##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522930_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800992
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01313276238873	33.0	31.0	34.0	30.0	34.0
2	32.05749498054406	33.0	31.0	34.0	30.0	34.0
3	32.24235032693094	34.0	31.0	34.0	30.0	35.0
4	35.326546703150264	37.0	35.0	37.0	32.0	37.0
5	35.28286355519625	36.0	35.0	37.0	32.0	37.0
6	35.52683743181535	36.0	35.0	37.0	33.0	37.0
7	35.33040013503669	36.0	35.0	37.0	32.0	37.0
8	35.40000399779677	36.0	35.0	37.0	33.0	37.0
9	36.551833100868855	37.0	35.0	39.0	33.0	39.0
10-11	36.364449703274644	37.0	35.0	39.0	32.0	39.0
12-13	36.419889427604346	37.0	35.0	39.0	32.5	39.0
14-15	37.294434955846555	38.0	37.0	40.0	32.0	41.0
16-17	37.05930981370267	38.0	36.0	40.0	32.0	41.0
18-19	37.110996051065186	38.0	36.0	40.0	32.0	41.0
20-21	37.23680421678719	38.0	36.0	40.0	32.5	41.0
22-23	37.173048519926795	38.0	35.5	40.0	32.0	41.0
24-25	37.075207163607615	38.0	36.5	40.0	32.0	41.0
26-27	36.626478629555265	37.5	35.5	40.0	31.5	41.0
28-29	36.61811240694018	38.0	35.5	40.0	31.0	41.0
30-31	36.309276776354366	37.0	35.0	40.0	31.0	41.0
32-33	36.00771741351433	37.0	35.0	40.0	30.0	41.0
34-35	35.81643088919884	37.0	35.0	40.0	30.0	41.0
36-37	35.63115133215472	37.0	35.0	40.0	30.0	41.0
38-39	35.48367510794051	36.5	34.5	40.0	29.0	41.0
40-41	35.62017738002167	37.0	35.0	40.0	29.5	41.0
42-43	35.54714318553331	37.5	35.0	40.0	28.5	41.0
44-45	35.18031729180363	37.0	35.0	40.0	27.0	41.0
46-47	34.80272510927311	37.0	34.5	40.0	25.5	41.0
48-49	34.50060244576322	37.0	34.0	40.0	24.0	41.0
50-51	34.23420703701071	37.0	34.0	40.0	23.0	41.0
52-53	34.32388344867717	37.5	34.0	40.0	22.0	41.0
54-55	34.2042174534923	37.5	34.0	40.0	21.5	41.0
56-57	33.82610472450738	37.0	33.5	40.0	19.5	41.0
58-59	33.635671063502784	37.0	33.0	40.0	18.5	41.0
60-61	33.22303985803379	36.5	32.5	39.0	17.5	41.0
62-63	32.86378034994048	36.0	32.0	39.0	14.0	40.5
64-65	32.715552317833726	36.5	31.5	39.0	13.0	40.0
66-67	32.051113775075066	35.5	31.0	37.0	11.5	40.0
68-69	31.801103225333595	35.5	31.0	37.0	11.5	39.0
70-71	31.175882236012153	35.0	30.0	37.0	11.0	39.0
72-73	30.82650200556138	35.0	30.0	36.0	11.0	38.5
74-75	30.58134600264743	35.0	29.0	36.5	10.0	38.0
76-77	28.995489985519093	33.0	26.5	35.5	10.0	36.0
78-79	29.929500519713578	34.0	29.0	36.0	10.0	37.0
80-81	30.01927576580018	34.0	29.0	36.0	10.0	37.5
82-83	29.928351430767044	34.0	29.0	36.0	10.0	37.0
84-85	29.61989781187257	34.0	29.0	35.5	10.0	36.0
86-87	29.545227852205898	34.0	29.0	35.0	10.0	36.0
88-89	29.4251956699419	34.0	28.0	35.0	10.0	37.0
90-91	29.141818508910646	34.0	27.5	35.0	9.0	36.5
92-93	28.858549899166682	34.0	26.0	35.0	8.0	36.5
94-95	28.991997465841045	34.0	26.5	35.0	8.0	36.5
96-97	28.76090954318509	34.0	25.5	35.0	8.0	37.0
98-99	28.61657325518381	34.0	25.0	35.0	8.0	36.0
100	27.37399055631563	33.0	23.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04785140770523455
1101	2	-0.029454961377346933
1101	3	-0.05324919352045754
1101	4	-0.04397779297999449
1101	5	-0.18193258648034316
1101	6	-0.05697504140757559
1101	7	-0.04360655082986398
1101	8	-0.14503802508411923
1101	9	-0.07767585988747072
1101	10-11	-0.17532278133374746
1101	12-13	-0.03843543227753088
1101	14-15	-0.032579948455200736
1101	16-17	-0.2171344563644908
1101	18-19	-0.40503292438524596
1101	20-21	-0.2663788159591789
1101	22-23	-0.7198855914863813
1101	24-25	-0.2994049432058645
1101	26-27	-0.24143908816490267
1101	28-29	-0.25673379412647535
1101	30-31	-0.2855364419632096
1101	32-33	-0.21937219278446918
1101	34-35	-0.2823346626174157
1101	36-37	-0.25972125779347977
1101	38-39	-0.24737844179149704
1101	40-41	-0.43866991454993354
1101	42-43	-0.43525371588929573
1101	44-45	-0.4916621880023726
1101	46-47	-0.5994383570225779
1101	48-49	-0.4799748093350047
1101	50-51	-0.4061509319365868
1101	52-53	-0.3832997073957145
1101	54-55	-0.3969522203281173
1101	56-57	-0.43901790373130467
1101	58-59	-0.5094752892060797
1101	60-61	-0.6080877723286093
1101	62-63	-0.32755056342578115
1101	64-65	-0.5013601535927918
1101	66-67	-0.3779368253484279
1101	68-69	-0.3675457207413366
1101	70-71	-0.4171673978085799
1101	72-73	-0.3871861908765517
1101	74-75	-0.3927396518353028
1101	76-77	-0.6093964845236641
1101	78-79	-0.5573921727678091
1101	80-81	-0.5160775246476668
