FastQCFastQC Report
Mon 6 Jun 2016
ERR522929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309222
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397530.29868763177892743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351990.2644707556910539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210660.15828122785839774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG182400.047.7715262
GTACCTG216450.040.6676941
ACCTGGG213450.039.959253
CCTGGGG191200.029.9361744
GGTATCA467750.024.6193331
GTATCAA624000.023.9254671
CATGGGG337750.023.7784544
GTACATG607950.022.7240241
ATGGGGG218850.022.7181385
TGGGGGG216300.022.3556656
TACATGG622100.021.8485342
CTGGGGG131100.021.5071185
ACATGGG624350.021.1878643
TCAACGC713050.020.667744
CAACGCA722250.020.3913085
TATAACG11100.020.3236852
ATCAACG725050.020.3062323
TATCAAC738150.020.0690042
AACGCAG739650.020.057516
GAGTACT397650.019.91277312-13
Produced by FastQC (version 0.11.5)