##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522930_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800992
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.530389918444946	33.0	31.0	34.0	30.0	34.0
2	31.79715901014552	34.0	31.0	34.0	28.0	35.0
3	31.813994731792256	34.0	31.0	34.0	28.0	36.0
4	34.03255372594659	35.0	34.0	37.0	32.0	37.0
5	34.14048424423873	35.0	34.0	37.0	32.0	37.0
6	34.50768132229349	35.0	35.0	37.0	32.0	37.0
7	34.3940472806098	35.0	34.0	37.0	32.0	37.0
8	34.605149273289385	36.0	35.0	37.0	32.0	37.0
9	35.67839335210817	37.0	35.0	39.0	32.0	39.0
10-11	35.678263146088376	37.0	34.5	39.0	32.0	39.0
12-13	35.783026243314794	37.5	35.5	39.0	31.5	39.0
14-15	36.37501998898385	38.0	36.0	40.0	31.0	41.0
16-17	36.51588568966436	38.0	36.0	40.0	31.0	41.0
18-19	36.49301079627227	38.0	35.5	40.0	31.0	41.0
20-21	36.715435715427944	38.5	36.0	40.5	31.0	41.0
22-23	36.600431040226724	38.0	35.5	40.5	31.5	41.0
24-25	36.610790608731186	38.0	36.0	40.5	31.0	41.0
26-27	36.38688900339369	38.0	36.5	40.0	30.0	41.0
28-29	36.19104804463318	38.0	35.0	40.0	30.0	41.0
30-31	35.9523693053606	37.0	35.5	40.0	30.0	41.0
32-33	35.67319677155701	37.5	34.5	40.0	29.0	41.0
34-35	35.52386351521828	37.5	35.0	40.0	29.0	41.0
36-37	35.41649546472166	37.5	35.0	40.0	28.0	41.0
38-39	35.2091464037597	36.5	35.0	40.0	27.5	41.0
40-41	35.112254524173345	37.0	35.0	40.0	27.0	41.0
42-43	34.74943142445941	36.0	35.0	40.0	25.0	41.0
44-45	34.45149645306587	37.0	34.0	40.0	23.5	41.0
46-47	33.93369348670066	36.5	33.5	39.5	20.0	41.0
48-49	33.61538141202182	37.0	33.0	39.5	19.5	41.0
50-51	32.59658066221282	36.0	31.5	38.5	17.0	40.0
52-53	32.81617380865656	36.5	33.0	38.5	15.5	39.5
54-55	33.63439371190988	37.5	33.0	39.5	16.0	40.5
56-57	33.64744290924113	37.0	33.5	40.0	14.5	41.0
58-59	33.59973142579201	37.5	33.0	40.0	13.0	41.0
60-61	33.19519159441019	36.5	33.0	39.5	13.0	41.0
62-63	32.903475140367085	37.0	32.0	39.0	11.0	41.0
64-65	32.536174785895774	36.0	32.0	39.0	10.0	40.5
66-67	32.17585391828503	36.0	31.5	38.0	10.0	40.0
68-69	31.7528731388035	35.5	31.0	37.5	10.0	40.0
70-71	31.28762787397168	35.0	30.5	37.0	9.5	39.0
72-73	30.761721595653952	35.0	29.5	37.0	9.0	39.0
74-75	30.283406034007925	34.5	29.0	36.0	9.0	38.0
76-77	29.922557956948168	34.5	29.0	36.5	9.0	38.0
78-79	29.52455369041062	34.0	27.0	36.0	8.5	37.0
80-81	29.150100888843482	34.0	26.0	35.5	7.5	37.5
82-83	28.73087776070077	34.0	26.0	35.0	7.5	37.0
84-85	28.392076699952028	34.0	25.0	35.0	7.0	36.5
86-87	28.08393790755317	34.0	24.5	35.0	7.0	37.0
88-89	27.762514769638067	33.5	23.5	35.0	7.0	36.5
90-91	27.406324125815107	33.0	21.0	35.0	7.0	36.0
92-93	26.918802526607557	33.0	19.0	35.0	7.0	35.0
94-95	26.709872392548107	33.0	19.0	35.0	7.0	36.0
96-97	26.444711581173042	33.0	17.5	35.0	7.0	35.0
98-99	26.256171043513795	32.0	17.5	35.0	7.0	36.0
100	24.87696613866136	30.0	15.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08810464405799934
1101	2	-0.15143163866875398
1101	3	-0.05629963992891973
1101	4	-0.11419684519043471
1101	5	-0.19850158232505066
1101	6	-0.027291649221027114
1101	7	-0.15851252451377462
1101	8	-0.01868661946531347
1101	9	-0.0032056243226179504
1101	10-11	-0.13549051005546175
1101	12-13	-0.14181637387325452
1101	14-15	-0.32117437876313915
1101	16-17	-0.17128148265582155
1101	18-19	-0.10702243973137371
1101	20-21	-0.10221799729806946
1101	22-23	-0.10467448406651414
1101	24-25	-0.12934577727141772
1101	26-27	0.03946972049381259
1101	28-29	-0.09011455670317758
1101	30-31	-0.18690740561061858
1101	32-33	-0.22075139101549013
1101	34-35	-0.26656945886082895
1101	36-37	-0.19693604391954267
1101	38-39	-0.19806912351359074
1101	40-41	-0.21787311824912337
1101	42-43	-0.19293550542594318
1101	44-45	-0.1990982477801424
1101	46-47	-0.290557628785173
1101	48-49	-0.39121266657565457
