132377033
1103	80-81	-1.2129532098196911
1103	82-83	-0.22189193621545655
1103	84-85	-0.2514230340625332
1103	86-87	-0.17234954596297314
1103	88-89	-0.18689280860344581
1103	90-91	-0.2615336783214133
1103	92-93	-0.2377732951010465
1103	94-95	-0.2321396735422887
1103	96-97	-0.39681772726524756
1103	98-99	-0.2891691395016238
1103	100	-0.230975035081336
1104	1	-0.0698138051892414
1104	2	-0.19070743070746232
1104	3	-0.14455190981752963
1104	4	-0.02727541510523679
1104	5	-0.10536223443708792
1104	6	-0.194315406356246
1104	7	-0.17411209869992916
1104	8	-0.11224111869411502
1104	9	-0.21342722392055435
1104	10-11	-0.15402458484254566
1104	12-13	-0.11573842108003163
1104	14-15	-0.32487262339672185
1104	16-17	-0.27492181565362017
1104	18-19	-0.2513942481536091
1104	20-21	-0.33849427695638923
1104	22-23	-0.28796849857477724
1104	24-25	-0.15191840729698924
1104	26-27	-0.277828927317735
1104	28-29	-0.37252428803132176
1104	30-31	-0.3666358807847345
1104	32-33	-0.2097815096309077
1104	34-35	-0.27118472346001
1104	36-37	-0.3390802866478282
1104	38-39	-0.21346096061622433
1104	40-41	-0.13329544465718612
1104	42-43	-0.19508294123336611
1104	44-45	-0.32099587272409735
1104	46-47	-0.3014058476699262
1104	48-49	-0.3670212452495534
1104	50-51	-0.22828074283482636
1104	52-53	-0.2806904399137693
1104	54-55	-0.26737031076671514
1104	56-57	-0.2320138101775413
1104	58-59	-0.2212015851134339
1104	60-61	-0.3587157661626392
1104	62-63	-0.3595317090927068
1104	64-65	-0.5246849176200072
1104	66-67	-0.3490222127529776
1104	68-69	-0.24656420182599703
1104	70-71	-0.21058299398983849
1104	72-73	-0.24407851933803215
1104	74-75	-0.30410944997263556
1104	76-77	-0.1850281027860774
1104	78-79	-0.16090851237974846
1104	80-81	-0.21227186005276621
1104	82-83	-0.19015588118336524
1104	84-85	-0.8497471918186115
1104	86-87	-0.2213784739308977
1104	88-89	-0.30750651693301023
1104	90-91	-0.3098797147273018
1104	92-93	-0.30039829181133726
1104	94-95	-0.2717388094510511
1104	96-97	-0.1998407125776609
1104	98-99	-0.30341962194333405
1104	100	-1.4961525321413731
1105	1	-0.017898172687758063
1105	2	-0.13874702330215882
1105	3	-0.12061007396155787
1105	4	-0.06564958237887453
1105	5	-0.07657071681101968
1105	6	-0.09112889031852944
1105	7	-0.14669731286871723
1105	8	-0.2011172280792053
1105	9	-0.17811367221692365
1105	10-11	-0.12433797925503143
1105	12-13	-0.10673567398528405
1105	14-15	-0.17618908759205
1105	16-17	-0.1935901810646996
1105	18-19	-0.24806505553389968
1105	20-21	-0.2134748326824294
1105	22-23	-0.26618275768328914
1105	24-25	-0.12628805577925561
1105	26-27	-0.20698347388113092
1105	28-29	-0.15594308449734484
1105	30-31	-0.19380060263801369
1105	32-33	-0.1670272663402912
1105	34-35	-0.23052551625372075
1105	36-37	-0.2996357917029471
1105	38-39	-0.22392181113868048
1105	40-41	-0.15194737652058876
1105	42-43	-0.1871745979704258
1105	44-45	-0.33778800561910316
1105	46-47	-0.29272571630190214
1105	48-49	-0.24692539993603901
1105	50-51	-0.21498725241106342
1105	52-53	-0.2853687660039981
1105	54-55	-1.1355170485482873
1105	56-57	-0.20589327478549535