1101	82-83	-0.523732419574543
1101	84-85	-0.4479975567835659
1101	86-87	-0.5036186832655467
1101	88-89	-0.6315358183400157
1101	90-91	-0.7463005277253423
1101	92-93	-0.717112902874522
1101	94-95	-0.7255245882293622
1101	96-97	-0.8436207253460424
1101	98-99	-0.9264725662918316
1101	100	-0.9866473682632986
1102	1	0.08902865831554152
1102	2	-0.03449625262620515
1102	3	-0.010196495599437583
1102	4	-0.09796058970608357
1102	5	0.004050786797066053
1102	6	-0.10515629048904884
1102	7	-0.05752362353185703
1102	8	-0.20802555009627355
1102	9	-0.052686670224694865
1102	10-11	-0.1033499583802211
1102	12-13	-0.003889515900077356
1102	14-15	-0.10620929661305922
1102	16-17	-0.1565432227707717
1102	18-19	-0.0983434806442105
1102	20-21	-0.0798950229863209
1102	22-23	-0.09911045497937465
1102	24-25	-0.12427642030773711
1102	26-27	0.004148810475115283
1102	28-29	-0.031515609019578505
1102	30-31	-0.16751829472783442
1102	32-33	-0.0711820764002411
1102	34-35	-0.026307546334933818
1102	36-37	-0.07516407181611129
1102	38-39	-0.07868719208825325
1102	40-41	0.1120574162435588
1102	42-43	0.044304501662736584
1102	44-45	0.22035555084248415
1102	46-47	0.12734291099464912
1102	48-49	0.08872965300263047
1102	50-51	0.26211885592643824
1102	52-53	0.10405555420032186
1102	54-55	0.10972369282742278
1102	56-57	0.005036128171205689
1102	58-59	0.04591532431235379
1102	60-61	-0.12007541180316395
1102	62-63	0.02944033328109441
1102	64-65	0.13080964359680536
1102	66-67	0.08770449614110731
1102	68-69	0.030452211869988588
1102	70-71	0.15812978048143833
1102	72-73	0.1390403584638058
1102	74-75	-0.007259277189994862
1102	76-77	-0.04902163431395934
1102	78-79	0.011138123025627777
1102	80-81	0.0012725366815686812
1102	82-83	0.004435574479064286
1102	84-85	-0.0877088006647746
1102	86-87	-0.10657725353132719
1102	88-89	-0.27871326048704503
1102	90-91	-0.4276757298009244
1102	92-93	-0.2957392057674504
1102	94-95	-0.49415298959952025
1102	96-97	-0.5449256319673381
1102	98-99	-0.5008987352213694
1102	100	-0.574215339141805
1103	1	6.986510586486361E-4
1103	2	-0.0906918259208318
1103	3	-0.2702427582990019
1103	4	-0.06356971525035249
1103	5	-0.14859344397216745
1103	6	-0.14209841675900492
1103	7	-0.032544033546372475
1103	8	0.007164036260768114
1103	9	0.010867847191846636
1103	10-11	-0.20482174720460478
1103	12-13	-0.1163033402832454
1103	14-15	-0.1730407552487634
1103	16-17	-0.300842025383254
1103	18-19	-0.29806681155857717
1103	20-21	-0.24843638728240336
1103	22-23	-0.3961158522943222
1103	24-25	-0.29196169788392723
1103	26-27	-0.2643109137629125
1103	28-29	-0.19762477991943683
1103	30-31	-0.4062360922604924
1103	32-33	-0.23709281660342896
1103	34-35	-0.30773329923768955
1103	36-37	-0.4562700678248177
1103	38-39	-0.2195673172696786
1103	40-41	-0.4161821066156577
1103	42-43	-0.2113989635041733
1103	44-45	-0.08546583885271986
1103	46-47	-0.15202483211130158
1103	48-49	0.04261435909841538
1103	50-51	-0.18854560343495308
1103	52-53	-0.12090816132943161
1103	54-55	-0.8653930978111788
1103	56-57	-0.00732893895506237
1103	58-59	0.023917864225268204
1103	60-61	0.0037056567744926383
1103	62-63	0.12238527159748713
1103	64-65	0.14051573938780848
1103	66-67	0.22265437821658196
1103	68-69	0.39039982616607105
1103	70-71	0.3002912010184495
1103	72-73	0.10032936426931727
1103	74-75	-0.08536726884456414
1103	76-77	-0.03129326892934259
1103	78-79	0.03690009690080842
1103	80-81	-0.7627083232603802
1103	82-83	0.03520934080707505
1103	84-85	0.17244518155583322
1103	86-87	0.10683779545270866
1103	88-89	-0.10238774343334711
1103	90-91	-0.19373727733358237
1103	92-93	-0.3155425892943029
1103	94-95	-0.2169226285690371
1103	96-97	-0.14253085693105305
1103	98-99	-0.12986167970612783
1103	100	-0.251703093133095
1104	1	0.02837697893644986
1104	2	-0.06755693775934901
1104	3	-0.138500055080236
1104	4	-0.035369985754080346
1104	5	-0.10166729830931587
1104	6	-0.24481879773736637
1104	7	-0.19459764556673775
1104	8	-0.05864054184434764
1104	9	-0.07398138675977606
1104	10-11	-0.1417548827747055
1104	12-13	-0.11046136901574499
1104	14-15	-0.2112778210789159
1104	16-17	-0.24838078242999018
1104	18-19	-0.2366295047755287
1104	20-21	-0.37325737746244414
1104	22-23	-0.12169592935378404
1104	24-25	-0.10931840478518495
1104	26-27	-0.2972487206368868
1104	28-29	-0.387078970454084
1104	30-31	-0.35806398142215556