1101	50-51	-0.40908967391087003
1101	52-53	-0.4119881616871126
1101	54-55	-0.3075107375342654
1101	56-57	-0.31216681631216403
1101	58-59	-0.2225794045806495
1101	60-61	-0.18628454481542178
1101	62-63	-0.43819243454312584
1101	64-65	-0.39810305337068996
1101	66-67	-0.5225165548600366
1101	68-69	-0.2778850043965022
1101	70-71	-0.21961156750234423
1101	72-73	-0.2736306696306201
1101	74-75	-0.17731415362229086
1101	76-77	-0.21937512578779206
1101	78-79	-0.39977367498778804
1101	80-81	-0.3197147991139637
1101	82-83	-0.4038607541593926
1101	84-85	-0.37460664952654454
1101	86-87	-0.4039898195380438
1101	88-89	-0.6523572490054832
1101	90-91	-0.5166335166175955
1101	92-93	-0.5268469210972881
1101	94-95	-0.4193699796056123
1101	96-97	-0.47807788691823205
1101	98-99	-0.46082045561977125
1101	100	-0.2773700690253804
1102	1	0.07634072205208753
1102	2	-0.009601074100661577
1102	3	0.01756640515513297
1102	4	0.15461738093073052
1102	5	-0.09301459429696735
1102	6	0.0762777404259154
1102	7	0.09620163038634644
1102	8	-0.08001655943615305
1102	9	0.04997946843431578
1102	10-11	0.1101371324714151
1102	12-13	0.18534081832182636
1102	14-15	0.040475384681251114
1102	16-17	0.03157007940257728
1102	18-19	0.14095256507322773
1102	20-21	0.06384520996647325
1102	22-23	0.07817053526491691
1102	24-25	-0.00988042263583111
1102	26-27	0.017804179609541393
1102	28-29	0.04766672403209071
1102	30-31	-0.05095063424148094
1102	32-33	-0.1447494211318201
1102	34-35	-0.010272437426678493
1102	36-37	-0.4670081198999583
1102	38-39	-0.058919063314853304
1102	40-41	-0.6633143001228063
1102	42-43	-0.8301112659649661
1102	44-45	-0.26436318582914
1102	46-47	-0.14171836224888779
1102	48-49	-0.049394749395553106
1102	50-51	-0.24238314559767815
1102	52-53	-0.058861783899025966
1102	54-55	0.18456115191484201
1102	56-57	0.31009244524994983
1102	58-59	0.3140254217756606
1102	60-61	0.34666278411275186
1102	62-63	-0.01612578068871784
1102	64-65	0.290170274347858
1102	66-67	0.0899989187599104
1102	68-69	0.13567416135634858
1102	70-71	0.1296504680064352
1102	72-73	0.1593097350292716
1102	74-75	-0.011544874481010936
1102	76-77	-0.0845615652442504
1102	78-79	-0.05532577233451974
1102	80-81	-0.11933053755184986
1102	82-83	-0.24623367263955842
1102	84-85	-0.3038575850093217
1102	86-87	-0.38193461192303957
1102	88-89	-0.36005191279692994
1102	90-91	-0.13264556908376335
1102	92-93	-0.07787648207041897
1102	94-95	-0.2172991641246469
1102	96-97	-0.6074124435389656
1102	98-99	-0.451323433055137
1102	100	-0.6020991746925404
1103	1	0.04485260521609291
1103	2	-0.0749698150295437
1103	3	0.05057393418271161
1103	4	0.1620330195374109
1103	5	9.168280542652951E-4
1103	6	0.0751551896276581
1103	7	0.2515218408362756
1103	8	0.17420151023003427
1103	9	0.11306909107582186
1103	10-11	0.07500356026531563
1103	12-13	0.08941375010702046
1103	14-15	0.10869585819358463
1103	16-17	-0.04192298119249216
1103	18-19	0.07292485280907357
1103	20-21	-0.14541413328461772
1103	22-23	0.12127535202834139
1103	24-25	-0.08308434135862086
1103	26-27	-0.21493234978088083
1103	28-29	-0.19226433547443378
1103	30-31	-0.09744011174510803
1103	32-33	-0.22511385327985778
1103	34-35	-0.06052642871840419
1103	36-37	-0.28220110792608466
1103	38-39	-0.22866235068784846
1103	40-41	-0.14573900984704125
1103	42-43	-0.04491532982563484
1103	44-45	-0.002440335683999706
1103	46-47	0.11914997047970388
1103	48-49	0.194521932389641
1103	50-51	0.11403240229782341
1103	52-53	0.10967958039704939
1103	54-55	0.1613334215084521
1103	56-57	0.42743302035879793
1103	58-59	0.25243820298030073
1103	60-61	0.25122895791537303
1103	62-63	0.2870763238105596
1103	64-65	0.12995166041463335
1103	66-67	0.30727486215614164
1103	68-69	0.1598713674521406
1103	70-71	0.12828413092370639
1103	72-73	0.16113529743855892
1103	74-75	0.2713209183346592