1105	58-59	-0.23073909799104086
1105	60-61	-0.217103215371111
1105	62-63	-0.19868102741409643
1105	64-65	-0.23474543804334758
1105	66-67	-0.30695715558971415
1105	68-69	-0.09501176713271065
1105	70-71	-0.11031082722802665
1105	72-73	-0.18687054627863375
1105	74-75	-0.22018574409279523
1105	76-77	-0.24944801978266895
1105	78-79	-0.23747914652748392
1105	80-81	-0.10509017294982925
1105	82-83	-0.08450853064179853
1105	84-85	-0.13603669650395744
1105	86-87	-0.09724371999794101
1105	88-89	-0.23100463735202936
1105	90-91	-0.2187711566220223
1105	92-93	-0.12595656924358778
1105	94-95	-0.14212781458742896
1105	96-97	-0.19350950257834043
1105	98-99	-0.04120403425535102
1105	100	-0.045796947996784354
1106	1	-0.004189125944463967
1106	2	-0.10326278457446136
1106	3	-0.08702042806891086
1106	4	-0.047450815639301425
1106	5	-0.1247459523975607
1106	6	-0.13882835259217785
1106	7	-0.0764884976367668
1106	8	-0.1659806313859633
1106	9	-0.1335912406177755
1106	10-11	-0.1148483267620577
1106	12-13	-0.15556425408571073
1106	14-15	-0.17066731702529125
1106	16-17	-0.19739818015666089
1106	18-19	-0.22006718363817157
1106	20-21	-0.20897423715324948
1106	22-23	-0.1390991555867842
1106	24-25	-0.10634095337123739
1106	26-27	-0.06687998381011795
1106	28-29	-0.08693545312340945
1106	30-31	-0.14574537293174927
1106	32-33	-0.009019648967040439
1106	34-35	-0.07044911000981102
1106	36-37	-0.12198989955423656
1106	38-39	-0.1714538652093367
1106	40-41	-0.11938071825433383
1106	42-43	-0.07870722056892987
1106	44-45	-0.04970351447903454
1106	46-47	-0.204361136437484
1106	48-49	-0.23406520728659785
1106	50-51	-0.06217457708996932
1106	52-53	-0.15471086376678045
1106	54-55	-0.19596969822811872
1106	56-57	-0.12131450542579358
1106	58-59	-0.15598387248499535
1106	60-61	-0.15007936870262029
1106	62-63	-0.25225784017792563
1106	64-65	-0.16887702299101193
1106	66-67	-0.13910886852364257
1106	68-69	-0.10475499455085213
1106	70-71	-0.1716400823615345
1106	72-73	-0.14164558025529317
1106	74-75	-0.15708202701066654
1106	76-77	-0.1912831072019543
1106	78-79	-0.01899375324320829
1106	80-81	-0.020798230144635
1106	82-83	-0.01503437640824501
1106	84-85	-0.007240885509219197
1106	86-87	0.00411249645666345
1106	88-89	-0.10782428901496388
1106	90-91	-0.1026412787580071
1106	92-93	0.03363918844854297
1106	94-95	0.05641360144195673
1106	96-97	0.062181526282071076
1106	98-99	-0.05712943960021377
1106	100	-0.07253763329036289
1107	1	0.04207280672541458
1107	2	-0.012313020016783582
1107	3	-0.056647182204102364
1107	4	0.025611473392906703
1107	5	-0.11964887143216885
1107	6	-0.102906548262645
1107	7	-0.1376468941579887
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2316	90-91	0.12364309813118268
2316	92-93	0.18946828660088144
2316	94-95	0.1559238815622379
2316	96-97	0.20508504553364304
2316	98-99	0.24307905846550426
2316	100	0.15746293343001128
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	1.0
11	5.0
12	48.0
13	251.0
14	954.0
15	2526.0
16	5415.0
17	10382.0
18	17054.0
19	24991.0
20	33664.0
21	44400.0
22	56748.0
23	66710.0
24	75276.0
25	83255.0
26	95911.0
27	113737.0
28	137714.0