1104	32-33	-0.13660579623608982
1104	34-35	-0.40206792257829704
1104	36-37	-0.4355113219164721
1104	38-39	-0.34216418147225625
1104	40-41	-0.20476369531360206
1104	42-43	-0.1635479337714969
1104	44-45	-0.3029443008458088
1104	46-47	-0.1485412441862266
1104	48-49	-0.39097157698086704
1104	50-51	-0.3052869995978966
1104	52-53	-0.29479489073478504
1104	54-55	-0.3531969882912662
1104	56-57	-0.21320230797670803
1104	58-59	-0.2778585951309793
1104	60-61	-0.2569717107014142
1104	62-63	-0.3449198634270587
1104	64-65	-0.6665344060931915
1104	66-67	-0.2500203410505577
1104	68-69	-0.13806486627801107
1104	70-71	-0.017471829759873714
1104	72-73	-0.21770573302109852
1104	74-75	-0.25766351020663336
1104	76-77	-0.1965910787584022
1104	78-79	-0.32497535772730757
1104	80-81	-0.29118935390231115
1104	82-83	-0.1910278159486971
1104	84-85	-0.8615418217629518
1104	86-87	-0.46010690472004967
1104	88-89	-0.28573855153481986
1104	90-91	-0.2930145607032877
1104	92-93	-0.27283382974711046
1104	94-95	-0.062429340016578294
1104	96-97	0.00760723635863414
1104	98-99	-0.12498224139916303
1104	100	-1.5339035500343527
1105	1	0.06297607271107353
1105	2	-0.11637736723137593
1105	3	-0.040452872259116646
1105	4	-0.05005920955234444
1105	5	-0.07986125023997204
1105	6	-0.03254535408093773
1105	7	-0.04049008055491754
1105	8	-0.15598589251416684
1105	9	-0.13935027958877555
1105	10-11	-0.09504610617896248
1105	12-13	-0.05188916649407105
1105	14-15	-0.0835197438527544
1105	16-17	-0.1535841906254234
1105	18-19	-0.18350573606000609
1105	20-21	-0.21476028329379915
1105	22-23	-0.1169247020094204
1105	24-25	-0.11818365301213873
1105	26-27	-0.1681793254438233
1105	28-29	-0.21964890741231358
1105	30-31	-0.25535023043712357
1105	32-33	-0.22941386361197402
1105	34-35	-0.3860920065026505
1105	36-37	-0.35545320528920143
1105	38-39	-0.20633503450613944
1105	40-41	-0.1851123950771978
1105	42-43	-0.29945095780616526
1105	44-45	-0.4507169072287951
1105	46-47	-0.3672358363848005
1105	48-49	-0.5500227491636949
1105	50-51	-0.4812806461699921
1105	52-53	-0.5629932752895499
1105	54-55	-1.4793420828539112
1105	56-57	-0.5594456590405343
1105	58-59	-0.46415193634452834
1105	60-61	-0.36456134821126085
1105	62-63	-0.35491173694951783
1105	64-65	-0.4838610412389741
1105	66-67	-0.3874440014130478
1105	68-69	-0.17119873152623555
1105	70-71	-0.06296779114679296
1105	72-73	-0.058636091571134585
1105	74-75	-0.1114423222633718
1105	76-77	-0.15723922690655456
1105	78-79	-0.057940537002327375
1105	80-81	-0.17681533892989165
1105	82-83	-0.08364749679975958
1105	84-85	-0.06016032058091625
1105	86-87	-0.177828782182619
1105	88-89	-0.2783693139461647
1105	90-91	-0.1457159988435528
1105	92-93	0.0028446079991475415
1105	94-95	-0.05191417059182868
1105	96-97	0.043566899479206
1105	98-99	0.08914428539358354
1105	100	0.31544879433129225
1106	1	0.04841520551805445
1106	2	-0.1463024667242827
1106	3	-0.10954126849764378
1106	4	-0.07017543171038199
1106	5	-0.08193635936122234
1106	6	-0.09464016119307672
1106	7	-0.02480791225143264
1106	8	-0.1731584485190396
1106	9	-0.025668600726206137
1106	10-11	-0.13651870149958967
1106	12-13	-0.052534065075498404
1106	14-15	-0.09398254634328396
1106	16-17	-0.07629765907653763
1106	18-19	-0.1250190512599545
1106	20-21	-0.04062312349535091
1106	22-23	-0.023429953469722875
1106	24-25	-0.053408956305034394
1106	26-27	-0.0678815487893516
1106	28-29	-0.11261580194982912
1106	30-31	-0.19681991719494363
1106	32-33	-0.10253784060023463
1106	34-35	-0.05872674316738369
1106	36-37	0.10100059469795752
1106	38-39	-0.04538168785359886
1106	40-41	0.03538876032430238
1106	42-43	-0.0578010404494691
1106	44-45	0.10955503336431605
1106	46-47	-0.03407250353289015
1106	48-49	0.06012001112259924
1106	50-51	0.22511347813194504
1106	52-53	0.14542230016459
1106	54-55	0.05060508159822774
1106	56-57	0.2315786164264395
1106	58-59	0.02199728696890446
1106	60-61	0.07850905104414352
1106	62-63	-0.017554055936614077
1106	64-65	0.4351445111071328
1106	66-67	0.056518022786193
1106	68-69	0.17922190683309225
1106	70-71	0.22826660290930434
1106	72-73	0.11622168973703495
1106	74-75	0.13036907047685986
1106	76-77	0.17867370075247635
1106	78-79	0.13666387839660743
1106	80-81	0.3245817741710759
1106	82-83	0.2109407710608231
1106	84-85	0.2765158540772781