1103	76-77	0.2277910290953713
1103	78-79	0.04620838042309927
1103	80-81	0.002216768326231744
1103	82-83	0.08934665210644965
1103	84-85	-0.020283111134144605
1103	86-87	-0.7607501507474232
1103	88-89	-0.17218339864598775
1103	90-91	-0.4845425665438512
1103	92-93	-0.28384187575691655
1103	94-95	-0.6667560309606415
1103	96-97	-0.44545330892358237
1103	98-99	-0.34190874691580575
1103	100	-0.4234548532121316
1104	1	0.008640055207003172
1104	2	0.0720498135392873
1104	3	-0.027134796950221585
1104	4	0.08711883059396541
1104	5	0.0023091266540120614
1104	6	0.14447603205355364
1104	7	0.011128701174520472
1104	8	-0.03088691493817919
1104	9	0.05411519036660195
1104	10-11	-0.01977290477378091
1104	12-13	0.0620678690377332
1104	14-15	-0.08648096372858305
1104	16-17	-0.04277185712447107
1104	18-19	-0.04962796929494573
1104	20-21	-0.03239500480671609
1104	22-23	-0.14126457000293868
1104	24-25	0.04653865827767589
1104	26-27	-0.030273065138217703
1104	28-29	-0.009088985410791395
1104	30-31	0.06704149854767394
1104	32-33	0.19750631061357637
1104	34-35	0.08080892771003079
1104	36-37	0.15285881774221366
1104	38-39	0.15421976424213568
1104	40-41	0.255919246199511
1104	42-43	0.18950797441150513
1104	44-45	0.08092638440147226
1104	46-47	0.03865877321656086
1104	48-49	0.025839984535487304
1104	50-51	-0.05388022954432614
1104	52-53	0.06321658712554523
1104	54-55	0.27929734108474946
1104	56-57	0.2842912275328331
1104	58-59	0.09191540906322615
1104	60-61	-0.01642837182640733
1104	62-63	-0.07016476547840256
1104	64-65	-0.05007823138387835
1104	66-67	0.0010913515178430089
1104	68-69	-0.11840650125126118
1104	70-71	-0.025659341753144105
1104	72-73	0.05835950865878203
1104	74-75	0.03637834354833558
1104	76-77	0.09712096408355109
1104	78-79	0.029407740534029614
1104	80-81	0.13076215726058393
1104	82-83	0.30576881409044887
1104	84-85	0.07519589641268354
1104	86-87	-0.017118496128404104
1104	88-89	0.08030071778427228
1104	90-91	-0.13586340328689772
1104	92-93	-0.1131695091902678
1104	94-95	0.09173628014137591
1104	96-97	0.06298501508987542
1104	98-99	-0.25975531561011067
1104	100	-0.14463285148454474
1105	1	-0.021809609883622016
1105	2	-0.026514508761735556
1105	3	-0.03852417835290112
1105	4	0.1538421363465261
1105	5	0.11919496195739754
1105	6	0.15953464119461103
1105	7	0.038069205508641346
1105	8	0.03513455866308135
1105	9	0.10728205403878377
1105	10-11	0.043853720521724426
1105	12-13	0.1379580877138551
1105	14-15	0.12611261389158557
1105	16-17	0.08440135997560816
1105	18-19	0.0036250842905545255
1105	20-21	0.08306254050455664
1105	22-23	0.16976404504828224
1105	24-25	0.08086686946759158
1105	26-27	0.09955972642994482
1105	28-29	-0.0502816075542043
1105	30-31	-0.13783808183058
1105	32-33	-0.031192467952223524
1105	34-35	-0.08427553840502355
1105	36-37	-0.01707690920813576
1105	38-39	-0.14488386065936254
1105	40-41	-0.1440696722401995
1105	42-43	-0.1281818896546909
1105	44-45	-0.1401384304133444
1105	46-47	-0.0752165715430877
1105	48-49	0.03516696444091849
1105	50-51	0.016253734206649995
1105	52-53	0.06414005048252136
1105	54-55	0.1371947997135834
1105	56-57	0.144435780330312
1105	58-59	0.17107468802303316
1105	60-61	0.17120091304357743
1105	62-63	0.09728376683050044
1105	64-65	-0.019376152975681293
1105	66-67	-0.08769041562163693
1105	68-69	-0.02159675932848515
1105	70-71	0.11736666297498388
1105	72-73	-0.04296904729078577
1105	74-75	-0.050427212007218
1105	76-77	0.12970075131759273
1105	78-79	0.03179643241740138
1105	80-81	0.008126223454436143
1105	82-83	-0.14432722609867454
1105	84-85	-0.1044177265187578
1105	86-87	-0.2957951115736357
1105	88-89	-0.17030261160894966
1105	90-91	-0.3068026270851618
1105	92-93	-0.44753712148341407
1105	94-95	-0.5223360701344291
1105	96-97	-0.3924240176130418
1105	98-99	-0.3162343345226404