29	167359.0
30	206833.0
31	255133.0
32	319582.0
33	412445.0
34	587150.0
35	1046106.0
36	1648146.0
37	1204803.0
38	1162781.0
39	234504.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20289942277698	18.184972027690925	12.825191981146562	23.786936568385535
2	16.548927455857154	20.408613798000548	39.06854823110397	23.97391051503833
3	19.22360630928745	25.91638386044869	29.651547218395947	25.208462611867915
4	13.303343345801503	16.30782000716745	36.281808054097276	34.10702859293377
5	13.77090824647807	36.52763793574822	33.682667700523695	16.01878611725002
6	31.152977148691914	35.135531737040566	18.774955462465996	14.936535651801522
7	28.281602779926097	32.02515190242707	21.614154982776448	18.079090334870386
8	27.096514406759358	34.0812895806547	20.217429064279383	18.604766948306565
9	27.67764570795681	15.611851569753881	20.03537048228063	36.67513224000867
10-11	25.5744583840309	25.461575060827723	28.036522711989864	20.927443843151515
12-13	25.78166455754605	23.298355185395163	28.10235233862825	22.81762791843053
14-15	23.35683952399574	24.696712983303186	26.059923532965918	25.886523959735158
16-17	22.88008742799005	27.355830509441116	26.7468892679659	23.017192794602934
18-19	22.621560551286336	26.48643711355617	28.605573195127214	22.286429140030275
20-21	23.36923586706232	25.82775655822327	28.51597626668096	22.28703130803346
22-23	23.183971240221762	25.564763781246995	28.376397715489325	22.874867263041914
24-25	22.867519092287818	25.634026678133782	28.41187118240815	23.086583047170254
26-27	22.812273231273352	25.667756581374846	28.476863591596675	23.04310659575513
28-29	22.81398388387223	26.06943220906813	28.514263193646	22.602320713413647
30-31	23.48095049063108	25.740208312456453	28.409499465323123	22.36934173158934
32-33	23.21607065710176	25.833541108699336	28.325048524595235	22.625339709603665
34-35	23.24930094334431	25.987074736634685	28.263434323366692	22.500189996654314
36-37	23.30241513822717	25.89790257087309	28.162609407483757	22.637072883415986
38-39	23.233781213778865	26.15229266200536	27.98359211593484	22.630334008280936
40-41	23.18355704957613	25.89646018118757	28.235953146498677	22.68402962273762
42-43	23.00396326262263	26.055099576981195	27.90568908726035	23.035248073135826
44-45	22.85371005218297	26.159113843576637	27.994019046879814	22.993157057360584
46-47	22.89504963197533	26.031177951017735	27.844157169960283	23.229615247046656
48-49	22.94204125156106	26.144341088086666	27.750255395600625	23.16336226475165
50-51	22.863001644029694	26.471629973133666	27.62566443548054	23.039703947356106
52-53	22.955246338229838	26.57230533232557	27.207271943555916	23.26517638588868
54-55	22.792381791655725	26.353779576692304	27.553060085754165	23.300778545897806
56-57	22.965044345794247	26.40014475166043	27.243728727901196	23.391082174644126
58-59	22.916936792693587	26.31890241581584	27.566831622933808	23.19732916855677
60-61	23.248387852723898	26.418981597599956	27.544446199739802	22.788184349936348
62-63	22.973414007850124	26.540896143985783	27.46953820329851	23.016151644865584