1106	86-87	0.33738952050048
1106	88-89	0.14279709997641987
1106	90-91	0.1263920873622375
1106	92-93	0.24571712468827656
1106	94-95	0.1356600479074288
1106	96-97	0.2946184953775308
1106	98-99	0.21439953685388602
1106	100	0.13574073380108942
1107	1	-0.012647307441088884
1107	2	-0.03966842776142698
1107	3	0.029139187351283624
1107	4	0.007478046947944961
1107	5	-0.1056416511261844
1107	6	-0.10522949420300165
1107	7	-0.18695045176134073
1107	8	0.019158848728210387
1107	9	-0.19344144044489298
1107	10-11	-0.05248003206308738
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2312	64-65	0.07775082573003544
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2312	92-93	0.20227653354714192
2312	94-95	0.3193962980400684
2312	96-97	-1.1933697501806613
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2313	1	0.16846952347891175
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2313	24-25	-1.2924722708983154
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2313	98-99	0.41274551619756394
2313	100	0.5034710391370716
2314	1	-2.119195519708178
2314	2	0.07185842608924986
2314	3	-6.613500426695396E-4
2314	4	-0.19638096520034765
2314	5	-0.11183259853834926
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2314	10-11	-0.9740315780978364
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2314	84-85	0.1897719683773076
2314	86-87	0.25081777340182754
2314	88-89	0.2965339946861789
2314	90-91	0.33828884746140986
2314	92-93	0.2372706169839489
2314	94-95	0.0827260761291484
2314	96-97	0.1314869787823767
2314	98-99	0.22439889696693882
2314	100	0.3467267815618911
2315	1	0.06490070320113972
2315	2	-0.07254198079951024
2315	3	-8.26457318105156E-4
2315	4	-0.059459783655100296
2315	5	-0.14864991015698337
2315	6	-0.11903601355817983
2315	7	-0.10115448140388139
2315	8	-0.007589771173414306
2315	9	0.0693305149770822
2315	10-11	-0.023528994236102108
2315	12-13	0.05561199270591288
2315	14-15	0.06176984103414895
2315	16-17	0.1993919059538669
2315	18-19	0.016933833769243734
2315	20-21	0.014575035020122584
2315	22-23	0.0595400427753745
2315	24-25	0.20510407715479317
2315	26-27	0.05977272072340867
2315	28-29	-1.534770252944739
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2315	32-33	-1.6249429069493289
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2315	82-83	0.09710434596970074
2315	84-85	0.027926031452583544
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2315	90-91	0.37045872486053355
2315	92-93	0.4745665517743589
2315	94-95	0.27064876082177136
2315	96-97	0.35989718901629075
2315	98-99	0.24371658242603544
2315	100	0.1045380336092876
2316	1	0.03792684361772558
2316	2	-0.19965047348242138
2316	3	-0.09802210721805693
2316	4	-0.11664679544140455
2316	5	-0.09620373808981242
2316	6	-0.07291321062526634
2316	7	-0.07684628970524443
2316	8	0.008853013133567345
2316	9	-0.11063243561358149
2316	10-11	-0.03579444076920879
2316	12-13	-0.015498915949407888
2316	14-15	0.06546634630980463
2316	16-17	-1.5461835914968773
2316	18-19	-0.0948082645500321
2316	20-21	-0.2217819755177004
2316	22-23	-0.06261165734711227
2316	24-25	-0.012917187451549239
2316	26-27	-0.15709623301881948
2316	28-29	-0.21116998165398115
2316	30-31	-0.20935245098346655
2316	32-33	-0.016166785934750294
2316	34-35	0.05871772828869837
2316	36-37	-0.10830744527579839
2316	38-39	-0.20478091392628528
2316	40-41	-0.2653977143331048
2316	42-43	-0.2689066620964269
2316	44-45	-0.04987556107494129
2316	46-47	0.06961606783842456
2316	48-49	-0.011476107216310538
2316	50-51	0.04452927715831123
2316	52-53	-0.1015752679156563
2316	54-55	-0.09299058712741726
2316	56-57	-0.24452217362072304
2316	58-59	-0.36932308600097485
2316	60-61	-1.6586423353069577
2316	62-63	-0.36292848531552835
2316	64-65	-0.15380615603703518
2316	66-67	-0.33824894946064177
2316	68-69	-0.13887249975147498
2316	70-71	-0.1816072875436454
2316	72-73	-0.1943538936492324
2316	74-75	-0.283352672942101
2316	76-77	-0.47474593044266555
2316	78-79	-0.5462054209473557
2316	80-81	-0.2862165246178421
2316	82-83	-0.2417121649747216
2316	84-85	-0.8523061853370741
2316	86-87	-0.20430905912245478
2316	88-89	-0.1371433524077048
2316	90-91	-0.09636383276344773
2316	92-93	0.14908512878010427
2316	94-95	-0.035013927588646965
2316	96-97	0.07178066896135604
2316	98-99	0.09747264344620987
2316	100	-0.06137111098126269