1105	100	-0.39681222500700386
1106	1	0.11190304015810071
1106	2	-0.01571972892991269
1106	3	0.025972700458318343
1106	4	0.2765503894436421
1106	5	0.08115196336955677
1106	6	0.2022594229521033
1106	7	0.11815698392830853
1106	8	-1.1104119302001294
1106	9	0.25146452723988943
1106	10-11	0.26933303161358424
1106	12-13	0.14789484151705068
1106	14-15	0.09634698387682761
1106	16-17	0.04320056483471291
1106	18-19	0.23615465133593716
1106	20-21	0.05652942701845376
1106	22-23	0.10459268055120674
1106	24-25	0.37831455545514103
1106	26-27	0.31736143465897015
1106	28-29	0.15830697723942677
1106	30-31	0.20215535885881053
1106	32-33	0.20658153872342666
1106	34-35	0.04896522239477008
1106	36-37	0.20434947005240645
1106	38-39	0.2900004387180033
1106	40-41	0.27941958011873425
1106	42-43	0.1844249248070824
1106	44-45	0.034662182591347346
1106	46-47	0.19740151610695023
1106	48-49	0.22091129575230184
1106	50-51	0.0036245538998329607
1106	52-53	0.11055168633847501
1106	54-55	0.1219850221283778
1106	56-57	-0.08397917329315163
1106	58-59	0.07324768532709669
1106	60-61	0.18703803753808756
1106	62-63	0.33962070678171585
1106	64-65	0.1660392098524781
1106	66-67	0.21515867209259198
1106	68-69	0.3139174946351808
1106	70-71	0.33992587730196533
1106	72-73	0.340819325679238
1106	74-75	0.24379698402590222
1106	76-77	0.3268712211808733
1106	78-79	0.22840926058352196
1106	80-81	0.3481031154243972
1106	82-83	0.10160178892154192
1106	84-85	0.10911942686079001
1106	86-87	-0.006271754811326247
1106	88-89	-0.06010719630630135
1106	90-91	0.0052891711688971554
1106	92-93	-0.02399822296618126
1106	94-95	-0.15549659471880517
1106	96-97	-0.20099093862851092
1106	98-99	-0.1375833861305722
1106	100	-0.13360634690186401
1107	1	-0.06223657042203712
1107	2	-0.014066500652045733
1107	3	-0.12743684242781228
1107	4	0.10379536977182369
1107	5	-0.022736366944869246
1107	6	0.03426945642745238
1107	7	2.222445521979921E-4
1107	8	0.10936277954762375
1107	9	0.06637044547763082
1107	10-11	-0.012973546220180765
1107	12-13	0.024211458042422862
1107	14-15	0.008812116441546891
1107	16-17	-0.10813388477817654
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2316	98-99	0.43529752822245626
2316	100	0.12255209123231126
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	3105.0
1	283.0
2	183.0
3	150.0
4	108.0
5	98.0
6	132.0
7	494.0
8	1513.0
9	3188.0
10	5298.0
11	6719.0
12	6656.0
13	5932.0
14	5438.0
15	6488.0
16	9138.0
17	13950.0
18	17642.0
19	22743.0
20	29017.0
21	34091.0
22	31667.0
23	27898.0
24	25977.0
25	25443.0
26	27063.0
27	29985.0
28	35574.0
29	41919.0
30	50933.0
31	62927.0
32	79948.0
33	104598.0
34	146359.0
35	232647.0
36	253836.0
37	255607.0
38	179452.0
39	16789.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.14707262661783	17.022037317532657	12.811693034490581	19.019197021358938
2	13.611357596373269	25.17259370344832	34.09990404206813	27.11614465811028
3	26.769708333867033	29.696783725549924	28.43061199599699	15.102895944586054
4	13.474349078847862	18.984833651317658	34.071101517748644	33.46971575208583
5	9.837162030462506	41.55525854147523	33.724612257399826	14.882967170662434
6	28.083211660141867	31.611335284186453	17.693165352893075	22.612287702778602
7	20.670893918468707	36.89009200980384	22.46989739145053	19.969116680276926
8	28.076169673408245	26.537924800009797	22.202405504594307	23.183500021987648
9	27.89394867227078	17.90897956911429	21.730557256582976	32.466514502031956
10-11	24.28827813944764	26.972087150671946	23.44533519157402	25.29429951830639
12-13	31.462766557018064	25.36069510850703	23.840180039315413	19.336358295159496
14-15	28.756763073652635	24.371995831901536	27.989977643076656	18.881263451369172
16-17	19.820874972277807	29.035746256425497	31.51141820484844	19.631960566448257