64-65	22.868911188192865	26.30371192819767	27.394434141643586	23.432942741965874
66-67	22.813972413224267	26.28409703406504	27.27128830438578	23.630642248324904
68-69	22.604509184871688	26.305660186419537	27.41766133490674	23.672169293802032
70-71	22.68100829909018	26.222955022148085	27.464224843215213	23.63181183554652
72-73	22.613763651223238	26.507717035240415	27.095097810382384	23.783421503153967
74-75	22.6770657401915	26.542794725740254	26.96372921603197	23.816410318036276
76-77	22.57847848120158	26.27395958719167	27.226989674301105	23.920572257305643
78-79	22.493892061283162	26.25079067218335	27.250041834342	24.005275432191493
80-81	22.57731370232614	26.484477676472707	26.899537654155576	24.038670967045576
82-83	22.61882412499046	26.475134532503958	26.940429105202796	23.965612237302793
84-85	22.55594063819623	26.618595425747305	26.738154819775893	24.087309116280572
86-87	22.368749918112	26.54637293884012	26.9507405482534	24.13413659479448
88-89	22.157274169122417	26.30185893793639	27.048725847223952	24.49214104571724
90-91	22.623889301570042	26.159344616020814	26.9709816172695	24.245784465139643
92-93	22.454744895657722	26.27682568180339	26.880551505864343	24.387877916674544
94-95	22.22595040893314	26.097232800900024	26.938308395382894	24.738508394783942
96-97	22.117843629476724	25.97519367173152	27.115726990007655	24.7912357087841
98-99	22.173092902330758	25.973880449418687	26.917722829194968	24.935303819055584
100	22.01513373002984	26.031832467579623	27.04669155388825	24.90634224850228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4037.0
1	2990.5
2	5132.5
3	7139.0
4	4293.0
5	2878.5
6	4115.5
7	7172.0
8	10748.0
9	12387.5
10	10562.0
11	8272.0
12	8210.5
13	8766.0
14	9544.5
15	10261.0
16	10788.0
17	11481.0
18	12218.0
19	13101.5
20	14155.5
21	15465.0
22	17219.0
23	19766.0
24	23297.5
25	27764.5
26	33726.0
27	41031.5
28	49012.0
29	58062.0
30	69096.0
31	80201.0
32	92489.5
33	106683.5
34	120570.0
35	134048.0
36	152019.0
37	169807.5
38	181761.0
39	193627.0
40	201423.5
41	209321.0
42	220126.0
43	232823.0
44	248072.0
45	262989.5
46	278682.0
47	295665.0
48	319087.0
49	338443.5
50	353450.0
51	352921.0
52	334023.0
53	322102.0
54	311659.5
55	293444.0
56	267850.0
57	242440.5
58	220467.0
59	193749.5
60	162278.5
61	133707.0
62	109286.0
63	85364.0
64	63990.0
65	48420.0
66	36245.0
67	27831.5
68	21510.0
69	16080.5
70	12460.0
71	9638.5
72	7510.5
73	5846.0
74	4407.0
75	3078.0
76	2089.0
77	1326.0
78	822.0
79	528.0
80	336.5
81	203.5
82	135.0
83	99.5
84	67.0
85	49.0
86	36.5
87	23.0
88	14.0
89	8.0
90	5.0
91	3.0
92	1.5
93	1.0
94	2.0
95	1.5
96	0.5
97	0.0
98	0.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044983626833301156
2	0.010444187422877413
3	0.0
4	2.370843023114347E-4
5	2.994749081828649E-4
6	0.0
7	1.2478121174286037E-5
8	1.3725933291714641E-4
9	0.0
10-11	0.0
12-13	2.932358475957219E-4
14-15	0.012871181991276046
16-17	0.0014661792379786092
18-19	8.110778763285922E-5
20-21	7.674044522185912E-4
22-23	7.11252906934304E-4
24-25	0.004810315712687267
26-27	0.01647735901064471
28-29	0.013226808444743198
30-31	0.009683022031245965
32-33	0.007050138463471611