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	22.0
13	187.0
14	733.0
15	2278.0
16	4968.0
17	9561.0
18	14259.0
19	19438.0
20	25368.0
21	30992.0
22	31745.0
23	29927.0
24	28744.0
25	29519.0
26	31418.0
27	35208.0
28	39467.0
29	44952.0
30	52035.0
31	61749.0
32	74251.0
33	94419.0
34	127931.0
35	222312.0
36	331038.0
37	260071.0
38	184071.0
39	14323.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.359486923390136	17.985511601772917	13.433625949131178	20.22137552570577
2	13.114909765755801	25.409967609074346	33.952442591207415	27.522680033962438
3	26.007000586343526	29.239830049217318	29.773313818162432	14.979855546276719
4	13.045317827795918	19.815701466581455	33.23281460040611	33.90616610521652
5	9.522683155430586	41.83424172563443	34.30224810436961	14.340827014565372
6	28.102956592811072	31.909081217462376	17.24643973987669	22.74152244984986
7	19.61413487677902	36.5454149713047	22.172169559886996	21.668280592029284
8	27.755330259840278	27.42095879435266	22.47884636847158	22.34486457733548
9	26.425936372843413	17.649828538938543	21.707037010714096	34.21719807750395
10-11	23.986169844174764	26.69242839501786	23.96304370036069	25.35835806044669
12-13	31.05525294421402	24.865157496710143	24.25404916184987	19.82554039722597
14-15	28.321396293524842	24.1512854935465	27.78637442090664	19.740943792022012
16-17	19.88827659386035	29.09053504911671	31.237550080776117	19.783638276246823
18-19	21.364336987615715	29.01062303441659	31.890258257671327	17.73478172029637
20-21	22.08723699721096	25.205763087742834	29.608340796866596	23.09865911817961
22-23	18.703355230682867	28.315710510783305	27.646296819674188	25.33463743885964
24-25	23.195155579074438	28.95052909156835	25.084332037512002	22.76998329184521
26-27	23.508999909757943	23.66771486286678	30.490458637900275	22.332826589475
28-29	21.06350114686509	26.905085772625565	31.364323818837242	20.667089261672107
30-31	23.14163286033624	26.566069985281715	29.501980068540938	20.7903170858411
32-33	18.597484116081155	28.079775531712865	32.241773179526874	21.080967172679106
34-35	18.404081849104077	27.72970327076012	32.11271563075336	21.753499249382443
36-37	21.300935168966483	26.725459345567742	31.33603142198823	20.637574063477544
38-39	22.897414348614696	24.910247237591683	30.141463846662774	22.050874567130847
40-41	19.154280447056113	27.942395229032275	30.175149266266587	22.72817505764503
42-43	22.036044620432577	26.767039873306313	28.814095783225856	22.38281972303525
44-45	23.119114140998796	23.99888165381966	29.32998903032334	23.5520151748582
46-47	21.119046164611017	27.557101842030736	32.10820056310496	19.215651430253285
48-49	21.57370124650236	26.463996765663612	31.90366853383322	20.058633454000812
50-51	16.669647007339968	27.70117438380531	33.158557640991866	22.470620967862857
52-53	17.48278950749976	29.718773583386408	26.93266459017176	25.865772318942064
54-55	17.575205165571997	27.629435675640856	26.73034140539562	28.065017753391526
56-57	21.355184660352887	24.425240764761277	26.731523969864423	27.488050605021414
58-59	22.202111136469775	23.89782828038833	29.39395756736751	24.506103015774382
60-61	21.52109593178939	27.233604014322427	28.762129352178555	22.483170701709625
62-63	16.691037515189556	28.52877347215324	29.424363981931055	25.355825030726148
64-65	15.039550513384874	31.20967491177065	28.54283315602325	25.20794141882122
66-67	20.377111559879378	27.68608073719905	25.089742856524143	26.847064846397434
68-69	20.95325798226755	26.109333078658874	25.853168698139694	27.084240240933887
70-71	21.10984143694541	27.51599539585794	25.02344547739656	26.350717689800092
72-73	22.345720691956675	25.152144290535627	27.900153365887032	24.60198165162067
74-75	22.12086380471661	27.334316191618495	25.38845113750576	25.156368866159134
76-77	19.97294091646612	26.67254899565433	28.168628121678697	25.185881966200856
78-79	21.461169973230245	27.09014816373695	28.227848874432027	23.220832988600783
80-81	20.988855305535868	26.646405604912626	26.988744810844473	25.375994278707037
82-83	18.661371022506117	26.629185158944285	27.09293344667922	27.61651037187038
84-85	20.004519747660378	27.402552657975317	25.155866052225296	27.437061542139013
86-87	20.98664424188228	25.903180123526955	25.439489034741296	27.67068659984947
88-89	20.473790599646694	24.900144948418518	26.401878304961368	28.224186146973423