18-19	21.234257571895014	29.100990883780163	32.364862533104606	17.29988901122022
20-21	22.310945909164534	25.248122970807458	30.298610310983758	22.14232080904425
22-23	18.94989697997148	27.792588568839754	28.235864104838882	25.021650346349883
24-25	22.826228820245184	28.674174550649834	25.63727453181009	22.862322097294896
26-27	23.06998735193485	23.274053934466604	30.694669584951445	22.961289128647103
28-29	20.756168415685565	27.09215705156215	31.482896760478944	20.66877777227334
30-31	23.65449057838692	26.054682477975792	29.316112831170106	20.974714112467186
32-33	18.49064207437358	27.637162742854088	32.57002858809542	21.302166594676912
34-35	17.88206956772421	27.64668954758885	32.39717080940474	22.074070075282204
36-37	21.616783131155433	26.06736851479252	31.70928682545911	20.606561528592938
38-39	23.253955885545086	25.056047509587074	30.157289237431055	21.53270736743679
40-41	19.392606604045614	28.202491185216772	29.780635198790478	22.624267011947136
42-43	21.79684949657234	27.079423822387817	28.411915952808403	22.71181072823144
44-45	23.64149882851848	23.854090724426364	29.164435935821736	23.33997451123342
46-47	21.146065946236245	27.76843268121719	32.3046677713823	18.780833601164264
48-49	21.63085781234129	26.729643497704807	32.07774841827413	19.561750271679767
50-51	17.02664535149853	27.910403944697435	33.21993997804482	21.843010725759214
52-53	17.95387953395274	29.938087095531674	26.614394430232068	25.49363894028352
54-55	18.041127929900874	27.881525553887688	26.888467607322653	27.188878908888785
56-57	21.511267049759162	24.267451110060883	27.37502595421037	26.846255885969583
58-59	23.27177640221772	23.326440070361382	29.454198303310992	23.947585224109908
60-61	21.990320183055562	26.07796000904926	30.00882386578819	21.922895942106983
62-63	17.550000765442096	26.449308944956364	31.010787166736947	24.98990312286459
64-65	15.970682900381089	29.26610948627998	30.379431159424325	24.383776453914606
66-67	20.9062897510765	25.82171476923848	26.955022309313133	26.316973170371895
68-69	21.147270569267906	24.528078152974604	27.642055323469943	26.682595954287546
70-71	20.919480707498447	25.81344781600363	27.2766655251809	25.990405951317026
72-73	21.890229624062957	23.217760705269335	30.40374174539236	24.488267925275352
74-75	21.100760618750535	25.72237624390799	28.083209013289046	25.093654124052428
76-77	19.254408540070134	24.203119515161504	30.9288590088381	25.61361293593027
78-79	20.677939680237618	24.470131710256922	31.40455216703249	23.447376442472972
80-81	20.410525630228012	24.34619775746986	30.252391796501215	24.990884815800914
82-83	18.738425626307055	24.242462200005736	29.811633983544173	27.207478190143036
84-85	19.380820398495814	25.916119448524377	28.070425499456032	26.63263465352378
86-87	20.550254151889856	24.009141673672314	28.18566728093666	27.25493689350117
88-89	20.376491995271717	22.785176622952882	29.065295620659455	27.773035761115946
90-91	22.157720079692652	22.429022512311747	28.683648725426963	26.72960868256864
92-93	21.342006812092997	23.87433196902218	29.279280783696844	25.504380435187983
94-95	19.86366807320847	23.43011172347674	30.08321776084261	26.62300244247218
96-97	20.776900110655653	23.040093535343694	29.927496189687453	26.255510164313208
98-99	23.01512172895785	22.669622968528245	27.215326751423756	27.099928551090148
100	20.979530657799568	20.56878524145004	29.102958068555562	29.348726032194822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5507.0
1	4107.5
2	2592.0
3	2106.5
4	1439.0
5	1313.0
6	1831.0
7	2935.0
8	4333.5
9	4946.0
10	4060.0
11	3098.5
12	3004.0
13	3116.0
14	3248.0
15	3337.0
16	3422.0
17	3516.0
18	3749.5
19	4082.0
20	4532.5
21	5084.0
22	5855.0
23	6799.0
24	8144.0
25	9892.5
26	11659.5
27	13419.5
28	15546.0
29	17834.0
30	19363.0
31	20422.5
32	21481.0
33	21686.5
34	21894.5
35	22359.5
