FastQCFastQC Report
Mon 6 Jun 2016
ERR522929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309222
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437330.3285917088166386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359830.2703614080522513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217240.16322516823297412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG179600.047.5217482
GTACCTG207750.041.953311
ACCTGGG212500.039.390343
CCTGGGG196050.028.2033274
GTATCAA606450.025.6257671
GGTATCA438650.025.3382971
CATGGGG349850.025.2794024
GTACATG603050.024.3116991
ATGGGGG225100.024.3112815
TACATGG619950.023.2257982
TGGGGGG228050.022.7910296
ACATGGG622300.022.753053
TCAACGC691100.022.1583144
CAACGCA697450.021.9483415
ATCAACG699650.021.8909993
TATAACG10850.021.6701742
TATCAAC707600.021.664662
AACGCAG717350.021.4872886
TATCACG11050.020.8523942
GTACACG26850.020.4962021
Produced by FastQC (version 0.11.5)