90-91	22.199028085788754	24.496302572707556	26.091709967117936	27.212959374385754
92-93	20.431469685460836	26.274803107634494	26.826973476721616	26.466753730183058
94-95	19.051533558042888	25.866890543347925	27.621785000095784	27.459790898513404
96-97	19.58621179819156	25.73756396292947	27.729462107542957	26.946762131336012
98-99	21.995583520546013	25.083843147793814	25.260580061322102	27.659993270338074
100	19.59511157752125	24.322844657660507	27.275998200989456	28.806045563828782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4116.0
1	2622.0
2	1352.0
3	1577.5
4	1314.5
5	1225.5
6	1723.5
7	2750.0
8	3922.0
9	4367.0
10	3572.0
11	2724.5
12	2735.0
13	2853.0
14	2918.0
15	3022.0
16	2999.5
17	3073.5
18	3265.5
19	3414.5
20	3815.5
21	4319.5
22	4826.5
23	5417.0
24	6490.5
25	7917.0
26	9274.5
27	10749.0
28	12525.0
29	14616.0
30	16454.5
31	18065.5
32	19988.5
33	21390.0
34	22671.5
35	24210.5
36	25363.0
37	24760.0
38	22923.5
39	22057.5
40	20750.5
41	19286.5
42	18698.5
43	18804.5
44	19517.5
45	21366.5
46	32653.5
47	80527.0
48	159346.5
49	210641.5
50	291793.0
51	282872.0
52	132417.0
53	40901.5
54	16801.5
55	11771.5
56	9916.0
57	9201.5
58	8417.0
59	7683.5
60	6820.5
61	5597.5
62	4635.0
63	3710.0
64	2725.5
65	2315.5
66	1928.0
67	1372.0
68	1075.0
69	1082.0
70	976.0
71	814.5
72	748.0
73	610.5
74	465.0
75	328.5
76	272.5
77	228.0
78	175.0
79	130.0
80	82.0
81	61.0
82	47.5
83	32.5
84	25.0
85	15.0
86	10.0
87	8.5
88	5.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044031289422718146
2	0.00993896696931469
3	0.0
4	5.552495513583625E-5
5	4.4419964108669E-4
6	0.0
7	0.0
8	5.552495513583625E-5
9	0.0
10-11	0.0
12-13	1.110499102716725E-4
14-15	0.012465352427995239
16-17	0.0014714113110996605
18-19	0.0
20-21	7.218244167658713E-4
22-23	7.218244167658713E-4
24-25	0.0041643716351877184
26-27	0.01551922496046623
28-29	0.01185457792150104
30-31	0.009467004850660082
32-33	0.006329844885485333
34-35	0.00785678115172083
36-37	0.004858433574385672
38-39	0.004025559247348128
40-41	0.005524733036015707
42-43	0.006274319930349497
44-45	0.006579707183596596
46-47	0.014547538245589098
48-49	0.017407073435084665
50-51	0.016824061406158382
52-53	0.011160515982303087
54-55	0.0038034594268047833
56-57	0.00355359712869352
58-59	0.008939517776869636
60-61	0.002248760683001368
62-63	8.328743270375437E-5
64-65	2.22099820543345E-4
66-67	7.218244167658713E-4
68-69	0.0
70-71	2.7762477567918123E-4
72-73	0.0038867468595085376
74-75	0.006774044526572022
76-77	0.006551944706028677
78-79	0.004386471455731064
80-81	2.7762477567918126E-5
82-83	8.328743270375437E-4
84-85	3.6091220838293565E-4
86-87	4.7196211865460814E-4
88-89	3.6091220838293565E-4
90-91	5.552495513583625E-4
92-93	4.4419964108669E-4
94-95	2.7762477567918126E-5
96-97	0.0025819104138163854
98-99	5.552495513583625E-4
100	1.110499102716725E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1800992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.855540806951762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.84924560515546	12.447689728603011
2	10.141208668757411	3.4187111309610705
3	4.059247789350357	2.05262450276771
4	2.291113379526425	1.5447182024784327
5	1.5520979352852757	1.3080725042293273
6	1.123888429636488	1.1366248372919254
7	0.8612857543126721	1.0162206024784433
8	0.6626074405957524	0.8934885403161256
9	0.55094898822862	0.835788883827266
>10	4.187533030786222	14.129528186540075
>50	0.4298364309754496	4.972148996161393
>100	0.23418437889244414	7.487672425139602
>500	0.02258799682933642	2.704995290605795
>1k	0.016940997622002317	6.112778726758947
>5k	0.008968763446942403	10.82093299467894
>10k+	0.008304410599020743	29.118004447161933