36	22334.5
37	20822.5
38	18731.0
39	17761.0
40	16637.5
41	15497.0
42	14845.0
43	14944.0
44	16077.5
45	19015.0
46	35369.0
47	85755.5
48	175859.5
49	230547.0
50	282469.0
51	244095.5
52	104770.5
53	42635.5
54	24870.0
55	16639.0
56	12725.0
57	10899.0
58	9818.5
59	8979.5
60	8039.5
61	6871.0
62	5835.5
63	4865.0
64	3797.5
65	3313.0
66	2807.0
67	2209.5
68	1854.5
69	1726.0
70	1566.5
71	1318.5
72	1145.0
73	923.0
74	754.5
75	620.0
76	489.5
77	394.5
78	325.0
79	270.5
80	200.0
81	146.5
82	124.0
83	86.0
84	64.0
85	50.5
86	35.0
87	27.0
88	23.5
89	15.5
90	7.0
91	4.5
92	6.0
93	3.5
94	2.0
95	2.0
96	3.0
97	2.5
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14369858389154422
2	0.06912856914411614
3	0.29666983529077307
4	0.29461541195074714
5	0.29661431033563723
6	0.25058412252802903
7	0.25280512073346245
8	0.25147252181020235
9	0.2605230894973437
10-11	0.23934031911302217
12-13	0.24961243581315185
14-15	0.22262730761713542
16-17	0.230234226470745
18-19	0.24508715196958122
20-21	0.24447637746308704
22-23	0.2362031591478474
24-25	0.2364530214459587
26-27	0.23734142072813205
28-29	0.2476968248609655
30-31	0.23425978571809314
32-33	0.24653080080311293
34-35	0.23059513867912795
36-37	0.2391459817700467
38-39	0.23886835699436756
40-41	0.2541932446118584
42-43	0.2420055169595423
44-45	0.22887386506991703
46-47	0.2304008013361525
48-49	0.23872954460652795
50-51	0.2304008013361525
52-53	0.2463087009825696
54-55	0.2566085801602672
56-57	0.25313827046427745
58-59	0.2527495957783266
60-61	0.23101157584264673
62-63	0.2578856541283915
64-65	0.25352694515022833
66-67	0.25857971606758945
68-69	0.2620777882411471
70-71	0.282455446775999
72-73	0.29164482685097987
74-75	0.2714337431815355
76-77	0.26915722002096626
78-79	0.2673248965014836
80-81	0.25202777136156074
82-83	0.2625497503598017
84-85	0.2741822284607594
86-87	0.2677413336650024
88-89	0.2771250510829587
90-91	0.2688240702901512
92-93	0.24880732396368224
94-95	0.2699068069153
96-97	0.270934018585313
98-99	0.2556091309678222
100	0.2770140011726871
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1800992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.21668073171379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87608721640048	14.965199046726656
2	8.556554119727052	3.2885713736485016
3	3.394833412634204	1.9571228948383763
4	1.9298379688155336	1.4834032044266856
5	1.28405983320562	1.233768392756503
6	0.9500394721425649	1.095396313121374
7	0.743072684196386	0.9995573372861676
8	0.5718718273319428	0.879158266023976
9	0.47338407257026677	0.8187183527455124
>10	3.6093716908755997	13.912230047378019
>50	0.36042399159172106	4.742260781496528
>100	0.20217453984176736	7.295926684790737
>500	0.01949020743784769	2.6367411398450673
>1k	0.014254032305291594	5.824890323351764
>5k	0.007563364080358805	10.331633681016056
>10k+	0.006981566843408127	28.535422160548094
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	63439	3.5224476288623157	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	56730	3.1499307048559904	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	45371	2.5192227394680264	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	32153	1.7852938824825428	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	27113	1.5054481085979283	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	23977	1.3313218492919456	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21271	1.1810713206943728	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	20172	1.1200493950000887	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	19081	1.0594716689468915	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18629	1.0343743892254935	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	18437	1.023713597839413	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17515	