34-35	0.009084072214880235
36-37	0.005078595317934417
38-39	0.003974281594010103
40-41	0.005652588891951574
42-43	0.0069066400699673205
44-45	0.007343374311067332
46-47	0.015585173346683258
48-49	0.018186861611521898
50-51	0.017444413401651877
52-53	0.011585935510324585
54-55	0.0038120660187443843
56-57	0.0037683925946343827
58-59	0.009882671970034541
60-61	0.0022585399325457728
62-63	1.6845463585286148E-4
64-65	3.1195302935715095E-4
66-67	9.79532512181454E-4
68-69	8.110778763285922E-5
70-71	1.996499387885766E-4
72-73	0.0028762069306729316
74-75	0.0053468749231815665
76-77	0.005353113983768709
78-79	0.0034439614441029464
80-81	5.615154528428716E-5
82-83	8.921856639614516E-4
84-85	2.3084524172429168E-4
86-87	3.992998775771532E-4
88-89	1.9341087820143356E-4
90-91	4.741686046228694E-4
92-93	5.178420287328705E-4
94-95	3.743436352285811E-5
96-97	0.0018030885096843323
98-99	3.7434363522858107E-4
100	9.98249693942883E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8014027.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.5882641443917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.61494756752072	36.429025676556286
2	16.543337357192208	17.06884114825229
3	5.704110853820881	8.8279553230741
4	2.50999261078844	5.179446472233015
5	1.3692060700733106	3.531748220552322
6	0.763303317960195	2.3626495913532684
7	0.512365836386419	1.8502444874245336
8	0.33414615319693225	1.3790416011164572
9	0.25116543217019577	1.1661469792862722
>10	1.2697165973519478	11.622260137266105
>50	0.08127471935084189	2.8736983005831545
>100	0.042351740078658745	3.9774437157441196
>500	0.002823213292975294	0.9949445280899185
>1k	9.524016989051327E-4	0.8205695741116903
>5k	1.7007173192100622E-4	0.5152446250724927
>10k+	1.3605738553680496E-4	1.4007396192840242
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28092	0.3505353800280433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25219	0.31468573789431953	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15643	0.19519524952935646	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10872	0.1356621334068378	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2478121174286037E-5
2	0.0	0.0	0.0	0.0	1.2478121174286037E-5
3	0.0	0.0	0.0	0.0	1.2478121174286037E-5
4	0.0	0.0	0.0	0.0	1.2478121174286037E-5
5	0.0	0.0	0.0	0.0	1.2478121174286037E-5
6	0.0	0.0	0.0	0.0	6.239060587143018E-5
7	0.0	0.0	0.0	0.0	6.239060587143018E-5
8	0.0	0.0	0.0	0.0	7.486872704571622E-5
9	0.0	0.0	0.0	1.2478121174286037E-5	1.2478121174286036E-4
10-11	0.0	0.0	0.0	1.2478121174286037E-5	1.2478121174286036E-4
12-13	0.0	3.119530293571509E-5	0.0	1.2478121174286037E-5	1.2478121174286036E-4
14-15	0.0	3.743436352285811E-5	0.0	1.8717181761429056E-5	1.9341087820143356E-4
16-17	0.0	3.743436352285811E-5	0.0	3.743436352285811E-5	2.6204054466000677E-4
18-19	0.0	3.743436352285811E-5	0.0	4.991248469714415E-5	2.745186658342928E-4
20-21	0.0	3.743436352285811E-5	0.0	8.734684822000225E-5	2.807577264214358E-4
22-23	0.0	3.743436352285811E-5	0.0	2.7451866583429283E-4	3.3067021111858E-4
24-25	0.0	3.743436352285811E-5	0.0	8.297950580900214E-4	3.7434363522858107E-4