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	59992	3.331053108509088	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	58575	3.252374247081608	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	43550	2.4181117961656686	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	31438	1.74559353956042	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	27911	1.5497570227963255	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	25646	1.4239929994136564	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21429	1.189844263605835	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	20462	1.1361516319894813	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	19644	1.0907322186883672	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	19409	1.0776838542314457	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	18747	1.0409263339315222	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17963	0.9973947691050266	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	16396	0.9103871644071713	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	13370	0.7423686501661306	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13223	0.7342064817611628	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12572	0.6980597359677334	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12477	0.6927848652298289	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	12239	0.6795699259074999	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	11650	0.6468657273324924	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11345	0.6299306160160623	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	10939	0.6073874842309128	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	10431	0.579180807021908	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	10316	0.5727954371812868	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10235	0.568297915815284	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	10097	0.5606354720065386	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	9818	0.5451440095236403	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	8333	0.46268945114692345	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	8288	0.4601908281658108	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	8282	0.4598576784349958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8086	0.44897478722837186	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	7915	0.43948001990014396	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7910	0.43920239512446474	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	7849	0.4358153728611787	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	7662	0.4254322062507774	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7561	0.4198241857820579	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	7518	0.41743661271121696	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	7507	0.41682583820472274	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	7459	0.4141606403582026	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7451	0.41371644071711594	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	7361	0.4087191947548906	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7200	0.39977967697802097	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7138	0.39633712975959917	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	6632	0.368241502460866	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	6354	0.35280556493310355	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	6322	0.35102876636875674	TruSeq Adapter, Index 3 (95% over 21bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	6279	0.3486411932979158	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6177	0.3429776478740605	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	5840	0.3242657379932837	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5816	0.32293313907002363	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5610	0.31149499831204136	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	5459	0.3031107300865301	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	5438	0.3019447060286775	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4628	0.2569694923686502	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4603	0.25558136849025426	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	4566	0.25352694515022833	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4092	0.22720811641584196	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	4069	0.2259310424477177	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	4059	0.22537579289635934	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	3946	0.21910147296600987	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	3899	0.21649180007462554	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3461	0.19217186972512929	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	2991	