0.9725195892041719	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16433	0.9124415877471971	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	15613	0.8669111245358114	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14186	0.7876770135569731	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	12553	0.6970047618201525	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12197	0.6772378777917948	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11506	0.6388701337929319	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	10944	0.6076651090065919	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	10923	0.6064990849487394	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10855	0.6027233879995024	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	10724	0.5954496188767079	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	10442	0.5797915815284022	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	10199	0.5662990174303939	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	9555	0.5305409463229154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9002	0.4998356461327979	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	8834	0.49050745366997744	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	8206	0.4556377818446723	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	8102	0.4498631865105453	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	8022	0.4454211900996784	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	7886	0.43786979620120464	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7680	0.42643165544322237	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7620	0.4231001581350723	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	7588	0.4213233595707255	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7429	0.4124948917041275	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	7380	0.40977416890247154	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	7316	0.406220571773778	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7242	0.40211172509372606	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	7095	0.39394955668875825	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	6809	0.378069419519909	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	6567	0.36463238037703666	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6305	0.3500848421314475	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	6285	0.3489743430287308	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	6213	0.34497654625895063	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	6200	0.34425472184218475	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5995	0.3328721060393383	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5532	0.3071640518114461	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	5339	0.29644773547022973	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	5323	0.29555933618805635	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5295	0.2940046374442529	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4719	0.26202226328601125	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4328	0.2403120058278993	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4312	0.23942360654572592	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4178	0.23198326255752388	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	3967	0.22026749702386242	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	3909	0.2170470496259839	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	3763	0.2089404061761518	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	3732	0.20721913256694086	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3579	0.19872381443115794	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2975	