26-27	0.0	3.743436352285811E-5	0.0	0.0018093275702714753	3.992998775771532E-4
28-29	0.0	3.743436352285811E-5	0.0	0.005796087285455864	3.992998775771532E-4
30-31	0.0	3.743436352285811E-5	0.0	0.016046863830131843	4.055389381642962E-4
32-33	0.0	3.743436352285811E-5	0.0	0.039574361304248164	4.117779987514392E-4
34-35	0.0	4.367342411000113E-5	0.0	0.07798825733928773	4.866467257971554E-4
36-37	0.0	4.991248469714415E-5	0.0	0.13589921770914923	4.866467257971554E-4
38-39	0.0	4.991248469714415E-5	0.0	0.22247866147693288	4.991248469714414E-4
40-41	0.0	4.991248469714415E-5	0.0	0.3225407151735326	5.427982710814426E-4
42-43	0.0	4.991248469714415E-5	0.0	0.4188343762754979	5.490373316685855E-4
44-45	0.0	4.991248469714415E-5	0.0	0.5333772895948565	5.677545134300146E-4
46-47	0.0	4.991248469714415E-5	0.0	0.6706116662696544	5.989498163657297E-4
48-49	0.0	4.991248469714415E-5	0.0	0.8143533831368424	6.363841798885879E-4
50-51	0.0	4.991248469714415E-5	0.0	0.9681587047310921	6.363841798885879E-4
52-53	0.0	4.991248469714415E-5	0.0	1.1249899707101063	6.6134042223716E-4
54-55	0.0	8.734684822000225E-5	0.0	1.3064031354024637	6.925357251728751E-4
56-57	0.0	8.734684822000225E-5	0.0	1.5114124272353961	7.05013846347161E-4
58-59	0.0	8.734684822000225E-5	0.0	1.7433183092595022	7.11252906934304E-4
60-61	0.0	8.734684822000225E-5	0.0	1.9763472221892937	7.362091492828761E-4
62-63	0.0	8.734684822000225E-5	0.0	2.220076124026036	7.861216339800203E-4
64-65	0.0	8.734684822000225E-5	0.0	2.4668496874293036	7.861216339800203E-4
66-67	0.0	8.734684822000225E-5	0.0	2.7463845579756594	8.110778763285923E-4
68-69	0.0	8.734684822000225E-5	0.0	3.068170346818148	8.360341186771644E-4
70-71	0.0	8.734684822000225E-5	0.0	3.415392785674418	8.921856639614516E-4
72-73	0.0	8.734684822000225E-5	0.0	3.737347029152759	0.0010855965421628852
74-75	0.0	8.734684822000225E-5	0.0	4.068591233845357	0.0010855965421628852
76-77	0.0	8.734684822000225E-5	0.0	4.436614700699161	0.0011105527845114572
78-79	0.0	9.358590880714527E-5	0.0	4.853334035435617	0.0011355090268600292
80-81	0.0	9.98249693942883E-5	0.0	5.283086767738617	0.0011854215115571734
82-83	0.0	9.98249693942883E-5	0.0	5.714218332431373	0.0012977246021257478
84-85	0.0	9.98249693942883E-5	0.0	6.148881205416453	0.0012977246021257478
86-87	0.0	9.98249693942883E-5	0.0	6.611819500982465	0.0012977246021257478
88	0.0	9.98249693942883E-5	0.0	6.981708446951826	0.0013226808444743198
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	13885	0.0	55.782192	2
GTACCTG	15960	0.0	49.050007	1
ACCTGGG	16095	0.0	47.4756	3
CCTGGGG	14575	0.0	34.33855	4
TATAACG	925	0.0	28.453161	2
CTGGGGG	8635	0.0	27.047987	5
GGTATCA	28830	0.0	26.9742	1
GTATCAA	39055	0.0	24.920195	1
TATCACG	625	0.0	24.815224	2
GTACCCG	1475	0.0	23.907103	1
TGGGGGG	12420	0.0	22.248291	6
ATAACGC	1225	0.0	21.866518	3
GTACATG	36265	0.0	21.508814	1
CATGGGG	20565	0.0	21.38888	4
TCAACGC	45185	0.0	21.206247	4
CAACGCA	45560	0.0	21.052332	5
ATCAACG	45830	0.0	20.88729	3
TAACGCA	1195	0.0	20.842455	4
TATCAAC	46315	0.0	20.74166	2
AACGCAG	47025	0.0	20.61633	6
>>END_MODULE