0.16607514081128624	RNA PCR Primer, Index 3 (95% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT	2817	0.15641379861765073	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	2799	0.15541434942520566	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2574	0.1429212345196425	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT	2571	0.142754659654235	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	2523	0.14008946180771487	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	2447	0.1358695652173913	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2369	0.1315386187167961	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	2259	0.12543087365185407	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	2177	0.1208778273307155	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	2144	0.11904550381123293	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	2049	0.11377063307332848	No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC	1886	0.10472006538618717	No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA	1823	0.10122199321262948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	5.552495513583625E-5	0.0
7	0.0	0.0	0.0	1.110499102716725E-4	0.0
8	0.0	0.0	0.0	1.6657486540750874E-4	0.0
9	0.0	0.0	0.0	3.331497308150175E-4	0.0
10-11	0.0	0.0	0.0	4.719621186546081E-4	0.0
12-13	0.0	0.0	0.0	7.218244167658713E-4	0.0
14-15	0.0	0.0	0.0	8.328743270375438E-4	0.0
16-17	0.0	0.0	5.552495513583625E-5	9.439242373092162E-4	0.0
18-19	0.0	0.0	5.552495513583625E-5	0.00133259892326007	0.0
20-21	0.0	0.0	5.552495513583625E-5	0.00533039569304028	0.0
22-23	0.0	0.0	5.552495513583625E-5	0.01851757253780139	0.0
24-25	0.0	0.0	5.552495513583625E-5	0.038922993550221205	0.0
26-27	0.0	0.0	5.552495513583625E-5	0.07304307848119258	0.0
28-29	0.0	0.0	5.552495513583625E-5	0.20488708445123577	0.0
30-31	0.0	0.0	5.552495513583625E-5	0.47618201524493164	0.0
32-33	0.0	0.0	5.552495513583625E-5	0.9471724471846628	0.0
34-35	0.0	0.0	5.552495513583625E-5	1.6938442813738206	0.0
36-37	0.0	0.0	5.552495513583625E-5	2.5795506032231126	0.0
38-39	0.0	0.0	5.552495513583625E-5	3.7069570547787	0.0
40-41	0.0	0.0	5.552495513583625E-5	5.2751761251576905	0.0
42-43	0.0	0.0	5.552495513583625E-5	6.440228496295376	0.0
44-45	0.0	0.0	5.552495513583625E-5	7.444647172225085	0.0
46-47	0.0	0.0	5.552495513583625E-5	8.630660213926546	0.0
48-49	0.0	0.0	5.552495513583625E-5	9.718866047156233	0.0
50-51	0.0	0.0	5.552495513583625E-5	10.829948161901886	0.0
52-53	0.0	0.0	5.552495513583625E-5	11.968626179350046	0.0
54-55	0.0	0.0	5.552495513583625E-5	13.044116797853627	0.0
56-57	0.0	0.0	5.552495513583625E-5	14.202950374016098	0.0
58-59	0.0	0.0	5.552495513583625E-5	15.23374340363533	0.0
60-61	0.0	0.0	5.552495513583625E-5	16.183025799115153	0.0
62-63	0.0	0.0	5.552495513583625E-5	17.088637817380643	0.0
64-65	0.0	0.0	5.552495513583625E-5	18.07401143369876	0.0
66-67	0.0	0.0	5.552495513583625E-5	19.089424050745365	0.0
68-69	0.0	0.0	5.552495513583625E-5	20.318829844885485	0.0
70-71	0.0	0.0	5.552495513583625E-5	21.93277371581884	0.0
72-73	0.0	0.0	5.552495513583625E-5	23.156460439579966	0.0
74-75	0.0	0.0	5.552495513583625E-5	24.25146807981379	0.0
76-77	0.0	0.0	5.552495513583625E-5	25.33659227803344	0.0
78-79	0.0	0.0	5.552495513583625E-5	26.57007915637604	0.0
80-81	0.0	0.0	5.552495513583625E-5	27.801955811019702	0.0
82-83	0.0	0.0	5.552495513583625E-5	29.208958174161793	0.0
84-85	0.0	0.0	5.552495513583625E-5	30.542639834047016	0.0
86-87	0.0	0.0	5.552495513583625E-5	31.661828592242497	0.0
88	0.0	0.0	5.552495513583625E-5	32.64939544428848	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	75	4.1836756E-11	56.391804	7
GTACCCA	900	0.0	47.540398	1
TGTACCG	85	7.943527E-9	44.228867	5
TACCCAT	980	0.0	44.120937	2
GACGTTA	130	0.0	43.37831	7
ACCGTAT	90	1.31240085E-8	41.78563	8
GTTAATG	45	0.00101428	41.776344	2
ACCCATG	1040	0.0	41.119022	3
CGTTAAC	140	0.0	40.293285	9
GTACCGT	105	1.1423253E-9	40.279858	6
ATTCAAG	160	0.0	38.202103	1
GCGTATC	50	0.0016990901	37.614376	1
AGTCCCG	50	3.328023E-8	37.602886	46-47
ACTGTAC	140	1.2732926E-11	36.923206	3
ATACGGC	65	1.4150164E-4	36.148594	3
TACGGCT	80	1.1601682E-5	35.244877	4
AGACGTT	180	0.0	33.939514	6
GGTACTA	35	2.1844034E-4	33.575405	20-21
ACGTTAA	170	3.6379788E-12	33.182705	8
CAAGACG	170	3.6379788E-12	33.17165	4
>>END_MODULE