0.16518674152911286	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT	2788	0.15480357491871147	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	2744	0.1523604768927347	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	2696	0.14969527904621455	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	2688	0.14925107940512783	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT	2638	0.14647483164833602	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2557	0.1419773102823333	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	2507	0.13920106252554149	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	2129	0.11821262948419538	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	2103	0.11676898065066364	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	2086	0.11582505641335442	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	1916	0.10638581404026225	No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC	1907	0.10588608944403972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	5.552495513583625E-5	0.0
7	0.0	0.0	0.0	1.110499102716725E-4	0.0
8	0.0	0.0	0.0	1.6657486540750874E-4	0.0
9	0.0	0.0	0.0	3.331497308150175E-4	0.0
10-11	0.0	0.0	0.0	4.4419964108668997E-4	0.0
12-13	0.0	0.0	0.0	6.66299461630035E-4	0.0
14-15	0.0	0.0	0.0	7.218244167658713E-4	0.0
16-17	0.0	0.0	0.0	8.328743270375438E-4	0.0
18-19	0.0	0.0	0.0	0.0012493114905563155	0.0
20-21	0.0	0.0	0.0	0.005219345782768607	0.0
22-23	0.0	0.0	0.0	0.01823994776212221	0.0
24-25	0.0	0.0	0.0	0.03850655638670244	0.0
26-27	0.0	0.0	0.0	0.07301531600362467	0.0
28-29	0.0	0.0	0.0	0.20552562143529787	0.0
30-31	0.0	0.0	0.0	0.4783197260176614	0.0
32-33	0.0	0.0	0.0	0.9512535313871466	0.0
34-35	0.0	0.0	0.0	1.6986749524706384	0.0
36-37	0.0	0.0	0.0	2.5863524102272524	0.0
38-39	0.0	0.0	0.0	3.7151192231836676	0.0
40-41	0.0	0.0	0.0	5.280950720491818	0.0
42-43	0.0	0.0	0.0	6.441727670084043	0.0
44-45	0.0	0.0	0.0	7.44431402249427	0.0
46-47	0.0	0.0	0.0	8.629827339599508	0.0
48-49	0.0	0.0	0.0	9.720837183063555	0.0
50-51	0.0	0.0	0.0	10.832779934613813	0.0
52-53	0.0	0.0	0.0	11.975011549190668	0.0
54-55	0.0	0.0	0.0	13.054388914553758	0.0
56-57	0.0	0.0	0.0	14.215387963966524	0.0
58-59	0.0	0.0	0.0	15.247846742239833	0.0
60-61	0.0	0.0	0.0	16.198350686732645	0.0
62-63	0.0	0.0	0.0	17.10618370320357	0.0
64-65	0.0	0.0	0.0	18.094194754890637	5.552495513583625E-5
66-67	0.0	0.0	0.0	19.112272569783762	5.552495513583625E-5
68-69	0.0	0.0	0.0	20.342872150459304	5.552495513583625E-5
70-71	0.0	0.0	0.0	21.95942569428404	5.552495513583625E-5
72-73	0.0	0.0	0.0	23.182224018762994	5.552495513583625E-5
74-75	0.0	0.0	0.0	24.273317149659743	5.552495513583625E-5
76-77	0.0	0.0	0.0	25.358247010536417	5.552495513583625E-5
78-79	0.0	0.0	0.0	26.588957641122228	5.552495513583625E-5
80-81	0.0	0.0	0.0	27.81680873651854	5.552495513583625E-5
82-83	0.0	0.0	0.0	29.219896590323554	5.552495513583625E-5
84-85	0.0	0.0	0.0	30.54949716600629	5.552495513583625E-5
86-87	0.0	0.0	0.0	31.66685360068229	5.552495513583625E-5
88	0.0	0.0	0.0	32.65217169204527	1.110499102716725E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCA	1065	0.0	41.965473	1
CGTGTCA	60	8.823556E-5	39.16856	94
TATCCGC	50	0.0016942099	37.636402	1
TACCCAT	1170	0.0	36.577694	2
ACCCATG	1170	0.0	36.577694	3
ACAGCGT	65	1.4147938E-4	36.149555	8
TAATGCG	55	0.0027104695	34.19966	5
CCGTTAC	60	0.004164587	31.328741	9
GCGTTAT	65	0.006126044	28.951082	1
ACGAGAG	65	0.0061368966	28.940596	3
TCCGTTA	70	0.008842444	26.853952	8
GCGTATC	90	9.4661483E-4	26.13639	1
ATTAGTG	110	1.0150426E-4	25.649744	5
GCCGTAT	1365	0.0	24.792406	94
TACTAAG	115	1.3719802E-4	24.536589	2
ATGGGCG	230	1.5279511E-10	24.534538	5
GCCGCAT	320	0.0	23.501135	94
CCGTATC	885	0.0	22.837261	94
ATGGGGG	890	0.0	22.719717	5
GTATAGG	150	3.2682015E-5	21.954567	1
>>END_MODULE